[1]聂颖,段嘉豪,杨少锋,等.补阳还五汤通过抑制JAK2/STAT3通路促进BV2小胶质细胞M2极化以减轻炎症反应[J].中国中医骨伤科杂志,2026,34(01):43-50.[doi:10.20085/j.cnki.issn1005-0205.260107]
　NIE Ying,DUAN Jiahao,YANG Shaofeng,et al.Buyang Huanwu Decoction Promotes M2 Polarization of BV2 Microglia by Inhibiting the JAK2/STAT3 Pathway to Alleviate Inflammatory Response[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(01):43-50.[doi:10.20085/j.cnki.issn1005-0205.260107]
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补阳还五汤通过抑制JAK2/STAT3通路促进BV2小胶质细胞M2极化以减轻炎症反应()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年01期
页码:
43-50
栏目:
脊柱脊髓疾病
出版日期:
2026-01-15

文章信息/Info

Title:
Buyang Huanwu Decoction Promotes M2 Polarization of BV2 Microglia by Inhibiting the JAK2/STAT3 Pathway to Alleviate Inflammatory Response
文章编号:
1005-0205(2026)01-0043-08
作者:
聂颖1; 段嘉豪1; 杨少锋1; 陈龙1; 李兆勇1; 郭彦涛1; 范瑜洁2△; 杨雷1△
1湖南中医药大学第一附属医院(长沙,410007)
2湖南省第二人民医院
Author(s):
NIE Ying1; DUAN Jiahao1; YANG Shaofeng1; CHEN Long1; LI Zhaoyong1; GUO Yantao1; FAN Yujie2△; YANG Lei1△
1The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China; 2The Second People's Hospital of Hunan Province,Changsha 410007,China.
关键词:
补阳还五汤;  脊髓损伤;  BV2小胶质细胞;  M2极化;  神经炎症;  JAK2/STAT3信号通路
Keywords:
Buyang Huanwu decoction;  spinal cord injury;  BV2 microglia;  M2 polarization;  neuroinflammation;  JAK2/STAT3 signaling pathway
分类号:
R285.6
DOI:
10.20085/j.cnki.issn1005-0205.260107
文献标志码:
A
摘要:
目的:探讨补阳还五汤(BYHWD)调节JAK2/STAT3信号通路对脂多糖(LPS)中BV2小胶质细胞极化的影响。方法:建立BV2小胶质细胞脂多糖诱导神经炎症损伤模型,将细胞分为空白组、模型组、BYHWD组、Colivelin组(STAT3激活剂)及BYHWD+Colivelin组。用细胞计数试剂盒-8(CCK-8)法检测细胞活力; 酶联免疫吸附(ELISA)测定肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-4(IL-4)的分泌水平; 免疫荧光共表达F4/80/iNOS、F4/80/CD206观察BV2细胞活化情况; 免疫印迹法检测iNOS、IL-1β、Arg1、Fizz1和JAK2/STAT3信号通路相关蛋白的表达情况。结果:与空白组比较,模型组中细胞活力,IL-10、IL-4炎症因子水平,F4/80和CD206双阳性细胞比例,Fizz1、Arg1蛋白表达水平显著下调(P<0.05); IL-6、TNF-α炎症因子水平,F4/80和iNOS双阳性细胞比例,iNOS、IL-1β、p-JAK2/JAK2和p-STAT3/STAT3蛋白表达水平显著上调(P<0.05)。与模型组比较,BYHWD组细胞活力,IL-10、IL-4炎症因子水平,F4/80和CD206双阳性细胞比例,Fizz1、Arg1蛋白表达水平显著上调(P<0.05); IL-6、TNF-α炎症因子水平,F4/80和iNOS双阳性细胞比例,iNOS、IL-1β、p-JAK2/JAK2和p-STAT3/STAT3蛋白表达水平显著下调(P<0.05)。与BYHWD组比较,Colivelin组细胞活力,IL-10、IL-4炎症因子水平,F4/80和CD206双阳性细胞比例,Fizz1、Arg1蛋白表达水平显著下调(P<0.05); IL-6、TNF-α炎症因子水平,F4/80和iNOS双阳性细胞比例,iNOS、IL-1β、p-JAK2/JAK2和p-STAT3/STAT3蛋白表达水平显著上调(P<0.05)。与Colivelin组比较,BYHWD+Colivelin组细胞活力,IL-10、IL-4炎症因子水平,F4/80和CD206双阳性细胞比例,Fizz1、Arg1蛋白表达水平显著上调(P<0.05); IL-6、TNF-α炎症因子水平,F4/80和iNOS双阳性细胞比例,iNOS、IL-1β、p-JAK2/JAK2和p-STAT3/STAT3蛋白表达水平显著下调(P<0.05)。结论:补阳还五汤可能通过抑制JAK2/STAT3信号通路激活,减少脂多糖诱导的BV2小胶质细胞M1型活化,促进其向M2型极化,为临床治疗脊髓损伤后神经炎症提供了实验依据。
Abstract:
