[1]尹逊路,杨克新,高春雨,等.壮腰通络方调节SIRT1/HIF-1α信号通路抑制大鼠髓核细胞衰老延缓椎间盘退变的实验研究[J].中国中医骨伤科杂志,2026,34(01):35-42.[doi:10.20085/j.cnki.issn1005-0205.260106]
　WEI Jiaming,ZHAO Zelong,SUN Kai,et al.Zhuangyao Tongluo Formula Delays Intervertebral Disc Degeneration by Inhibiting Rat Nucleus Pulposus Cell Senescence via the SIRT1/HIF-1α Signaling Pathway[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(01):35-42.[doi:10.20085/j.cnki.issn1005-0205.260106]
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壮腰通络方调节SIRT1/HIF-1α信号通路抑制大鼠髓核细胞衰老延缓椎间盘退变的实验研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年01期
页码:
35-42
栏目:
脊柱脊髓疾病
出版日期:
2026-01-15

文章信息/Info

Title:
Zhuangyao Tongluo Formula Delays Intervertebral Disc Degeneration by Inhibiting Rat Nucleus Pulposus Cell Senescence via the SIRT1/HIF-1α Signaling Pathway
文章编号:
1005-0205(2026)01-0035-08
作者:
尹逊路1; 2; 杨克新1; 2; 高春雨1; 2; 彭伟4; 朱立国1; 2
1中国中医科学院望京医院(北京,100102 )
2数智中医防治骨与关节退行性疾病北京市重点实验室
3北京按摩医院
4中国中医科学院西苑医院
Author(s):
WEI Jiaming1; 2; ZHAO Zelong3; SUN Kai1; 2△; QIN Xiaokuan1; 2; WEI Xu1; 2; LI Linghui1; 2; YIN Xunlu1; 2; YANG Kexin1; 2; GAO Chunyu1; 2; PENG Wei4; ZHU Liguo1; 2
1Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China; 2Beijing Key Laboratory of Digital Intelligence TCM for Prevention and Treatment of Degenerative Bone and Joint Diseases,Beijing 100102,China; 3Beijing Massage Hospital,Beijing 100035,China; 4Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing 100091,China.
关键词:
壮腰通络方;  椎间盘退变;  衰老;  氧化应激
Keywords:
Zhuangyao Tongluo formula;  intervertebral disc degeneration;  senescence;  oxidative stress
分类号:
R681.5
DOI:
10.20085/j.cnki.issn1005-0205.260106
文献标志码:
A
摘要:
目的:基于补肾活血理论,探究壮腰通络方能否通过调节SIRT1/HIF-1α信号通路抑制椎间盘退变大鼠髓核细胞衰老,从而发挥延缓腰椎间盘退变的作用机制。方法:将70只8周龄SPF级雄性大鼠随机分为6组:空白组、模型组、阳性药对照组(塞来昔布)、壮腰通络方低、中、高剂量组。除空白组外,其余各组采用切除棘突、关节突及棘上韧带的方法建立腰椎失稳导致腰椎间盘退变大鼠模型。造模后第3天开始灌胃给药,连续干预8周,观察各组大鼠行为学表现,采集椎间盘及髓核组织。采用苏木精-伊红(HE)染色和番红O-固绿染色(SO/FG)观察椎间盘组织形态; 采用蛋白质免疫印迹试验(Western Blot)和实时荧光定量PCR(RT-qPCR)检测髓核组织中SIRT1、HIF-1α、p16及Ki-67的蛋白和mRNA表达水平。结果:与空白组相比,模型组大鼠髓核组织皱缩聚集,纤维环排列杂乱,基质蛋白聚糖丢失,HIF-1α、p16蛋白及mRNA表达水平升高,差异有统计学意义(P<0.05或P<0.01),SIRT1和Ki-67蛋白及mRNA表达水平降低,但Ki-67的mRNA表达差异无统计学意义(P>0.05)。与模型组比较,壮腰通络方各干预组病理损伤均有不同程度改善,其中中剂量组对椎间盘结构和基质的保护作用最佳。HIF-1α、p16蛋白及mRNA表达水平降低,差异有统计学意义(P<0.05或P<0.01); SIRT1蛋白及mRNA表达水平升高,差异有统计学意义(P<0.05或P<0.01); Ki-67蛋白及mRNA表达差异无统计学意义(P>0.05)。结论:壮腰通络方可以抑制大鼠椎间盘髓核细胞衰老,改善椎间盘组织损伤,从而发挥延缓腰椎间盘退变的作用,其机制可能与调节SIRT1/HIF-1α信号通路有关。
Abstract:
Objective:Based on the theory of “invigorating the kidney and activating blood circulation”,this study aims to investigate the mechanism by which Zhuangyao Tongluo formula(ZYTLF)delays intervertebral disc degeneration(IVDD)by inhibiting nucleus pulposus cell(NPC)senescence through regulation of the SIRT1/HIF-1α signaling pathway.Methods:Seventy 8-week-old SPF male rats were randomly divided into six groups:blank group,model group,positive drug control group(Celecoxib),and Zhuangyao Tongluo formula low-dose,medium-dose,and high-dose groups.Except for the blank group,a lumbar instability-induced IVDD model was established in all other groups by resection of the spinous processes,articular processes,and supraspinous ligaments.Intragastric administration was initiated on the third day after modeling and continued for 8 weeks.Behavioral performance was observed,and intervertebral disc and nucleus pulposus tissues were collected.Hematoxylin-eosin(HE)staining and Safranin O-Fast Green(SO/FG)staining were used to observe the morphology of the intervertebral disc tissues.Western Blot and real-time quantitative PCR(RT-qPCR)were employed to detect the protein and mRNA expression levels of SIRT1,HIF-1α,p16,and Ki-67 in the nucleus pulposus tissues.Results:Compared with the blank group,the nucleus pulposus tissues in the model group exhibited shrinkage and aggregation,disordered arrangement of the annulus fibrosus,and severe loss of matrix proteoglycans.The protein and mRNA expression levels of HIF-1α and p16 were significantly increased(P<0.05 or P<0.01),while the protein and mRNA expression levels of SIRT1 and Ki-67 were decreased; however,the difference in Ki-67 mRNA expression was not statistically significant(P>0.05).Compared with the model group,the pathological damage in all ZYTLF intervention groups was alleviated to varying degrees,with the medium-dose group showing the most significant protective effect on disc structure and matrix.The protein and mRNA expression levels of HIF-1α and p16 were significantly decreased(P<0.05 or P<0.01),and the protein and mRNA expression levels of SIRT1 were significantly increased(P<0.05 or P<0.01).There were no statistically significant differences in the protein and mRNA expression levels of Ki-67(P>0.05).Conclusion:Zhuangyao Tongluo formula can inhibit the senescence of rat nucleus pulposus cells and ameliorate intervertebral disc tissue injury,thereby playing a role in delaying IVDD.Its mechanism may be related to the regulation of the SIRT1/HIF-1α signaling pathway.

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(收稿日期:2025-12-03)

备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年科学基金项目(82205152)
中国中医科学院优秀青年科技人才(创新类)项目(ZZ16-YQ-023)
中华中医药学会青年人才托举工程项目(CACM-2023-QNRC2-A06)
△通信作者 E-mail:tcmsky@163.com
更新日期/Last Update: 2026-01-15