[1]吴进,邱礼国,郭体康,等.苍柏丹防治骨科术后下肢深静脉血栓形成及炎症反应的实验研究[J].中国中医骨伤科杂志,2026,34(03):1-6.[doi:10.20085/j.cnki.issn1005-0205.260301]
　WU Jin,QIU Liguo,GUO Tikang,et al.Experimental Study on the Efficacy of Cangbai Dan in Treating Lower Extremity Deep Venous Thrombosis and Inflammatory Response after Orthopedic Surgery[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(03):1-6.[doi:10.20085/j.cnki.issn1005-0205.260301]
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苍柏丹防治骨科术后下肢深静脉血栓形成及炎症反应的实验研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年03期
页码:
1-6
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Experimental Study on the Efficacy of Cangbai Dan in Treating Lower Extremity Deep Venous Thrombosis and Inflammatory Response after Orthopedic Surgery
文章编号:
1005-0205(2026)03-0001-06
作者:
吴进1; 2; 邱礼国1; 2; 郭体康1; 2; 唐勇1; 2; 许晓彤1; 卢敏1; 邝高艳1; 谭开云1△
1湖南中医药大学第一附属医院(长沙,410007); 2湖南中医药大学
Author(s):
WU Jin1; 2; QIU Liguo1; 2; GUO Tikang1; 2; TANG Yong1; 2; XU Xiaotong1; LU Min1; KUANG Gaoyan1; TAN Kaiyun1△
1The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China; 2Hunan University of Chinese Medicine,Changsha 410208,China.
关键词:
苍柏丹;  骨科大手术;  下肢深静脉血栓;  炎症;  高迁移率族蛋白
Keywords:
Cangbai Dan;  major orthopedic surgery;  lower extremity deep vein thrombosis;  inflammation;  high-mobility group histones
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260301
文献标志码:
A
摘要:
目的:探讨苍柏丹对骨科大手术后下肢深静脉血栓形成及炎症的影响。方法:将50只大鼠随机分为空白组、模型组、苍柏丹低/高剂量组和阳性药物组,每组各10只,空白组正常喂养。除空白组外各组进行骨科大手术后大鼠下肢深静脉血栓模型造模,造模5 d后模型组和空白组大鼠开始生理盐水灌胃2次(2.5 mL/次),生理盐水0.3 mL皮下注射1次; 苍柏丹低剂量组大鼠临床1倍剂量中药(6.84 g/kg)灌胃2次,生理盐水0.3 mL皮下注射1次; 苍柏丹高剂量组大鼠临床2倍剂量中药(13.68 g/kg)灌胃2次,生理盐水0.3 mL皮下注射1次; 阳性药物组大鼠生理盐水灌胃2次(2.5 mL/次),低分子肝素钙注射液0.3 mL皮下注射1次。连续用药5 d,取大鼠大隐静脉1～2 cm石蜡包埋切片,苏木精-伊红(HE)染色法、免疫组化和Western Blot法检测各组血管内皮细胞内人核因子κB抑制蛋白α(NF-κB Inhibitor α,IκBα)和p65 蛋白表达; 腹主动脉采血用酶联免疫吸附测定法(ELISA)检测血浆白细胞介素(IL)-6、IL-8、高迁移率族蛋白B1(HMGB1)浓度。结果:大隐静脉HE染色可见苍柏丹和阳性药物对血管内膜发挥保护作用,高剂量组保护效果更好。ELISA检测各组大鼠血清IL-6、IL-8、HMGB1的表达水平,差异有统计学意义(P<0.05); 空白组<阳性药物组,苍柏丹高剂量组<苍柏丹低剂量组<模型组,苍柏丹高剂量组与阳性药物组比较差异无统计学意义(P>0.05)。Western Blot法检测IκBα和p65的蛋白表达:空白组<阳性药物组,苍柏丹高剂量组<苍柏丹低剂量组<模型组,差异有统计学意义(P<0.05); 苍柏丹高剂量组与阳性药物组比较差异无统计学意义(P>0.05)。免疫组化法检测IκBα和p65的蛋白表达:空白组<阳性药物组,苍柏丹高剂量组<苍柏丹低剂量组<模型组,差异有统计学意义(P<0.05); 苍柏丹高剂量组与阳性药物组比较差异无统计学意义(P>0.05)。结论:苍柏丹发挥其清热祛湿、活血祛瘀的功效,通过抑制NF-κB通路的异常活化,调控NF-κB p65、IκBα的mRNA和蛋白表达,进而有效抑制炎症反应,从而治疗下肢深静脉血栓形成。
Abstract:
Objective:To evaluate the effects of Cangbai Dan on deep vein thrombosis(DVT)formation and inflammation after major orthopedic surgery.Methods:Fifty rats were randomly assigned to five groups(n=10 each):blank,model,low-dose Cangbai Dan,high-dose Cangbai Dan,and positive drug group.Except for the blank group,all rats underwent DVT modeling.From day 5 post-surgery,rats received intragastric administration of Cangbai Dan(6.84 g/kg or 13.68 g/kg,twice daily),saline(2.5 mL,twice daily),or saline combined with low-molecular-weight heparin calcium(0.3 mL,subcutaneously,once daily)for five consecutive days.Histopathological changes in the great saphenous vein were evaluated by hematoxylin-eosin(HE)staining.Expression of IκBα and p65 proteins was assessed by immunohistochemistry and Western Blot.Serum IL-6,IL-8,and HMGB1 levels were measured by ELISA.Results:HE staining showed that Cangbai Dan and the positive drug protected the vascular endothelium,with superior effects in the high-dose group.ELISA demonstrated that IL-6,IL-8,and HMGB1 levels were lowest in the blank group and highest in the model group,with dose-dependent reductions in the Cangbai Dan groups.No significant difference was observed between the high-dose Cangbai Dan group and the positive drug group(P>0.05).Western Blot and immunohistochemistry showed consistent trends in IκBα and p65 expression.Conclusion:Cangbai Dan prevents postoperative lower limb DVT by inhibiting NF-κB pathway activation,downregulating NF-κB p65 and IκBα expression,and attenuating inflammatory responses.These findings support its therapeutic potential in the prevention of thrombosis after orthopedic surgery.

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(收稿日期:2025-06-20)

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(82274543)
湖南省自然科学基金项目(2023JJ30471)
湖南省中医药管理局项目(B2024046,D2024042)
湖南省教育厅优秀青年项目(24B0372)
湖南省卫计委课题(202204074679)
湖南省科技创新类湖湘青年英才项目(2024RC3203)
△通信作者 E-mail:tankaiyun0710@qq.com
更新日期/Last Update: 2026-03-15