[1]陈燕超,林涛,郑梓灌,等.肝肾阴虚证和脾肾阳虚证老年性骨质疏松症患者肠道菌群分布特征[J].中国中医骨伤科杂志,2025,33(12):68-74+80.[doi:10.20085/j.cnki.issn1005-0205.251212]
　CHEN Yanchao,LIN Tao,ZHENG Ziguan,et al.Gut Microbiota Profiles in Senile Osteoporosis:Liver-Kidney Yin Deficiency vs Spleen-Kidney Yang Deficiency Syndromes[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(12):68-74+80.[doi:10.20085/j.cnki.issn1005-0205.251212]
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肝肾阴虚证和脾肾阳虚证老年性骨质疏松症患者肠道菌群分布特征()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年12期
页码:
68-74+80
栏目:
论著
出版日期:
2025-12-15

文章信息/Info

Title:
Gut Microbiota Profiles in Senile Osteoporosis:Liver-Kidney Yin Deficiency vs Spleen-Kidney Yang Deficiency Syndromes
文章编号:
1005-0205(2025)12-0068-07
作者:
陈燕超1; 林涛1; 郑梓灌1; 杨婧1; 罗观金1; 张建新2△; 何剑全3
1厦门医学院附属第二医院(福建 厦门,361021)
2北京中医药大学东直门医院厦门医院
3厦门大学附属中山医院
Author(s):
CHEN Yanchao1; LIN Tao1; ZHENG Ziguan1; YANG Jing1; LUO Guanjin1; ZHANG Jianxin2△; HE Jianquan3
1The Second Affiliated Hospital of Xiamen Medical College,Xiamen 361021,Fujian China; 2Beijing University of Chinese Medicine Xiamen Hospital,Xiamen 361015,Fujian China; 3Zhongshan Hospital,Xiamen University,Xiamen 361004,Fujian China.
关键词:
老年性骨质疏松症;  肠道菌群;  肝肾阴虚证;  脾肾阳虚证
Keywords:
senile osteoporosis;  gut microbiota;  liver-kidney Yin deficiency;  spleen-kidney Yang deficiency
分类号:
R681
DOI:
10.20085/j.cnki.issn1005-0205.251212
文献标志码:
A
摘要:
目的:探讨肝肾阴虚证和脾肾阳虚证老年性骨质疏松症(SOP)患者肠道菌群的特征及差异,为老年性骨质疏松症患者适合补充的肠道益生菌提供理论依据。方法:经中医辨证分型分为肝肾阴虚组和脾肾阳虚组,采集老年性骨质疏松症患者的临床资料及粪便标本各30例,双能X线骨密度仪检测骨密度,并检测血清总Ⅰ型胶原氨基端延长肽(TP1NP)及血清Ⅰ型胶原羧基端肽β特殊序列(β-CTX)水平,采用16S rDNA测序技术分析两组患者肠道菌群结构和丰度的差异。分析肠道菌群与骨密度和β-CTX、TP1NP的相关性,分析肠道菌群与老年性骨质疏松症的相关性。结果:α-多样性分析结果显示,两组不同中医证型患者的肠道菌群物种多样性、丰富度和均匀度差异均无统计学意义(P>0.05); β-多样性分析结果表明,两组不同中医证型患者的肠道菌群组间差异无统计学意义(P=0.342)。通过LEfSe分析,在属水平上,肝肾阴虚组中普雷沃氏菌属(Prevotella)在相对丰度上具有优势,而脾肾阳虚组则是科林塞拉拟杆菌(Collinsella)、鼠杆菌属(Muribaculaceae)、链球菌属(Streptococcus)的占比较大。两组患者菌群数据显示与年龄正相关的菌属有链球菌属; 与骨密度正相关菌属有Victivallaceae,与TP1NP正相关的菌属有Eggerthella,负相关的有Ralstonia。结论:肝肾阴虚证和脾肾阳虚证老年性骨质疏松症患者具有各自的肠道菌群特征,这些菌群与骨代谢标志物及骨密度具有相关性,老年性骨质疏松症患者可以考虑适量补充相应菌群。
Abstract:
Objective:To investigate the characteristics and differences in gut microbiota between senile osteoporosis(SOP)patients with liver-kidney Yin deficiency syndrome and those with spleen-kidney Yang deficiency syndrome.The findings will provide a theoretical basis for identifying appropriate probiotic supplements tailored to SOP patients.Methods:Patients were classified into liver-kidney Yin deficiency and spleen-kidney Yang deficiency groups based on traditional Chinese medicine(TCM)syndrome differentiation.Clinical data and fecal samples were collected from 30 SOP patients in each group.Bone mineral density(BMD)was measured using dual-energy X-ray absorptiometry(DXA).Serum levels of total type Ⅰ procollagen amino-terminal propeptide(TP1NP)and β-carboxy-terminal cross-linked telopeptide of type Ⅰ collagen(β-CTX)were analyzed.The gut microbiota composition and relative abundance in the two groups were compared via 16S rDNA sequencing.The correlations between gut flora and bone mineral density,β-CTX,and TP1NP were analyzed.And the correlations between gut flora and SOP were analyzed.Results:The α-diversity analysis showed no statistically significant differences in species diversity,richness,and evenness of gut flora between the two groups(P>0.05).The β-diversity analysis indicated that there were no significant differences in the composition of gut flora between the two groups(P=0.342).Through LEfSe analysis,at the genus level,Prevotella was more abundant in the “liver-kidney Yin deficiency” type group,while Collinsella,Muribaculaceae,and Streptococcus were more abundant in the “spleen-kidney Yang deficiency” type group.The data of the two groups of patients showed that Streptococcus was positively correlated with age; Victivallaceae was positively correlated with bone mineral density; Eggerthella was positively correlated with TP1NP,while Ralstonia was negatively correlated.Conclusion:Patients with SOP exhibiting liver-kidney Yin deficiency syndrome and spleen-kidney Yang deficiency syndrome demonstrate distinct characteristic gut microbiota profiles.These microbial communities are correlated with bone metabolism markers and bone mineral density.Accordingly,targeted supplementation with specific bacterial strains may represent a potential therapeutic consideration for SOP management.

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(收稿日期:2025-05-09)

备注/Memo

备注/Memo:
基金项目:厦门市科技局指导性项目(3502Z20224ZD1255)
△通信作者 E-mail:fjxmzjx@163.com
更新日期/Last Update: 2025-12-15