[1]蒋德斌△,熊俊宇,黄拯.杜鹃素对创伤性骨折大鼠骨折愈合的影响及作用机制探讨[J].中国中医骨伤科杂志,2025,33(11):9-13.[doi:10.20085/j.cnki.issn1005-0205.251102]
　JIANG Debin,XIONG Junyu,HUANG Zheng.A Study on the Effects and Mechanisms of Farrerol on Fracture Healing in Rats with Traumatic Bone Fractures[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(11):9-13.[doi:10.20085/j.cnki.issn1005-0205.251102]
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杜鹃素对创伤性骨折大鼠骨折愈合的影响及作用机制探讨()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年11期
页码:
9-13
栏目:
实验研究
出版日期:
2025-11-15

文章信息/Info

Title:
A Study on the Effects and Mechanisms of Farrerol on Fracture Healing in Rats with Traumatic Bone Fractures
文章编号:
1005-0205(2025)11-0009-05
作者:
蒋德斌1△; 熊俊宇1; 黄拯1
1成都八一骨科医院(成都,610091)
Author(s):
JIANG Debin1△; XIONG Junyu1; HUANG Zheng1
1Chengdu Bayi Orthopaedic Hospital,Chengdu 610091,China.
关键词:
杜鹃素;  干扰素基因刺激蛋白;  核因子κB;  创伤性骨折;  骨折愈合
Keywords:
farrerol;  stimulator of interferon gene;  nuclear factor κB;  traumatic fracture;  fracture healing
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.251102
文献标志码:
A
摘要:
目的:基于干扰素基因刺激蛋白/核因子-κB(STING/NF-κB)信号通路探究杜鹃素(Far)对创伤性骨折大鼠骨折愈合的影响及作用机制。方法:构建创伤性骨折大鼠模型,将建模成功的大鼠分为骨折组、杜鹃素低剂量组、杜鹃素高剂量组(12.5,50.0 mg/kg杜鹃素灌胃)、杜鹃素高剂量+DMXAA组(50 mg/kg杜鹃素灌胃+尾部静脉注射25 mg/kg 干扰素基因刺激蛋白激活剂5,6-二甲呫吨酮-4-乙酸(DMXAA)),每组各10只。另取10只正常大鼠为正常对照组,正常对照组与骨折组给予等量生理盐水,1次/d,连续4周。计算机断层扫描(CT)检测大鼠骨小梁密度和骨厚度; 番红-O染色观察大鼠软骨组织病理变化; 试剂盒检测大鼠血清生化指标; ELISA检测大鼠炎症因子水平; Western Blot法检测大鼠软骨组织中STING/NF-κB信号通路蛋白表达。结果:骨折组大鼠较正常对照组软骨组织损伤严重,软骨破裂、不完整; 杜鹃素低剂量组及杜鹃素高剂量组大鼠较骨折组软骨组织损伤程度降低; 杜鹃素高剂量+DMXAA组大鼠较杜鹃素高剂量组软骨组织损伤加重。骨折组大鼠较正常对照组骨小梁密度、骨厚度、骨钙素、碱性磷酸酶水平降低,TNF-α、IL-6水平及STING、p-NF-κB p65/NF-κB p65蛋白表达升高,差异有统计学意义(P<0.05); 杜鹃素低剂量组和杜鹃素高剂量组大鼠较骨折组骨小梁密度、骨厚度、骨钙素、碱性磷酸酶水平升高,TNF-α、IL-6水平及STING、p-NF-κB p65/NF-κB p65蛋白表达降低,差异有统计学意义(P<0.05); 杜鹃素高剂量+DMXAA组大鼠较杜鹃素高剂量组骨小梁密度、骨厚度、骨钙素、碱性磷酸酶水平降低,TNF-α、IL-6水平及STING、p-NF-κB p65/NF-κB p65蛋白表达升高,差异有统计学意义(P<0.05)。结论:杜鹃素可通过抑制STING/NF-κB信号通路促进创伤性骨折大鼠骨折愈合。
Abstract:
Objective:To investigate the effects of farrerol(Far)and the mechanisms involved in fracture healing in rats with traumatic fractures,based on the STING/NF-κB signaling pathway.Methods:A traumatic fracture rat model was constructed,and successfully modeled rats were classified into fracture group,Far low-dose group,Far high-dose group(gavage of 12.5,50.0 mg/kg Far),Far high-dose+DMXAA group(gavage of 50 mg/kg Far+tail vein injection of 25 mg/kg STING activator DMXAA),each had 10 rats.Another 10 normal rats were served as the normal contrast(NC)group.The NC group and fracture group were given equal amounts of physiological saline,once a day for 4 consecutive weeks.Computed tomography was used to measure trabecular density and bone thickness in rats.Safranin-O staining was used to observe pathological changes in rat cartilage tissue.The reagent kit was used to detect biochemical indicators in rat serum.ELISA was used to measure the inflammatory cytokines in rats.Western Blot was used to measure the STING/NF-κB signaling pathway proteins in rat cartilage tissue.Results:The cartilage tissue of rats in the fracture group was severely damaged than the NC group,with cartilage rupture and incompleteness.The degree of cartilage tissue damage in the Far low-dose group and Far high-dose group was lower than the fracture group.The cartilage tissue damage in the Far high-dose+DMXAA group was more severe than that in the Far high-dose group.The fracture group had decreased trabecular density,bone thickness,BGP,and ALP,and increased TNF-α,IL-6 levels,STING,and p-NF-κB p65/NF-κB p65 proteins than the NC group(P<0.05).The Far low-dose group and Far high-dose group had increased trabecular density,bone thickness,BGP,and ALP,and decreased TNF-α,IL-6 levels,STING,and p-NF-κB p65/NF-κB p65 proteins than the fracture group(P<0.05).The Far high-dose group+DMXAA group had decreased trabecular density,bone thickness,BGP,and ALP,and increased TNF-α,IL-6 levels,STING,and p-NF-κB p65/NF-κB p65 proteins than the Far high-dose group(P<0.05).Conclusion:Far can promote fracture healing in rats with traumatic fracture by inhibiting STING/NF-κB signaling pathway.

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(收稿日期:2025-04-15)

备注/Memo

备注/Memo:
基金项目:成都市医学科研课题(2022032)
△通信作者 E-mail:jgu810@126.com
更新日期/Last Update: 2025-11-15