[1]刘庆庆,郭翔云,秦金然,等.代谢组学驱动的补骨生髓方改善铁蓄积型骨质疏松症的作用机制研究[J].中国中医骨伤科杂志,2026,34(01):84-91.[doi:10.20085/j.cnki.issn1005-0205.260112]
　LIU Qingqing,GUO Xiangyun,QIN Jinran,et al.Metabolomics-Driven Investigation of the Mechanism by Bugu Shengsui Decoction Ameliorates Iron Overload-Induced Osteoporosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(01):84-91.[doi:10.20085/j.cnki.issn1005-0205.260112]
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代谢组学驱动的补骨生髓方改善铁蓄积型骨质疏松症的作用机制研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年01期
页码:
84-91
栏目:
骨质疏松症
出版日期:
2026-01-15

文章信息/Info

Title:
Metabolomics-Driven Investigation of the Mechanism by Bugu Shengsui Decoction Ameliorates Iron Overload-Induced Osteoporosis
文章编号:
1005-0205(2026)01-0084-08
作者:
刘庆庆1; 郭翔云1; 秦金然1; 王亮1; 齐保玉2; 李琰2; 谢雁鸣3; 魏戌2△; 章轶立1△
1南京中医药大学中西医结合学院(南京,210023)
2中国中医科学院望京医院
3中国中医科学院中医临床基础医学研究所
Author(s):
LIU Qingqing1; GUO Xiangyun1; QIN Jinran1; WANG Liang1; QI Baoyu2; LI Yan2; XIE Yanming3; WEI Xu2△; ZHANG Yili1△
1School of Integrated Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China; 2Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China; 3Institute of Clinical Basic Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China.
关键词:
绝经后骨质疏松;  补骨生髓方;  铁蓄积;  铁蓄积型骨质疏松症;  代谢组学;  甘油磷脂代谢
Keywords:
postmenopausal osteoporosis;  Bugu Shengsui decoction;  iron accumulation;  iron overload-induced osteoporosis;  metabolomics;  glycerophospholipid metabolism
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260112
文献标志码:
A
摘要:
目的:探讨补骨生髓方改善铁蓄积型骨质疏松症的作用机制,重点分析其对骨代谢和铁代谢的调控作用,并基于代谢组学揭示关键代谢通路。方法:构建“去卵巢+铁蓄积”复合大鼠模型,模拟铁蓄积型骨质疏松症,设置假手术组、去卵巢组、模型组、阳性药组及补骨生髓方高/低剂量组,共6组。Micro-CT检测股骨骨密度及骨微结构参数,苏木精-伊红(HE)染色观察骨组织形态,ELISA检测血清骨代谢指标; 普鲁士蓝染色评估组织铁沉积,检测血清铁蛋白、血清铁及组织总铁含量。采用UPLC-Q-Exactive Orbitrap MS技术对假手术组、模型组及补骨生髓方高剂量组胫骨组织进行非靶向代谢组学分析,按P<0.05、倍数变化(FC)≥1.2或≤1/1.2、变量权重值(VIP)>1.0的标准筛选差异代谢物,进行KEGG通路富集分析及相关性分析。结果:与模型组相比,补骨生髓方高剂量组股骨骨密度显著升高(P<0.05),骨小梁微结构明显改善(BV/TV、BS/TV、Tb.N升高,Tb.Sp降低,P<0.05),回调骨代谢指标异常升高(P<0.05); 显著降低肝脏及股骨组织铁含量、血清铁蛋白和血清铁水平(P<0.05)。代谢组学分析鉴定出135个共同差异代谢物,其中87个在病理状态下下调、干预后上调; KEGG富集分析显示甘油磷脂代谢为核心调控通路,关键代谢物PC(22:6/0:0)和PC(18:1/15:0)与股骨骨密度显著正相关(r=0.445 6,0.380 2,P<0.05)。结论:补骨生髓方通过靶向甘油磷脂代谢通路及磷脂酰胆碱,协同调节骨代谢与铁代谢,从而改善铁蓄积型骨质疏松症,为中医药防治该病提供了实验依据。
Abstract:
Objective:To investigate the mechanism by Bugu Shengsui decoction(BGSSD)ameliorates iron overload-induced osteoporosis,with particular focus on its regulatory effects on bone and iron metabolism,and to elucidate the key metabolic pathways through metabolomics analysis.Methods:A composite rat model of “ovariectomy+iron overload” was established to simulate iron overload-induced osteoporosis.Six groups were established:the sham-operated group,the ovariectomized group,the model group,the positive drug group,and the BGSSD high-dose and low-dose groups.Micro-CT was employed to assess femoral bone mineral density(BMD)and bone microarchitecture parameters.HE staining was performed to observe bone tissue morphology,and ELISA was used to measure serum bone metabolism markers.Prussian blue staining was conducted to evaluate tissue iron deposition,and serum ferritin,serum iron,and total tissue iron content were measured.Untargeted metabolomics analysis of tibial tissue from the sham-operated,model,and BGSSD high-dose groups was performed using UPLC-Q-Exactive Orbitrap MS technology.Differential metabolites were screened according to the criteria of P<0.05,fold change(FC)≥1.2 or ≤1/1.2,and variable importance in projection(VIP)>1.0,followed by KEGG pathway enrichment analysis and correlation analysis.Results:Compared with the model group,the BGSSD high-dose group exhibited significantly increased femoral BMD(P<0.05),markedly improved trabecular bone microstructure(elevated BV/TV,BS/TV,and Tb.N; reduced Tb.Sp; P<0.05),and restoration of abnormally elevated bone metabolism markers(P<0.05).Furthermore,iron content in liver and femoral tissue,as well as serum ferritin and serum iron levels,was significantly reduced(P<0.05).Metabolomics analysis identified 135 common differential metabolites,of which 87 were downregulated in the pathological state and upregulated following intervention.KEGG enrichment analysis revealed glycerophospholipid metabolism as the core regulatory pathway,with key metabolites PC(22:6/0:0)and PC(18:1/15:0)showing significant positive correlations with femoral BMD(r=0.445 6,0.380 2; P<0.05).Conclusion:BGSSD ameliorates iron overload-induced osteoporosis by targeting the glycerophospholipid metabolic pathway and phosphatidylcholine to synergistically regulate bone and iron metabolism,providing experimental evidence for the prevention and treatment of this disease with traditional Chinese medicine.

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(收稿日期:2025-11-10)

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(82205140)
江苏省科技计划专项资金项目(基础研究计划自然科学基金)(BK20220468)
江苏省研究生科研与实践创新计划项目(KYCX25_2355)
△通信作者 E-mail:zhangyili823@126.com(章轶立)
weixu.007@163.com(魏戌)
更新日期/Last Update: 2026-01-15