[1]安梦宇,帕提麦·马秉成,艾则孜·艾海提,等.甘草酸对骨关节炎大鼠炎症抑制与软骨保护作用[J].中国中医骨伤科杂志,2026,34(04):7-13.[doi:10.20085/j.cnki.issn1005-0205.260402]
　AN Mengyu,PATIMER Ma Bingcheng,AIZEZI Aihaiti,et al.Glycyrrhizic Acid Inhibits Inflammation and Protects Cartilage in Osteoarthritis Rats by the Regulating NF-κB/NLRP3 Pathway[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(04):7-13.[doi:10.20085/j.cnki.issn1005-0205.260402]
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甘草酸对骨关节炎大鼠炎症抑制与软骨保护作用()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年04期
页码:
7-13
栏目:
基础研究
出版日期:
2026-04-10

文章信息/Info

Title:
Glycyrrhizic Acid Inhibits Inflammation and Protects Cartilage in Osteoarthritis Rats by the Regulating NF-κB/NLRP3 Pathway
文章编号:
1005-0205(2026)04-0007-07
作者:
安梦宇1; 帕提麦·马秉成1; 艾则孜·艾海提1; 孟晓源1; 张浩沙强1; 宁凯1△
1新疆维吾尔自治区人民医院(乌鲁木齐,830000)
Author(s):
AN Mengyu1; PATIMER Ma Bingcheng1; AIZEZI Aihaiti1; MENG Xiaoyuan1; ZHANG Haosha Qiang1; NING Kai1△
1Xinjiang Uygur Autonomous Region People's Hospital,Urumqi 830000,China.
关键词:
甘草酸;  核因子κB/核苷酸结合寡聚结构域样受体蛋白3;  骨关节炎;  炎症;  细胞凋亡
Keywords:
glycyrrhizic acid;  NF-κB/NLRP3;  osteoarthritis;  inflammation;  apoptosis
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260402
文献标志码:
A
摘要:
目的:探究甘草酸(GA)调节核因子κB(NF-κB)/核苷酸结合寡聚结构域样受体蛋白3(NLRP3)信号通路对骨关节炎(OA)大鼠炎症反应和软骨细胞凋亡的影响。方法:首先建立骨关节炎大鼠模型,将建模成功的大鼠随机分为5组,即骨关节炎组、甘草酸低/中/高剂量组(GA-L组、GA-M组、GA-H组)以及高剂量甘草酸+NF-κB/NLRP3信号通路激活剂PMA组(GA-H+PMA组),另外随机选取等量只进行假手术的SD大鼠为阴性对照组。检测各组大鼠膝关节肿胀度和被动活动度; 检测各组大鼠压痛阈值和热痛阈值; 检测各组大鼠血清炎性相关因子及软骨寡聚基质蛋白(COMP)水平变化; 检测各组大鼠软骨组织病理及细胞凋亡变化,并进行Mankin评分; 检测各组大鼠软骨组织凋亡、基质金属蛋白酶13(MMP-13)及NF-κB/NLRP3信号通路相关蛋白表达。结果:骨关节炎组大鼠软骨组织结构破坏严重,软骨细胞减少且排列紊乱,被动活动度、压痛阈值、热痛阈值、Bcl2低于阴性对照组,膝关节肿胀度、细胞凋亡率、Mankin评分、IL-18、IL-6、IL-1β、COMP、Bax、MMP-13、p-NF-κB p65/NF-κB p65、NLRP3、Caspase-1高于阴性对照组,差异有统计学意义(P<0.05); GA-L组、GA-M组、GA-H组大鼠软骨组织破坏及软骨细胞损伤减轻,被动活动度、压痛阈值、热痛阈值、Bcl2高于骨关节炎组,膝关节肿胀度、细胞凋亡率、Mankin评分、IL-18、IL-6、IL-1β、COMP、Bax、MMP-13、p-NF-κB p65/NF-κB p65、NLRP3、Caspase-1低于骨关节炎组,差异有统计学意义(P<0.05); GA-H+PMA组大鼠软骨组织破坏及软骨细胞损伤加重,被动活动度、压痛阈值、热痛阈值、Bcl2低于GA-H组,膝关节肿胀度、细胞凋亡率、Mankin评分、IL-18、IL-6、IL-1β、COMP、Bax、MMP-13、p-NF-κB p65/NF-κB p65、NLRP3、Caspase-1高于GA-H组,差异有统计学意义(P<0.05)。结论:甘草酸可能通过抑制NF-κB/NLRP3信号通路,减少骨关节炎大鼠炎症反应和软骨细胞凋亡,延缓骨关节退化。
Abstract:
