[1]杨光亚,白玉,邓磊,等.活络骨康丸防治兔激素性股骨头坏死的实验研究[J].中国中医骨伤科杂志,2025,33(10):1-7.[doi:10.20085/j.cnki.issn1005-0205.251001]
　YANG Guangya,BAI Yu,DENG Lei,et al.Experimental Study on Huoluogukang Pill for Preventing and Treating Steroid-Induced Osteonecrosis of the Femoral Head in Rabbits[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(10):1-7.[doi:10.20085/j.cnki.issn1005-0205.251001]
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活络骨康丸防治兔激素性股骨头坏死的实验研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年10期
页码:
1-7
栏目:
实验研究
出版日期:
2025-10-15

文章信息/Info

Title:
Experimental Study on Huoluogukang Pill for Preventing and Treating Steroid-Induced Osteonecrosis of the Femoral Head in Rabbits
文章编号:
1005-0205(2025)10-0001-07
作者:
杨光亚1; 白玉2; 邓磊1; 栾帅宝1; 王少华2△
1河南中医药大学(郑州,450046)
2郑州市骨科医院
Author(s):
YANG Guangya1; BAI Yu2; DENG Lei1; LUAN Shuaibao1; WANG Shaohua2△
1Henan University of Chinese Medicine,Zhengzhou 450046,China; 2Zhengzhou Orthopedic Hospital,Zhengzhou 450052,China.
关键词:
激素性股骨头坏死;  过氧化物酶体增殖物激活受体γ;  促炎/抗炎因子;  活络骨康丸
Keywords:
steroid-induced osteonecrosis of the femoral head;  peroxisome proliferators-activated receptor gamma;  inflammatory/anti-inflammatory cytokines;  Huoluogukang pill
分类号:
R681.8
DOI:
10.20085/j.cnki.issn1005-0205.251001
文献标志码:
A
摘要:
目的:观察活络骨康丸对兔激素性股骨头坏死(SONFH)的防治作用,探究其作用机制。方法:选取48只普通级新西兰兔,随机分为6组(每组各8只):空白组、模型组、活络骨康丸低剂量组、活络骨康丸中剂量组、活络骨康丸高剂量组和阳性药物组。通过异体血清联合激素诱导股骨头坏死模型,预防性给予各组实验兔治疗药物6周,活络骨康丸低、中、高剂量组分别用低、中、高剂量活络骨康丸悬浊液灌胃治疗,阳性药物组用普伐他汀药液灌胃治疗。治疗结束后,MRI检测髋关节影像学改变,苏木精-伊红染色(HE)切片观察组织形态学病理变化,测定血清总胆固醇(CHO)、高密度脂蛋白胆固醇(HDL)和低密度脂蛋白胆固醇(LDL)水平,ELISA检测血清中细胞因子TNF-α、IL-1β、IL-6、IL-10和IL-4水平,RT-qPCR检测股骨头组织中过氧化物酶体增殖物激活受体γ(PPARγ)和诱导型一氧化氮合酶(iNOS)的mRNA表达水平,Western Blot检测股骨头组织中PPARγ、CCAAT/增强子结合蛋白α(C/EBPα)和iNOS蛋白表达水平。结果:MRI扫描显示模型组T2WI可见股骨头软骨下骨区域表现出低信号强度的带状线,空白组、活络骨康丸低、中、高剂量组和阳性药物组未见明显异常。病理切片显示活络骨康丸低、中、高剂量组和阳性药物组较之模型组坏死灶减少,空骨陷窝率低、骨小梁面积升高,差异有统计学意义(P<0.05)。与模型组相比,活络骨康丸中、高剂量组血清总胆固醇、低密度脂蛋白胆固醇下降,高密度脂蛋白胆固醇升高,差异有统计学意义(P<0.05); 血清TNF-α、IL-1β和IL-6显著降低,IL-10和IL-4升高,差异有统计学意义(P<0.05); PPARγ和iNOS mRNA的表达水平下降,差异有统计学意义(P<0.05); PPARγ、C/EBPα和iNOS蛋白表达水平降低,差异有统计学意义(P<0.001)。结论:活络骨康丸可通过抑制PPARγ通路,纠正脂代谢的失衡,维持骨稳态,同时能够抑制激素性股骨头坏死中免疫因子介导的慢性炎症,达到防治激素性股骨头坏死的治疗目的。
Abstract:
Objective:To investigate the therapeutic and preventive effects of Huoluogukang pill on steroid-induced osteonecrosis of the femoral head(SONFH)in rabbits and explore its mechanisms.Methods:48 New Zealand rabbits were randomly divided into 6 groups(n=8 per group):blank group,model group,low-dose Huoluogukang group,medium-dose Huoluogukang group,high-dose Huoluogukang group,and positive drug group.SONFH models were induced by heterologous serum combined with hormone injections.low-dose,medium-dose,and high-dose Huoluogukang suspensions were given via gavage to the respective groups,while the positive drug group received pravastatin sodium solution.Post-treatment evaluations included MRI for hip joint imaging,hematoxylin-eosin(HE)staining for histopathological analysis,automatic biochemical analyzer for serum total cholesterol(CHO),high-density lipoprotein cholesterol(HDL),and low-density lipoprotein cholesterol(LDL),ELISA for serum cytokines(TNF-α,IL-1β,IL-6,IL-10,IL-4),RT-qPCR for mRNA expression of PPARγ and iNOS in femoral head tissue,and Western Blot for PPARγ,C/EBPα,and iNOS protein expression.Results:MRI scans revealed that the model group exhibited low-signal-intensity banded lines in the subchondral bone region of the femoral head on T2WI,whereas no significant abnormalities were observed in the blank group,low-dose/medium-dose/high-dose Huoluogukang groups,or the positive drug group.Histopathological analysis demonstrated that:compared to the model group,the Huoluogukang-treated groups(low dose,medium dose,high dose)and the positive drug group showed reduced necrotic foci,a lower empty lacunae rate,and increased trabecular bone area,with statistically significant differences(P<0.05).Serum total cholesterol(CHO)and low-density lipoprotein cholesterol levels decreased,while high-density lipoprotein cholesterol increased significantly in the medium-dose and high-dose Huoluogukang groups compared to the model group(P<0.05).Serum pro-inflammatory cytokines(TNF-α,IL-1β,IL-6)were markedly reduced,and anti-inflammatory cytokines(IL-10,IL-4)were elevated in the Huoluogukang-treated groups(P<0.05).Additionally,mRNA expression levels of PPARγ and iNOS in femoral head tissue were downregulated(P<0.05),and protein expression levels of PPARγ,C/EBPα,and iNOS were significantly reduced(P<0.001).Conclusion:Huoluogukang pills can prevent and treat SONFH by inhibiting the PPARγ pathway,correcting lipid metabolism imbalance,maintaining bone homeostasis,and inhibiting chronic inflammation mediated by immune factors in SONFH.

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(收稿日期:2025-05-02)

备注/Memo

备注/Memo:
基金项目:河南省中医药科学研究专项课题(2024ZY1034)
郑州市科技惠民项目(2024KJHM0017)
△通信作者 E-mail:wangshaohua1719@163.com
更新日期/Last Update: 2025-10-10