[1]何德利△,陈宏慈,陈凯奇.复方生脉成骨胶囊调控miR-135b-5p对小鼠激素性股骨头坏死及骨代谢的影响[J].中国中医骨伤科杂志,2026,34(03):43-50.[doi:10.20085/j.cnki.issn1005-0205.260307]
　HE Deli,CHEN Hongci,CHEN Kaiqi.The Effect of Compound Shengmai Chenggu Capsules on miR-135b-5p in the Regulating of Steroid-Induced Femoral Head Necrosis and Bone Metabolism in Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(03):43-50.[doi:10.20085/j.cnki.issn1005-0205.260307]
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复方生脉成骨胶囊调控miR-135b-5p对小鼠激素性股骨头坏死及骨代谢的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年03期
页码:
43-50
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
The Effect of Compound Shengmai Chenggu Capsules on miR-135b-5p in the Regulating of Steroid-Induced Femoral Head Necrosis and Bone Metabolism in Mice
文章编号:
1005-0205(2026)03-0043-08
作者:
何德利1△; 陈宏慈1; 陈凯奇1
1广州中医药大学番禺医院(广州,511400)
Author(s):
HE Deli1△; CHEN Hongci1; CHEN Kaiqi1
1Panyu Hospital,Guangzhou University of Chinese Medicine,Guangzhou 511400,China.
关键词:
补肾活血;  激素性股骨头坏死;  骨代谢;  阿仑膦酸钠;  复方生脉成骨胶囊
Keywords:
Bushen Huoxue;  steroid-induced femoral head necrosis;  bone metabolism;  alendronate sodium;  compound Shengmai Chenggu capsules
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260307
文献标志码:
A
摘要:
目的:基于补肾活血理论研究复方生脉成骨胶囊对小鼠激素性股骨头坏死的改善作用及机制。方法:小鼠随机分为正常组和造模组,采用脂多糖联合甲强龙建立激素性股骨头坏死模型。造模完成后随机分为模型组、复方生脉成骨胶囊组、Agomir组、复方生脉成骨胶囊+Agomir组和阿仑膦酸钠组,12只/组。复方生脉成骨胶囊组灌胃给予小鼠复方生脉成骨胶囊(5.46 g/kg),1次/d; Agomir组按照200 μL/只,尾静脉注射miR-135b-5p Agomir(14 nmol/L),每2周1次; 复方生脉成骨胶囊+Agomir组灌胃给予复方生脉成骨胶囊(5.46 g/kg),1次/d,同时尾静脉注射miR-135b-5p Agomir(14 nmol/L),每2周1次; 阿仑膦酸钠组灌胃给予阿仑膦酸钠(1.3 mg/kg),1次/d; 正常组和模型组灌胃给予等量蒸馏水,所有组别连续干预8周。实时定量聚合酶链式反应(PCR)测定miR-135b-5p水平,微型计算机断层扫描仪(Micro-CT)检查小鼠股骨头形态并计算骨小梁数量、骨小梁厚度和骨小梁分离度,酶联免疫吸附法(ELISA)试剂盒测定小鼠血清骨钙素、碱性磷酸酶(ALP)、Ⅰ型胶原氨基端前肽(PINP)、抗酒石酸酸性磷酸酶(TRAP)、甲状旁腺素(PTH)、骨碱性磷酸酶(BALP)水平,全自动生化分析仪测定小鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、超氧化物歧化酶(SOD)、丙二醛(MDA)和脂质过氧化物(LPO)水平,苏木精-伊红(HE)染色法检查小鼠股骨头组织病理改变,实时定量聚合酶链式反应和免疫印迹法分别测定小鼠股骨头Runt相关转录因子2(Runx2)、骨形态发生蛋白2(BMP-2)、成骨相关转录因子抗体(Osterix)mRNA和蛋白水平。结果:与正常组比较,模型组miR-135b-5p水平、骨小梁分离度、血清ALP、TRAP、PTH、TNF-α、IL-6、MDA和LPO水平升高,差异有统计学意义(P<0.05); 股骨头骨小梁数量、骨小梁厚度、血清骨钙素、PINP、BALP和SOD水平,Runx2、BMP-2、Osterix mRNA和蛋白水平降低,差异有统计学意义(P<0.05)。与模型组比较,复方生脉成骨胶囊组和阿仑膦酸钠组miR-135b-5p水平、骨小梁分离度、血清ALP、TRAP、PTH、TNF-α、IL-6、MDA和LPO水平降低,差异有统计学意义(P<0.05); 股骨头骨小梁数量、骨小梁厚度、血清骨钙素、PINP、BALP和SOD水平,Runx2、BMP-2、Osterix mRNA和蛋白水平升高,差异有统计学意义(P<0.05),股骨头组织病理结构显著改善。与正常组、模型组和复方生脉成骨胶囊组比较,Agomir组miR-135b-5p水平、骨小梁分离度、血清ALP、TRAP、PTH、TNF-α、IL-6、MDA和LPO水平升高,差异有统计学意义(P<0.05); 股骨头骨小梁数量、骨小梁厚度、血清骨钙素、PINP、BALP和SOD水平,Runx2、BMP-2、Osterix mRNA和蛋白水平降低,差异有统计学意义(P<0.05)。与Agomir组比较,复方生脉成骨胶囊+Agomir组miR-135b-5p水平、骨小梁分离度、血清ALP、TRAP、PTH、TNF-α、IL-6、MDA和LPO水平降低,差异有统计学意义(P<0.05); 股骨头骨小梁数量、骨小梁厚度、血清骨钙素、PINP、BALP和SOD水平,Runx2、BMP-2、Osterix mRNA和蛋白水平升高,差异有统计学意义(P<0.05)。结论:复方生脉成骨胶囊能够显著抑制激素性股骨头坏死小鼠股骨头组织结构破坏,促进股骨头病理恢复,调节骨代谢,抑制小鼠全身炎症反应及氧化应激损伤,促进股骨头成骨细胞分化,其机制可能与调节miR-135b-5p有关。
Abstract:
Objective:To study the improvement effect and mechanism of compound Shengmai Chenggu capsules on steroid-induced femoral head necrosis in mice based on the Bushen Huoxue theory.Methods:Mice were randomly divided into the normal group and the model group.The steroid-induced femoral head necrosis model was established using lipopolysaccharide combined with methylprednisolone.After the modeling was completed,they were randomly divided into the model group,the compound Shengmai Chenggu capsules group,the Agomir group,the compound Shengmai Chenggu capsules+Agomir group,and the alendronate sodium group,with 12 rats in each group.In the compound Shengmai Chenggu capsules group,mice received compound Shengmai Chenggu capsules(5.46 g/kg)by gavage once a day.In the Agomir group,miR-135b-5p Agomir(14 nmol/L)was injected into the tail vein at a volume of 200 μL per mouse once for 2 weeks.In the compound Shengmai Chenggu capsules+Agomir group,compound Shengmai Chenggu capsules(5.46 g/kg)were administered