[1]王亮△,许治国,杨轶.橘红素对白细胞介素-1β诱导的软骨细胞损伤的影响[J].中国中医骨伤科杂志,2026,34(03):51-56.[doi:10.20085/j.cnki.issn1005-0205.260308]
　WANG Liang,XU Zhiguo,YANG Yi.The Effect of Tangeretin on Interleukin-1β-Induced Chondrocyte Damage by Regulating the AMPK/SIRT1/NF-κB Signaling Pathway[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(03):51-56.[doi:10.20085/j.cnki.issn1005-0205.260308]
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橘红素对白细胞介素-1β诱导的软骨细胞损伤的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年03期
页码:
51-56
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
The Effect of Tangeretin on Interleukin-1β-Induced Chondrocyte Damage by Regulating the AMPK/SIRT1/NF-κB Signaling Pathway
文章编号:
1005-0205(2026)03-0051-06
作者:
王亮1△; 许治国1; 杨轶1
1唐山市中医医院(河北 唐山,063000)
Author(s):
WANG Liang1△; XU Zhiguo1; YANG Yi1
1Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,Hebei China.
关键词:
橘红素;  AMPK/SIRT1/NF-κB通路;  白细胞介素-1β;  软骨细胞;  损伤
Keywords:
tangeretin;  AMPK/SIRT1/NF-κB pathway;  interleukin-1β;  chondrocytes;  damage
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260308
文献标志码:
A
摘要:
目的:探究橘红素对白细胞介素-1β(IL-1β)诱导的软骨细胞损伤的影响,分析其机制与AMP激活蛋白激酶(AMPK)/沉默信息调节因子2相关酶1(SIRT1)/核因子κB(NF-κB)通路的关系。方法:培养软骨细胞,并分为对照组、白细胞介素-1β组、低/中/高剂量橘红素组、高剂量橘红素+AMPK抑制剂Compound C组(高剂量橘红素+Compound C组)。用克隆形成实验、流式细胞术、DCFH-DA荧光探针、Western Blot法分别检测细胞增殖、凋亡、活性氧(ROS)水平、Collagen Ⅱ、MMP-3、MMP-13及AMPK/SIRT1/NF-κB通路相关蛋白表达。结果:白细胞介素-1β组较对照组克隆形成数量、SOD水平、Collagen Ⅱ、SIRT1表达、p-AMPK/AMPK比值降低,细胞凋亡率、IFN-γ、IL-6、TNF-α、MDA水平、荧光强度、MMP-3、MMP-13表达、Ac-NF-κB p65/NF-κB p65比值升高,差异有统计学意义(P<0.05); 低/中/高剂量橘红素组较白细胞介素-1β组克隆形成数量、SOD水平、Collagen Ⅱ、SIRT1表达、p-AMPK/AMPK比值升高,细胞凋亡率、IFN-γ、IL-6、TNF-α、MDA水平、荧光强度、MMP-3、MMP-13表达、Ac-NF-κB p65/NF-κB p65比值降低,差异有统计学意义(P<0.05); 高剂量橘红素+Compound C组较高剂量橘红素组克隆形成数量、SOD水平、Collagen Ⅱ、SIRT1表达、p-AMPK/AMPK比值降低,细胞凋亡率、IFN-γ、IL-6、TNF-α、MDA水平、荧光强度、MMP-3、MMP-13表达、Ac-NF-κB p65/NF-κB p65比值升高,差异有统计学意义(P<0.05)。结论:橘红素对IL-1β诱导的软骨细胞损伤的改善作用可能通过激活AMPK/SIRT1/NF-κB通路实现。
Abstract:
Objective:To investigate the effect of tangeretin on interleukin-1β(IL-1β)-induced chondrocyte injury and analyze its mechanistic association with the AMP-activated protein kinase(AMPK)/silent information regulator 2 homolog 1(SIRT1)/nuclear factor κB(NF-κB)signaling pathway.Methods:Chondrocytes were cultured and divided into control group,interleukin-1β group,low-dose,medium-dose,and high-dose tangeretin groups,and high-dose tangeretin plus AMPK inhibitor Compound C group.Cloning formation assays,flow cytometry,DCFH-DA fluorescent probes,and Western Blot were used to detect cell proliferation,apoptosis,ROS levels,Collagen Ⅱ,MMP-3,MMP-13,and the expression of proteins related to the AMPK/SIRT1/NF-κB pathway.Results:The interleukin-1β group showed reduced clone formation number,SOD levels,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,as well as increased apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared to the control group(P<0.05).The low-dose,medium-dose,and high-dose tangeretin groups showed increased clone formation number,SOD levels,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,as well as reduced apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared with the interleukin-1β group(P<0.05).The high-dose tangeretin+Compound C group showed reduced clone formation number,SOD level,Collagen Ⅱ,SIRT1 expression,p-AMPK/AMPK ratio,increased apoptosis rate,IFN-γ,IL-6,TNF-α,MDA levels,fluorescence intensity,MMP-3,MMP-13 expression,and Ac-NF-κB p65/NF-κB p65 ratio compared to the high dose tangeretin group(P<0.05).Conclusion:The improvement effect of tangeretin on IL-1β-induced chondrocyte damage may be achieved by activating the AMPK/SIRT1/NF-κB signaling pathway.

