[1]刘衡,苏红青,杨艳兵,等.高良姜素对大鼠骨髓间充质干细胞成骨分化的影响[J].中国中医骨伤科杂志,2026,34(02):22-27.[doi:10.20085/j.cnki.issn1005-0205.260203]
　LIU Heng,SU Hongqing,YANG Yanbing,et al.The Effect of Galangin on the Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(02):22-27.[doi:10.20085/j.cnki.issn1005-0205.260203]
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高良姜素对大鼠骨髓间充质干细胞成骨分化的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年02期
页码:
22-27
栏目:
基础研究
出版日期:
2026-02-15

文章信息/Info

Title:
The Effect of Galangin on the Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
文章编号:
1005-0205(2026)02-0022-06
作者:
刘衡1; 苏红青1; 杨艳兵1; 张玉宝1; 季向南1
1衡水市第二人民医院(河北 衡水,053000)
Author(s):
LIU Heng1; SU Hongqing1; YANG Yanbing1; ZHANG Yubao1; JI Xiangnan1
1The Second People's Hospital of Hengshui,Hengshui 053000,Hebei China.
关键词:
高良姜素;  大鼠;  骨髓间充质干细胞;  成骨分化;  BMP2/RUNX2通路
Keywords:
galangin;  rat;  bone marrow mesenchymal stem cells;  osteogenic differentiation;  BMP2/RUNX2 pathway
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260203
文献标志码:
A
摘要:
目的:探究高良姜素对大鼠骨髓间充质干细胞(BMSCs)成骨分化的影响及其作用机制。方法:构建骨质疏松症(OP)大鼠模型并将其分为对照组、模型组、高良姜素低剂量组、高良姜素高剂量组。Micro-CT检测大鼠股骨微结构、qRT-PCR检测股骨组织成骨相关基因(ALP、RUNX2、OPN、BMP2)的相对表达水平。从骨质疏松症大鼠股骨中提取骨髓间充质干细胞并将其分为对照组、高良姜素低剂量组、高良姜素高剂量组、Noggin组、高良姜素高剂量+Noggin组。用CCK-8试剂盒检测细胞活力,用碱性磷酸酶试剂盒检测细胞上清碱性磷酸酶水平,用茜素红染色法检测骨髓间充质干细胞的成骨分化能力; 用Western Blot法检测各组骨髓间充质干细胞中BMP2/RUNX2通路有关蛋白表达量。结果:随着高良姜素剂量的提高,骨质疏松症大鼠骨密度(BMD)、BV/TV、Tb.N、Tb.Th指标水平明显升高,差异有统计学意义(P<0.05)。从骨质疏松症大鼠股骨组织提取的第3代骨髓间充质干细胞生长良好,成骨诱导显示有矿化结节,说明骨髓间充质干细胞提取成功。与对照组相比,随着高良姜素剂量的提高,骨髓间充质干细胞活性增强,细胞上清碱性磷酸酶含量增加,钙化结节增多,BMP2、RUNX2、ALP、OPN、Collagen I蛋白表达水平明显增加,差异有统计学意义(P<0.05); 抑制BMP2/RUNX2通路可明显降低骨髓间充质干细胞活性和细胞上清碱性磷酸酶水平,同时矿化结节数量减少,BMP2、RUNX2、ALP、OPN、Collagen I蛋白表达水平降低,差异有统计学意义(P<0.05); 与高良姜素高剂量组相比,高良姜素高剂量+Noggin组骨髓间充质干细胞活性和细胞上清碱性磷酸酶水平降低,矿化结节数量减少,BMP2、RUNX2、ALP、OPN、Collagen I蛋白表达水平明显减少,差异有统计学意义(P<0.05)。结论:高良姜素可提高骨质疏松症大鼠骨强度,增强骨髓间充质干细胞活性并促进其成骨分化,其潜在机制可能与BMP2/RUNX2通路的激活有关。
Abstract:
Objective:To explore the effect of galangin on osteogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs)and its mechanism of action.Methods:Osteoporosis(OP)model rats were constructed and assigned to control group,model group,low-dose galangin group,and high-dose galangin group.Micro-CT was used to detect the microstructure of the rat femur,and qRT-PCR was used to detect the relative expression levels of osteogenic-related genes(ALP,RUNX2,OPN,BMP2)in femoral tissue.Bone marrow mesenchymal stem cells(BMSCs)were extracted from the femurs of OP rats and divided into the control group,the low-dose galangin group,the high-dose galangin group,the Noggin group,and the high-dose galangin +Noggin group.CCK-8 assay kit was used to detect cell viability.The ALP assay kit was used to detect ALP in the cell supernatant.The Alizarin Red staining method was performed to detect the osteogenic differentiation ability of BMSCs.Western Blot was performed to detect the BMP2/RUNX2 pathway-related proteins in BMSCs.Results:With the increase of galangin dose,the bone mineral density(BMD),BV/TV,Tb.N,and Tb.Th in OP rats significantly increased(P<0.05).The third-generatio generation BMSCs isolated from femoral tissue of OP rats exhibited good growth,and osteogenic induction resulted in the formation of mineralized nodules,indicating successful extraction of BMSCs.For the control group,as the galangin concentration increased,the activity of BMSCs increased,the ALP level in the cell supernatant increased,the calcified nodules increased,the BMP2,RUNX2,ALP,OPN,and Collagen I proteins significantly increased(P<0.05).Inhibition of the BMP2/RUNX2 pathway clearly reduced the activity of BMSCs and ALP in cell supernatant,reduced mineralized nodules,and decreased BMP2,RUNX2,ALP,OPN,and Collagen I proteins(P<0.05).For the high-dose galangin group,the high-dose galangin+Noggin group showed decreased BMSCs activity and ALP in the cell supernatant,reduced mineralized nodules,and clearly reduced BMP2,RUNX2,ALP,OPN,and Collagen I proteins(P<0.05).Conclusion:Galangin can improve bone strength,enhance BMSCs activity,and promote osteogenic differentiation in OP rats,and its potential mechanism may be related to the activation of the BMP2/RUNX2 pathway.

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(收稿日期:2025-07-11)

备注/Memo

备注/Memo:
基金项目:衡水市科技计划项目(2022014079Z)
更新日期/Last Update: 2026-02-15