[1]许燕飞,王琪,李皎,等.牛膝多糖对激素性股骨头坏死大鼠骨代谢的影响[J].中国中医骨伤科杂志,2026,34(03):30-35.[doi:10.20085/j.cnki.issn1005-0205.260305]
　XU Yanfei,WANG Qi,LI Jiao,et al.Effect of Achyranthes Bidentata Polysaccharide on Bone Metabolism in Rats with Steroid-Induced Femoral Head Osteonecrosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(03):30-35.[doi:10.20085/j.cnki.issn1005-0205.260305]
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牛膝多糖对激素性股骨头坏死大鼠骨代谢的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年03期
页码:
30-35
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Effect of Achyranthes Bidentata Polysaccharide on Bone Metabolism in Rats with Steroid-Induced Femoral Head Osteonecrosis
文章编号:
1005-0205(2026)03-0030-06
作者:
许燕飞1; 王琪1; 李皎1; 郭英1; 郭磊1△
1昆明市中医医院(昆明,650500)
Author(s):
XU Yanfei1; WANG Qi1; LI Jiao1; GUO Ying1; GUO Lei1△
1Kunming Hospital of Traditional Chinese Medicine,Kunming 650500,China.
关键词:
牛膝多糖;  BMP-2/Smad5/Runx2信号通路;  激素性股骨头坏死;  骨代谢
Keywords:
Achyranthes bidentata polysaccharide;  BMP-2/Smad5/Runx2 signaling pathway;  steroid-induced osteonecrosis of the femoral head;  bone metabolism
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260305
文献标志码:
A
摘要:
目的:探究牛膝多糖对激素性股骨头坏死大鼠骨代谢的影响,分析其机制是否与骨形态发生蛋白2(BMP-2)/Smad5/Runt相关转录因子2(Runx2)信号通路有关。方法:将构建成功的激素性股骨头坏死大鼠随机分为模型组、低剂量牛膝多糖组(2.0 g/kg)、中剂量牛膝多糖组(4.0 g/kg)、高剂量牛膝多糖组(8.0 g/kg)、高剂量牛膝多糖+BMP-2抑制剂Noggin(高剂量牛膝多糖+Noggin)组(8.0 g/kg牛膝多糖+100 ng/kg Noggin),每组12只,另取12只大鼠作为对照组。用ELISA试剂盒检测大鼠骨代谢指标; 用双能X线吸收测量法测定大鼠骨密度(BMD)水平; HE染色检测大鼠股骨头组织病理形态; Western Blot法检测大鼠股骨头组织中BMP-2/Smad5/Runx2信号通路相关蛋白表达。结果:对照组大鼠股骨头形态结构正常; 模型组大鼠骨小梁空间结构紊乱,骨小梁纤细稀疏且空隙增大。模型组碱性磷酸酶(ALP)、Ⅰ型前胶原氨基端前肽(PINP)、骨钙素(BGP)、骨密度水平及BMP-2、p-Smad5/Smad5、Runx2表达低于对照组,抗酒石酸酸性磷酸酶(TRAP)、Ⅰ型胶原交联N-端肽(NTx)水平、空骨陷窝率高于对照组,差异有统计学意义(P<0.05); 低/中/高剂量牛膝多糖组ALP、PINP、BGP、骨密度水平及BMP-2、p-Smad5/Smad5、Runx2表达水平高于模型组,TRAP、NTx水平、空骨陷窝率低于模型组,且骨小梁形态有不同程度的改善,差异有统计学意义(P<0.05); 高剂量牛膝多糖+Noggin组ALP、PINP、BGP、骨密度水平、BMP-2、p-Smad5/Smad5、Runx2表达低于高剂量牛膝多糖组,TRAP、NTx水平、空骨陷窝率高于高剂量牛膝多糖组,且骨小梁形态损伤程度加重,差异有统计学意义(P<0.05)。结论:牛膝多糖可改善激素性股骨头坏死大鼠骨代谢,其机制可能与BMP-2/Smad5/Runx2通路激活有关。
Abstract:
Objective:To investigate the effects of Achyranthes bidentata polysaccharide on bone metabolism in steroid-induced osteonecrosis of the femoral head(SONFH)rats and to explore whether its mechanism is associated with the bone morphogenetic protein-2(BMP-2)/Smad5/Runt-related transcription factor 2(Runx2)signaling pathway.Methods:The successfully constructed SONFH rats were randomly divided into the following groups:model group,low-dose Achyranthes bidentata polysaccharide group(2.0 g/kg),medium-dose Achyranthes bidentata polysaccharide group(4.0 g/kg),high-dose Achyranthes bidentata polysaccharide group(8.0 g/kg),and high-dose Achyranthes bidentata polysaccharide+BMP-2 inhibitor Noggin group(8.0 g/kg Achyranthes bidentata polysaccharide+100 ng/kg Noggin),with 12 rats in each group.Another 12 rats were considered as the control group.An ELISA kit was used to detect bone metabolism indicators in rats.Dual-energy X-ray absorptiometry was performed to measure bone mineral density(BMD)in rats.HE staining was performed to examine the pathological morphology of rat femoral head tissue.Western Blot was performed to detect proteins related to the BMP-2/Smad5/Runx2 signaling pathway in rat femoral head tissue.Results:In the control group,the morphology and structure of the rat femoral head were normal.The spatial structure of the bone trabeculae in the model group was disrupted,with thin and sparse bone trabeculae and increased gaps.The model group had lower levels of ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2 than control group,and higher TRAP,NTx,and empty bone resorption rate than the control group(P<0.05).The low-dose,medium-dose,and high-dose Achyranthes bidentata polysaccharide groups exhibited higher levels of ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2,and lower levels of TRAP,NTx,and bone resorption pit rate than the model group,with the morphology of bone trabeculae showing varying degrees of improvement(P<0.05).The high-dose Achyranthes bidentata polysaccharide+Noggin group had lower ALP,PINP,BGP,BMD,BMP-2,p-Smad5/Smad5,and Runx2,and higher TRAP,NTx,and empty bone resorption rate than the high-dose Achyranthes bidentata polysaccharide group,and the degree of morphological damage to the bone trabeculae was more severe(P<0.05).Conclusion:Achyranthes bidentata polysaccharide can improve bone metabolism in SONFH rats,and the mechanism may be related to the activation of BMP-2/Smad5/Runx2 pathway.

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(收稿日期:2025-04-14)

备注/Memo

备注/Memo:
基金项目:云南省教育厅科学研究基金项目(2024Y417)
△通信作者 E-mail:guolei73@hotmail.com
更新日期/Last Update: 2026-03-15