参考文献/References:

[1] LI Z,CHENG J Q,LIU J X.Baicalin protects human OA chondrocytes against IL-1β-induced apoptosis and ECM degradation by activating autophagy via miR-766-3p/AIFM1 axis[J].Drug Design,Development and Therapy,2020,14:2645-2655.
[2] 程晏,石文俊,张磊,等.瘀血痹片联合抗骨质疏松症药物对膝骨关节炎的临床疗效及膝关节功能评分的影响研究[J].中国骨质疏松杂志,2021,27(6):882-885.
[3] ZHUANG H M,REN X S,JIANG F Z,et al.Indole-3-propionic acid alleviates chondrocytes inflammation and osteoarthritis via the AhR/NF-κB axis[J].Molecular Medicine,2023,29(1):17.
[4] 左淑飞,梁舒,秦艺璐,等.微小RNA-6779-5p对白细胞介素-1β诱导软骨细胞损伤的影响及机制研究[J].实用临床医药杂志,2023,27(12):69-75.
[5] CHEN B,LUO J K,HAN Y H,et al.Dietary tangeretin alleviated dextran sulfate sodium-induced colitis in mice via inhibiting inflammatory response,restoring intestinal barrier function,and modulating gut microbiota[J].Journal of Agricultural and Food Chemistry,2021,69(27):7663-7674.
[6] 王西彬,左瑞庭,王新立,等.钩藤碱通过Nrf2/HO-1信号通路抑制IL-1β诱导的软骨细胞损伤[J].中国骨质疏松杂志,2023,29(7):966-970.
[7] 黄慧灵,陈珺,田欣,等.橘红素对大鼠成骨细胞增殖、分化和成骨功能的影响[J].中国老年学杂志,2023,43(11):2715-2720.
[8] 胡宏志,汪能,李娟,等.青藤碱调节AMPK/mTOR/ULK1信号通路对IL-1β诱导的关节软骨细胞自噬和凋亡的影响[J].疑难病杂志,2024,23(11):1379-1384.
[9] LIN Z M,MIAO J N,ZHANG T,et al.JUNB-FBXO21-ERK axis promotes cartilage degeneration in osteoarthritis by inhibiting autophagy[J].Aging Cell,2021,20(2):e13306.
[10] CHEN W,ZHENG H T,ZHANG X,et al.Columbianetin alleviates lipopolysaccharides(LPS)-induced inflammation and apoptosis in chondrocyte through activation of autophagy by inhibiting serum and glucocorticoid-induced protein kinase 1(SGK1)expression[J].Bioengineered,2022,13(2):4051-4062.
[11] PENG B R,HU J W,SUN Y F,et al.Tangeretin alleviates inflammation and oxidative response induced by spinal cord injury by activating the Sesn2/Keap1/Nrf2 pathway[J].Phytotherapy Research,2024,38(9):4555-4569.
[12] SHI Y F,CHEN J X,LI S L,et al.Tangeretin suppresses osteoarthritis progression via the Nrf2/NF-κB and MAPK/NF-κB signaling pathways[J].Phytomedicine,2022,98:153928.
[13] SONG B,SONG H,WANG W G,et al.Beclin 1 overexpression inhibits chondrocyte apoptosis and downregulates extracellular matrix metabolism in osteoarthritis[J].Molecular Medicine Reports,2017,16(4):3958-3964.
[14] 包喜文,李振英,吴霞,等.黑骨藤追风活络胶囊对胶原诱导性关节炎大鼠IL-1β、IFN-γ、IL-15的影响[J].中国中医急症,2018,27(2):293-295.
[15] 李广贤,乔卫平.金骨莲胶囊治疗骨性关节炎疗效及对患者IL-1、IL-6、TNF-α影响的研究[J].陕西中医,2018,39(12):1792-1794.
[16] ANSARI M Y,AHMAD N,HAQQI T M.Oxidative stress and inflammation in osteoarthritis pathogenesis:role of polyphenols[J].Biomedicine & Pharmacotherapy,2020,129:110452.
[17] NI S,LI D,WEI H,et al.PPARγ attenuates interleukin-1β-induced cell apoptosis by inhibiting NOX2/ROS/p38MAPK activation in osteoarthritis chondrocytes[J].Oxidative Medicine and Cellular Longevity,2021(1):5551338.
[18] YANG Y T,SHEN P Y,YAO T,et al.Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress[J].Theranostics,2021,11(4):1877-1900.
[19] PENG Z,SUN H,BUNPETCH V,et al.The regulation of cartilage extracellular matrix homeostasis in joint cartilage degeneration and regeneration[J].Biomaterials,2021,268:120555.
[20] ZHU X B,CHEN F,LU K,et al.PPARγ preservation via promoter demethylation alleviates osteoarthritis in mice[J].Annals of the Rheumatic Diseases,2019,78(10):1420-1429.
[21] ZHOU Y L,LI J,WANG C L,et al.Fumitremorgin C alleviates advanced glycation end products(AGE)-induced chondrocyte inflammation and collagen Ⅱ and aggrecan degradation through sirtuin-1(SIRT1)/nuclear factor(NF)-κB/mitogen-activated protein kinase(MAPK)[J].Bioengineered,2022,13(2):3867-3876.
[22] BERKE I M,JAIN E,YAVUZ B,et al.NF-κB-mediated effects on behavior and cartilage pathology in a non-invasive loading model of post-traumatic osteoarthritis[J].Osteoarthritis and Cartilage,2021,29(2):248-256.
[23] YE Q Y,XU H K,LIU S Y,et al.Apoptotic extracellular vesicles alleviate Pg-LPS induced inflammatory responses of macrophages via AMPK/SIRT1/NF-κB pathway and inhibit osteoclast formation[J].Journal of Periodontology,2022,93(11):1738-1751.
(收稿日期:2025-08-07)

备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2024425)
△通信作者 E-mail:ej74898@163.com
更新日期/Last Update: 2026-03-15