intragastrically once a day,and miR-135b-5p Agomir(14 nmol/L)was injected into the tail vein once for 2 weeks.In the alendronate sodium group,alendronate sodium(1.3 mg/kg)was administered intragastrically once a day.The normal group and the model group were administered an equivalent volume of distilled water by gavage,all groups were continuously treated for 8 weeks.The level of miR-135b-5p was determined by real-time quantitative polymerase chain reaction(qPCR),the morphology of the femoral head in mice was examined using a micro-computed tomography scanner(Micro-CT),and the number of trabeculae,the thickness of trabeculae and the separation of trabeculae were calculated.The levels of osteocalcin,alkaline phosphatase(ALP),type Ⅰ collagen amino-terminal propeptide(PINP),tartrate-resistant acid phosphatase(TRAP),parathyroid hormone(PTH),and bone alkaline phosphatase(BALP)in mouse serum were determined using enzyme-linked immunosorbent assay(ELISA)kits.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),superoxide dismutase(SOD),malondialdehyde(MDA),and lipid peroxide(LPO)in mouse serum were determined using an automatic biochemical analyzer.The histopathological changes in the femoral head of mice were examined by hematoxylin-eosin(HE)staining.The mRNA and protein levels of Runt-related transcription factor 2(Runx2),bone morphogenetic protein 2(BMP-2),and osteogenic transcription factor antibody(Osterix)in the femoral head of mice were determined by real-time quantitative PCR and Western Blot,respectively.Results:Compared with the normal group,the levels of miR-135b-5p,trabecular bone separation,serum ALP,TRAP,PTH,TNF-α,IL-6,MDA and LPO in the model group were increased(P<0.05); the number of trabeculae in the femoral head,trabecular thickness,the levels of serum osteocalcin,PINP,BALP and SOD,as well as the mRNA and protein levels of Runx2,BMP-2 and Osterix were decreased(P<0.05).Compared with the model group,the levels of miR-135b-5p,trabecular bone separation,serum ALP,TRAP,PTH,TNF-α,IL-6,MDA,and LPO in both compound Shengmai Chenggu capsules group and the alendronate sodium group were decreased(P<0.05),the number of trabeculae of the femoral head,the thickness of trabeculae,the levels of serum osteocalcin,PINP,BALP and SOD,the mRNA and protein levels of Runx2,BMP-2 and Osterix increased(P<0.05),and the histopathological structure of the femoral head tissue was significantly improved.Compared with the normal group,the model group and compound Shengmai Chenggu capsules group,the levels of miR-135b-5p,trabecular bone separation,serum ALP,TRAP,PTH,TNF-α,IL-6,MDA and LPO in the Agomir group were increased(P<0.05).The number of trabeculae of the femoral head,the thickness of trabeculae,the levels of serum osteocalcin,PINP,BALP and SOD,as well as the mRNA and protein levels of Runx2,BMP-2 and Osterix decreased(P<0.05).Compared with the Agomir group,the levels of miR-135b-5p,trabecular bone separation,serum ALP,TRAP,PTH,TNF-α,IL-6,MDA and LPO in the compound Shengmai Chenggu capsules+Agomir group were decreased(P<0.05),the number of trabeculae of the femoral head,the thickness of trabeculae,the levels of serum osteocalcin,PINP,BALP and SOD,as well as the mRNA and protein levels of Runx2,BMP-2 and Osterix increased(P<0.05).Conclusion:The compound Shengmai Chenggu capsules can significantly inhibit the destruction of femoral head tissue structure in mice with steroid-induced femoral head necrosis,promote the pathological recovery of the femoral head,regulate bone metabolism,inhibit systemic inflammatory responses and oxidative stress injury in mice,and promote osteoblast differentiation in the femoral head.The mechanism may be related to the regulation of miR-135b-5p.

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(收稿日期:2025-06-25)

备注/Memo

备注/Memo:
基金项目:广东省中医药局科研项目(20232127)
△通信作者 E-mail:jiebi7917@sina.com
更新日期/Last Update: 2026-03-15