[1]王贺,刘佳,刘畅.山姜素抑制类风湿关节炎大鼠关节滑膜血管生成的机制研究[J].中国中医骨伤科杂志,2026,34(02):28-34.[doi:10.20085/j.cnki.issn1005-0205.260204]
　WANG He,LIU Jia,LIU Chang.Mechanistic Study on Alpinetin-Mediated Suppression of Synovial Angiogenesis in Rheumatoid Arthritis Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(02):28-34.[doi:10.20085/j.cnki.issn1005-0205.260204]
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山姜素抑制类风湿关节炎大鼠关节滑膜血管生成的机制研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年02期
页码:
28-34
栏目:
基础研究
出版日期:
2026-02-15

文章信息/Info

Title:
Mechanistic Study on Alpinetin-Mediated Suppression of Synovial Angiogenesis in Rheumatoid Arthritis Rats
文章编号:
1005-0205(2026)02-0028-07
作者:
王贺1; 刘佳1; 刘畅1
1唐山市中心医院(河北 唐山,063000)
Author(s):
WANG He1; LIU Jia1; LIU Chang1
1Tangshan Central Hospital,Tangshan 063000,Hebei China.
关键词:
山姜素;  缺氧诱导因子-1α/血管内皮生长因子通路;  类风湿关节炎;  血管生成
Keywords:
alpinetin;  hypoxia-inducible factor-1α/vascular endothelial growth factor pathway;  rheumatoid arthritis;  angiogenesis
分类号:
R593.22; R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260204
文献标志码:
A
摘要:
目的:探究山姜素调节缺氧诱导因子-1α/血管内皮生长因子(HIF-1α/VEGF)通路对类风湿关节炎(RA)大鼠血管生成的影响。方法:采用Ⅱ型胶原蛋白诱导法构建类风湿关节炎大鼠模型,将大鼠分为对照组、关节炎组、山姜素组、泼尼松龙组和山姜素+氯化钴组,检测各组大鼠关节炎指数、关节肿胀程度及体重以分析临床特征; ELISA测定各组大鼠血清中IL-17、IL-6和IL-1β含量; HE染色试剂和番红O固绿进行踝关节病理特征分析; 免疫组织化学(IHC)和免疫组织荧光(IHF)检测血管生成数量; Western Blot法检测滑膜组织中HIF-1α、VEGF、VEGFR、CD31蛋白水平。结果:与对照组比较,关节炎组大鼠关节炎指数、关节肿胀程度、IL-17含量、IL-6含量、IL-1β含量、踝关节HE评分、番红O评分、CD31阳性表达量、新生血管数量、成熟血管数量、总血管数量、HIF-1α蛋白水平、VEGF蛋白水平、VEGFR蛋白水平、CD31蛋白水平均上调,体重下降,差异有统计学意义(P<0.05); 与关节炎组比较,山姜素组和泼尼松龙组大鼠关节炎指数、关节肿胀程度、IL-17含量、IL-6含量、IL-1β含量、踝关节HE评分、番红O评分、CD31阳性表达量、新生血管数量、HIF-1α蛋白水平、VEGF蛋白水平、VEGFR蛋白水平、CD31蛋白水平均下调,体重上升,差异有统计学意义(P<0.05); 与山姜素组比较,山姜素+氯化钴组大鼠关节炎指数、关节肿胀程度、IL-17含量、IL-6含量、IL-1β含量、踝关节HE评分、番红O评分、CD31阳性表达量、新生血管数量、HIF-1α蛋白水平、VEGF蛋白水平、VEGFR蛋白水平、CD31蛋白水平均上调,体重下降,差异有统计学意义(P<0.05),泼尼松龙组大鼠上述指标变化差异无统计学意义(P>0.05)。结论:山姜素抑制HIF-1α/VEGF通路,缓解类风湿关节炎大鼠血管生成。
Abstract:
Objective:To investigate the effect of alpinetin on angiogenesis in rheumatoid arthritis(RA)rats by adjusting hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)pathway.Methods:The RA rat model was constructed using type II collagen induction method.The rats were separated into control group,arthritis model group,alpinetin treatment group,prednisolone treatment group,and alpinetin +cobalt chloride group.The arthritis index,joint swelling,and body weight of rats were measured to analyze clinical features.ELISA was used to measure the serum IL-17,IL-6,and IL-1β of rats in each group.HE staining reagent and safranin O-green were used to discuss the pathological features of ankle joints.Immunohistochemistry(IHC)and immunohistochemistry fluorescence(IHF)were used to detect the quantity of angiogenesis.Western Blot was used to detect the HIF-1α,VEGF,VEGFR,and CD31 proteins in synovial tissue.Results:Compared with the control group,the arthritis model group showed upregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safranin O score,CD31 positivity,number of new blood vessels,number of mature blood vessels,total blood vessels,HIF-1α protein,VEGF protein,VEGFR protein,and CD31 protein,while downregulation of weight(P<0.05).Compared with the arthritis model group,the alpinetin treatment group and prednisolone treatment group showed downregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safranin O score,CD31 positivity,number of new blood vessels,HIF-1α protein,VEGF protein,VEGFR protein,and CD31 protein,while upregulation of weight(P<0.05).Compared with the alpinetin treatment group,the alpinetin + cobalt chloride group showed upregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safranin O score,CD31 positivity,number of new blood vessels,HIF-1α protein,VEGF protein,VEGFR protein,and CD31 protein,while downregulation of weight(P<0.05).The above indicators in the prednisolone treatment group were not prominently different(P>0.05).Conclusion:Alpinetin inhibits the HIF-1α/VEGF pathway and alleviates angiogenesis in RA rats.

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(收稿日期:2025-06-11)

备注/Memo

备注/Memo:
基金项目:河北省医学科学研究重点课题计划项目(20231838)
更新日期/Last Update: 2026-02-15