Objective:To investigate the effects of glycyrrhizic acid(GA)on inflammatory response and chondrocyte apoptosis in osteoarthritis(OA)rats via regulation of the nuclear factor-κB(NF-κB)/nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)signaling pathway.Methods:Firstly,osteoarthritis rats were established,Then the successfully modeled rats were randomly separated into five groups:OA group,GA low,medium,and high dose groups(GA-L group,GA-M group,GA-H group),and GA high-dose+NF-κB/NLRP3 signaling pathway activator PMA group(GA-H+PMA group).In addition,an equal number of SD rats undergoing sham surgery were randomly considered as the negative control(NC)group.The knee joint swelling and passive range of motion in rats from each group were measured.The tenderness threshold and thermal pain threshold of rats were determined.The changes in serum levels of inflammatory-related factors and cartilage oligomeric matrix protein(COMP)were detected in rats.The pathological and apoptotic changes of cartilage tissue in rats were detected by Mankin score.The apoptosis of cartilage tissue,the expression of matrix metalloproteinase-13(MMP-13)and NF-κB/NLRP3 signaling pathway related proteins were detected in each group.Results:The cartilage tissue structure of rats in the OA group was severely damaged,with reduced and disordered arrangement of chondrocytes.The passive range of motion,tenderness threshold,thermal pain threshold,and Bcl2 were lower than those in the NC group,and the knee joint swelling,cell apoptosis rate,Mankin score,IL-18,IL-6,IL-1β,COMP,Bax,MMP-13,p-NF-κB p65/NF-κB p65,NLRP3,and Caspase-1 were higher than those in the NC group(P<0.05).The destruction of cartilage tissue and damage to chondrocytes in rats in the GA-L group,GA-M group,and GA-H group were reduced,the passive range of motion,tenderness threshold,thermal pain threshold,and Bcl2 were higher than those in the OA group,and the knee joint swelling,cell apoptosis rate,Mankin score,IL-18,IL-6,IL-1β,COMP,Bax,MMP-13,p-NF-κB p65/NF-κB p65,NLRP3,and Caspase-1 were lower than those in the OA group(P<0.05).The destruction of cartilage tissue and damage to chondrocytes in rats in the GA-H+PMA group were aggravated,the passive range of motion,tenderness threshold,thermal pain threshold,and Bcl2 were lower than those in the GA-H group,and the knee joint swelling,cell apoptosis rate,Mankin score,IL-18,IL-6,IL-1β,COMP,Bax,MMP-13,p-NF-κB p65/NF-κB p65,NLRP3,and Caspase-1 were higher than those in the GA-H group(P<0.05).Conclusion:Glycyrrhizic acid may inhibit the NF-κB/NLRP3 signaling pathway to reduce inflammation and chondrocyte apoptosis in osteoarthritis rats,and delay bone and joint degeneration.

参考文献/References:

