[1]薛慧芝,曾家莹,陈晓聪 姚楠,等.参骨颗粒治疗绝经后骨质疏松的作用机制[J].中国中医骨伤科杂志,2026,34(03):16-22.[doi:10.20085/j.cnki.issn1005-0205.260303]
　XUE Huizhi,ZENG Jiaying,CHEN Xiaocong,et al.Exploring the Mechanism of Shengu Granules in Treating Postmenopausal Osteoporosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(03):16-22.[doi:10.20085/j.cnki.issn1005-0205.260303]
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参骨颗粒治疗绝经后骨质疏松的作用机制()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年03期
页码:
16-22
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Exploring the Mechanism of Shengu Granules in Treating Postmenopausal Osteoporosis
文章编号:
1005-0205(2026)03-0016-07
作者:
薛慧芝1; 曾家莹1; 陈晓聪1 姚楠2; 3; 李伟举1; 2; 陈慧龙1; 章雨童1; 吕朝晖2△
1广州中医药大学第五临床医学院(广州,510095); 2广东省第二中医院; 3广东省中医药研究开发重点实验室
Author(s):
XUE Huizhi1; ZENG Jiaying1; CHEN Xiaocong1; YAO Nan2; 3; LI Weiju1; 2; CHEN Huilong1; ZHANG Yutong1; LÜ Zhaohui2△
1The Fifth Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510095,China; 2Guangdong Provincial Second Hospital of Traditional Chinese Medicine,Guangzhou 510095,China; 3Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China.
关键词:
参骨颗粒;  肠-骨轴;  绝经后骨质疏松症;  肠道菌群;  炎症因子
Keywords:
Shengu granules;  gut-bone axis;  postmenopausal osteoporosis;  intestinal microbiota;  inflammatory factors
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260303
文献标志码:
A
摘要:
目的:探讨参骨颗粒对去卵巢大鼠肠道菌群和炎症因子的影响,阐述其治疗绝经后骨质疏松症的作用机制。方法:将36只雌性大鼠随机均分为假手术组、模型组和参骨颗粒组。参骨颗粒溶液灌胃12周后,Micro-CT测量分析右侧股骨远端骨密度和骨微结构; ELISA法检测血清中骨转换标志物Ⅰ型胶原氨基端前肽(PINP)、β-胶原特殊序列(β-CTX)及炎症因子白细胞介素-10(IL-10)、白细胞介素-17(IL-17)、转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α); Western Blot法检测胫骨组织中破骨细胞抑制因子和核因子κB受体活化因子配体(RANKL)的蛋白表达水平; 16S rRNA扩增及测序法检测肠道菌群。结果:与假手术组相比,参骨颗粒组大鼠的骨密度及BV/TV水平降低,Tb.Sp升高,结合骨微观结构,说明卵巢切除后大鼠骨结构呈骨质疏松样变。与模型组相比,参骨颗粒组大鼠PINP、IL-10、TGF-β及骨保护素(OPG)蛋白表达水平升高,β-CTX、IL-17、TNF-α及RANKL蛋白表达水平降低,差异均有统计学意义(P<0.05),说明参骨颗粒治疗可减少骨丢失,改善骨微结构。与模型组相比,参骨颗粒组大鼠肠道菌群的群落多样性提高、群落组成改善,差异有统计学意义(P<0.05),说明参骨颗粒可以减轻骨质疏松引起的肠道菌群失调。结论:参骨颗粒能够改善绝经后骨质疏松,其机制可能与肠道菌群和炎症因子介导的RANKL/OPG通路有关。
Abstract:
Objective:The effect of Shengu granules on intestinal microflora and inflammatory factors in ovariectomized rats was studied based on the gut-bone axis to reveal its mechanism in treating postmenopausal osteoporosis.Methods:Thirty-six female rats were randomly divided into three groups:Sham group,model group,and Shengu granules-treated group.After 12 weeks of oral gavage with Shengu granule solution,bone mineral density(BMD)and bone micros- tructures were measured by Micro-CT,and bone turnover markers(PINP,β-CTX)and inflammatory cytokines(IL-10,IL-17,TGF-β,TNF-α)were detected by ELISA.The protein expression levels of osteoprotegerin(OPG)and receptor activator of NF-κB ligand(RANKL)were detected by Western Blot,and the intestinal microbiota was analyzed by 16S rRNA amplification and sequencing.Results:Compared with the Sham group,the Shengu granules-treated group exhibited decreased BMD and BV/TV levels and increased Tb.Sp; combined with bone microstructure analysis,these findings indicate that ovariectomized rats developed osteoporotic-like bone changes.Compared with the model group,the Shengu granules-treated group showed significantly higher protein expression levels of PINP,IL-10,TGF-β,and OPG,as well as significantly lower expression levels of β-CTX,IL-17,TNF-α,and RANKL.All these differences were statistically significant(P<0.05),indicating that Shengu granules treatment reduces bone loss and improves bone microstructure in the Shengu granules-treated group.Furthermore,compared with the model group,the Shengu granules-treated group demonstrated increased gut microbiota community diversity and improved community composition(P<0.05),suggesting that Shengu granules can alleviate gut microbiota dysbiosis associated with osteoporosis.Conclusion:Shengu granules can improve postmenopausal osteoporosis,and the underlying mechanism may be related to the RANKL/OPG pathway mediated by gut microbiota and inflammatory factors.

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(收稿日期:2025-08-06)

备注/Memo

备注/Memo:
基金项目:广东省基础与应用基础研究基金项目(2025A1515010409)
广东省中医药局科研项目(20251020)
广东省第二中医院科研创新基金项目(SEZYY2023A08)
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更新日期/Last Update: 2026-03-15