Objective:To investigate the effect of Buyang Huanwu decoction(BYHWD)on the polarization of BV2 microglial cells induced by lipopolysaccharide(LPS)by regulating the JAK2/STAT3 signaling pathway.Methods:A neuroinflammatory injury model was established in BV2 microglial cells using LPS.The cells were divided into blank group,model group,BYHWD group,Colivelin group(STAT3 activator),and BYHWD + Colivelin group.Cell viability was assessed using the Cell Counting Kit-8(CCK-8)assay.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the secretion levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10),and interleukin-4(IL-4).Immunofluorescence co-expression analysis of F4/80/iNOS and F4/80/CD206 was used to observe BV2 cell activation.Western Blot was performed to detect the expression of iNOS,IL-1β,Arg1,Fizz1,and proteins related to the JAK2/STAT3 signaling pathway.Results:Compared with the blank group,the model group exhibited significantly decreased cell viability,levels of the anti-inflammatory cytokines IL-10 and IL-4,the proportion of F4/80 and CD206 double-positive cells,and the protein expression levels of Fizz1 and Arg1(P<0.05).Conversely,the levels of the pro-inflammatory cytokines IL-6 and TNF-α,the proportion of F4/80 and iNOS double-positive cells,and the protein expression levels of iNOS,IL-1β,p-JAK2/JAK2,and p-STAT3/STAT3 were significantly increased(P<0.05).Compared with the model group,the BYHWD group showed significantly increased cell viability,levels of IL-10 and IL-4,the proportion of F4/80 and CD206 double-positive cells,and the protein expression levels of Fizz1 and Arg1(P<0.05),while the levels of IL-6 and TNF-α,the proportion of F4/80 and iNOS double-positive cells,and the protein expression levels of iNOS,IL-1β,p-JAK2/JAK2,and p-STAT3/STAT3 were significantly decreased(P<0.05).Compared with the BYHWD group,the Colivelin group exhibited significantly decreased cell viability,levels of IL-10 and IL-4,the proportion of F4/80 and CD206 double-positive cells,and the protein expression levels of Fizz1 and Arg1(P<0.05),along with significantly increased levels of IL-6 and TNF-α,the proportion of F4/80 and iNOS double-positive cells,and the protein expression levels of iNOS,IL-1β,p-JAK2/JAK2,and p-STAT3/STAT3(P<0.05).Compared with the Colivelin group,the BYHWD + Colivelin group showed significantly increased cell viability,levels of IL-10 and IL-4,the proportion of F4/80 and CD206 double-positive cells,and the protein expression levels of Fizz1 and Arg1(P<0.05),while the levels of IL-6 and TNF-α,the proportion of F4/80 and iNOS double-positive cells,and the protein expression levels of iNOS,IL-1β,p-JAK2/JAK2,and p-STAT3/STAT3 were significantly decreased(P<0.05).Conclusion:BYHWD may inhibit the activation of the JAK2/STAT3 signaling pathway,thereby reducing LPS-induced M1-type activation of BV2 microglia and promoting their polarization toward the M2 phenotype.This provides experimental evidence for the clinical treatment of neuroinflammation following spinal cord injury(SCI).

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(收稿日期:2025-11-15)

备注/Memo

备注/Memo:
基金项目:湖南省自然科学基金(2025JJ90030)
湖南省教育厅重点项目(22A0261)
湖南省中医药管理局项目(B2023028)
△通信作者 E-mail:1169949914@qq.com(杨雷)
18593532@qq.com(范瑜洁)
更新日期/Last Update: 2026-01-15