[1] JIANG Y.Osteoarthritis year in review 2021:biology[J].Osteoarthritis and Cartilage,2022,30(2):207-215.
[2] MOTTA F,BARONE E,SICA A,et al.Inflammaging and osteoarthritis[J].Clinical Reviews in Allergy & Immunology,2023,64(2):222-238.
[3] LU J,GU X Y,XUE C,et al.Glycyrrhizic acid alleviates concanavalin A-induced acute liver injury by regulating monocyte-derived macrophages[J].Phytomedicine,2024,133:155586.
[4] LI C,LI L,LAN T.Co-treatment with disulfiram and glycyrrhizic acid suppresses the inflammatory response of chondrocytes[J].Journal of Orthopaedic Surgery and Research,2021,16(1):132.
[5] CUI L K,SHEN G B,YU Y,et al.Gubi decoction mitigates knee osteoarthritis via promoting chondrocyte autophagy through METTL3-mediated ATG7 m6A methylation[J].Journal of Cellular and Molecular Medicine,2024,28(16):e70019.
[6] LOU C,FANG Y Q,MEI Y F,et al.Cucurbitacin B attenuates osteoarthritis development by inhibiting NLRP3 inflammasome activation and pyroptosis through activating Nrf2/HO-1 pathway[J].Phytotherapy Research,2024,38(7):3352-3369.
[7] 金剑飞,何维英,周钰龙.淫羊藿总黄酮对膝关节骨性关节炎大鼠核因子-κB通路的影响[J].中国中医骨伤科杂志,2020,28(3):5-9.
[8] 郭跃生,穆岭,张锟,等.苍术内酯对骨关节炎大鼠软骨损伤修复和软骨细胞凋亡的影响[J].安徽医药,2023,27(8):1497-1502.
[9] 胡志平,何绍前,王传明,等.甘草酸抑制PI3K/AKT/NF-κB减轻胶原诱导型类风湿关节炎大鼠炎症反应[J].中国老年学杂志,2020,40(13):2852-2856.
[10] 张昕悦,尹帅,张海峰,等.根皮苷对前交叉韧带横断诱导的大鼠骨关节炎的影响[J].南开大学学报(自然科学版),2024,57(6):46-51.
[11] CHENG J H,CHOU W Y,WANG C J,et al.Pathological,morphometric and correlation analysis of the modified mankin score,tidemark roughness and calcified cartilage thickness in rat knee osteoarthritis after extracorporeal shockwave therapy[J].International Journal of Medical Sciences,2022,19(2):242-256.
[12] YUNUS M H M,NORDIN A,KAMAL H.Pathophysiological perspective of osteoarthritis[J].Medicina,2020,56(11):614.
[13] FUJII Y,LIU L,YAGASAKI L,et al.Cartilage homeostasis and osteoarthritis[J].International Journal of Molecular Sciences,2022,23(11):6316.
[14] SHAWL M,GEETHA T,BURNETT D,et al.Omega-3 supplementation and its effects on osteoarthritis[J].Nutrients,2024,16(11):1650.
[15] 彭博文,冯立,覃剑.贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响[J].中国骨质疏松杂志,2024,30(7):980-984.
[16] CHEN Q,WU C Y,WANG S W,et al.Glycyrrhizic acid modified Poria Cocos polyscaccharide carbon dots dissolving microneedles for methotrexate delivery to treat rheumatoid arthritis[J].Frontiers in Chemistry,2023,11:1181159.
[17] JIA Z Y,ZHANG J L,YANG X T,et al.Bioactive components and potential mechanisms of Biqi capsule in the treatment of osteoarthritis:based on chondroprotective and anti-inflammatory activity[J].Frontiers in Pharmacology,2024,15:1347970.
[18] 叶海东,刘宇,甄朋超.苍术素调节PINK1/Parkin信号通路对膝骨关节炎大鼠线粒体自噬的影响[J].微循环学杂志,2023,33(4):13-20.
[19] 涂仕娟,杨红亚,李桃,等.吴茱萸碱调节Shh/Gli1信号通路对膝骨关节炎大鼠软骨损伤的影响[J].中国医科大学学报,2024,53(9):827-833.
[20] JIANG P,ZHOU X N,YANG Y,et al.Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway[J].International Immunopharmacology,2024,140:112741.
[21] LI Z H,HUANG Z Y,ZHANG H,et al.P2X7 receptor induces pyroptotic inflammation and cartilage degradation in osteoarthritis via NF-κB/NLRP3 crosstalk[J].Oxidative Medicine and Cellular Longevity,2021:8868361.
(收稿日期:2025-08-08)

备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区人民医院院内项目(20230209)
△通信作者 E-mail:13579233099@163.com
更新日期/Last Update: 2026-04-15