[1]徐梅玲,张育珠,苏成龙.异槲皮苷对骨质疏松大鼠骨折愈合的影响[J].中国中医骨伤科杂志,2026,34(02):35-41.[doi:10.20085/j.cnki.issn1005-0205.260205]
　XU Meiling,ZHANG Yuzhu,SU Chenglong.The Effect of Isoquercitrin on Fracture Healing Inosteoporotic Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(02):35-41.[doi:10.20085/j.cnki.issn1005-0205.260205]
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异槲皮苷对骨质疏松大鼠骨折愈合的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年02期
页码:
35-41
栏目:
基础研究
出版日期:
2026-02-15

文章信息/Info

Title:
The Effect of Isoquercitrin on Fracture Healing Inosteoporotic Rats
文章编号:
1005-0205(2026)02-0035-07
作者:
徐梅玲1; 张育珠2; 苏成龙2
1青海省第五人民医院(西宁,810006);2西宁市第一人民医院
Author(s):
XU Meiling1; ZHANG Yuzhu2; SU Chenglong2
1The Fifth People's Hospital of Qinghai Province,Xining 810006,China; 2Xining First People's Hospital,Xining 810002,China.
关键词:
异槲皮苷;  Wnt家族成员3a/低密度脂蛋白受体相关蛋白5(Wnt3a/Lrp5);  骨质疏松;  骨折愈合
Keywords:
isoquercitrin;  Wnt family member 3a/low-density lipoprotein receptor-related protein 5(Wnt3a/Lrp5);  osteoporosis;  fracture healing
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260205
文献标志码:
A
摘要:
目的:探究异槲皮苷(IQ)调节Wnt家族成员3a(Wnt3a)/低密度脂蛋白受体相关蛋白5(Lrp5)信号通路对骨质疏松症(OP)大鼠骨折愈合的影响。方法:采用双侧卵巢切除术及右侧股骨干骨折髓内固定诱导建立骨质疏松症大鼠骨折模型。将骨质疏松症模型建立成功的大鼠随机分为模型组组、L-IQ组、M-IQ组、H-IQ组(分别灌胃12.5,25,50 mg/kg的异槲皮苷)、DKK1组(灌胃50 mg/kg的异槲皮苷+腹腔注射100 mg/kg的Wnt3a/Lrp5信号通路抑制剂DKK1),每组各10只。另外取10只正常大鼠为对照组(其余步骤与造模组相同)。摄X线片检查大鼠股骨干并观察骨折愈合情况; 用骨密度(BMD)扫描仪检测大鼠股骨骨折端的骨密度; ELISA检测大鼠血清中骨钙素(OCN)、骨源性碱性磷酸酶(BALP)、Ⅰ型前胶原羧基端肽(PICP)的表达水平; HE染色观察股骨骨折处的病理学变化; 用三点弯曲试验和压缩试验对大鼠股骨骨生物学进行检测; Western Blot法检测大鼠骨折部位Wnt3a、Lrp5蛋白的表达水平。结果:与对照组相比,模型组大鼠的股骨存在清晰的骨折线,未完全愈合,骨小梁细、结构疏松紊乱,骨折愈合评分、股骨骨密度、最大位移、最大应力、最大负荷,血清中骨钙素、碱性磷酸酶、Ⅰ型前胶原羧基端肽以及Wnt3a、Lrp5表达水平降低,差异有统计学意义(P<0.05); 与模型组相比,L-IQ组、M-IQ组、H-IQ组大鼠骨折愈合较好、骨折线模糊、骨小梁变粗、结构整齐致密,骨折愈合评分、股骨骨密度、最大位移、最大应力、最大负荷,血清中骨钙素、碱性磷酸酶、Ⅰ型前胶原羧基端肽以及Wnt3a、Lrp5表达水平显著升高,差异有统计学意义(P<0.05); 与H-IQ组相比,DKK1组大鼠骨折愈合较差、骨折线清晰、骨小梁结构变细、结构疏松紊乱,骨折愈合评分、股骨骨密度、最大位移、最大应力、最大负荷,血清中骨钙素、碱性磷酸酶、Ⅰ型前胶原羧基端肽以及Wnt3a、Lrp5表达水平降低,差异有统计学意义(P<0.05)。结论:异槲皮苷可能通过激活Wnt3a/Lrp5信号通路,改善骨小梁病理改变,有利于骨质疏松症大鼠骨折的愈合。
Abstract:
Objective:To investigate the effect of isoquercitrin(IQ)on fracture healing in osteoporotic(OP)rats by adjusting Wnt family member 3a(Wnt3a)/low-density lipoprotein receptor-related protein 5(Lrp5)signaling pathway.Methods:In the study,the OP rat fracture model was established through bilateral oophorectomy and intramedullary fixation of the right femoral shaft fracture.The OP model rats were divided into model group,L-IQ group,M-IQ group,H-IQ group(administered 12.5,25,and 50 mg/kg IQ by gavage),and DKK1 group(administered 50 mg/kg IQ by gavage combined with intraperitoneal injection of 100 mg/kg Wnt3a/Lrp5 signaling pathway inhibitor DKK1),with 10 rats in each group.In addition,10 normal rats were designated as control group(the rest of the steps were the same as the model group).The control group and the model group were given an equal amount of physiological saline,once a day for 4 consecutive weeks.X-ray imaging was used to examine femoral shaft fractures in rats and evaluate fracture healing.The bone mineral density(BMD)scanner was used to detect the BMD of the fractured end of the femur in rats.ELISA was used to measure osteocalcin(OCN),bone alkaline phosphatase(BALP),and type I procollagen carboxy-terminal peptide(PICP)in serum.HE staining was used to observe the pathological changes at the site of femoral fractures.Three-point bending experiment and the compression experiment were used to detect the biology of rat femur bone.Western Blot was used to detect Wnt3a and Lrp5 proteins at the fracture site of rats.Results:Compared with the control group,the model group had clear fracture lines in the femurs of rats,incomplete healing,thinner bone trabeculae,reduced numbers,increased gaps,and a loose and disordered structure.The fracture healing score,femur BMD,maximum displacement,maximum stress,maximum load,serum OCN,BALP,PICP,and Wnt3a,Lrp5 reduced(P<0.05).For the model group,the L-IQ group,M-IQ group,and H-IQ group showed better fracture healing,blurred fracture lines,thickened bone trabeculae,raised quantity,reduced gaps,and neat,dense structures in rats.The fracture healing score,femoral BMD,maximum displacement,maximum stress,maximum load,serum OCN,BALP,PICP,and Wnt3a,Lrp5 were greatly increased(P<0.05).For H-IQ group,the DKK1 group had poorer fracture healing,clearer fracture lines,thinner bone trabeculae,reduced numbers,smaller gaps,and looser,disordered structures in rats.The fracture healing score,femoral BMD,maximum displacement,maximum stress,maximum load,serum OCN,BALP,PICP,Wnt3a,and Lrp5 were all reduced(P<0.05).Conclusion:IQ may promote fracture healing in osteoporotic rats by activating the Wnt3a/Lrp5 signaling pathway.

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(收稿日期:2025-06-19)

更新日期/Last Update: 2026-02-15