[1]庞呈祥 姜红江 王亚楠 鞠昌军 余昕 侯燕 王一迪△.骨碎补联合羟基磷灰石-壳聚糖支架修复兔大段骨缺损的实验研究[J].中国中医骨伤科杂志,2025,33(09):1-8.[doi:10.20085/j.cnki.issn1005-0205.250901]
　PANG Chengxiang JIANG Hongjiang WANG Yanan JU Changjun YU Xin HOU Yan WANG Yidi.Experimental Study on the Repair of Large Segmental Bone Defects in Rabbits by Drynaria Rhizome Combined with Hydroxyapatite-Chitosan Scaffolds[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(09):1-8.[doi:10.20085/j.cnki.issn1005-0205.250901]
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骨碎补联合羟基磷灰石-壳聚糖支架修复兔大段骨缺损的实验研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年09期
页码:
1-8
栏目:
实验研究
出版日期:
2025-09-15

文章信息/Info

Title:
Experimental Study on the Repair of Large Segmental Bone Defects in Rabbits by Drynaria Rhizome Combined with Hydroxyapatite-Chitosan Scaffolds
文章编号:
1005-0205(2025)09-0001-08
作者:
庞呈祥1; 2 姜红江1; 2 王亚楠1; 2 鞠昌军1; 2 余昕1; 2 侯燕1; 2 王一迪1△
1山东省文登整骨医院(山东 威海,264400)
2山东省中医骨伤研究院
Author(s):
PANG Chengxiang1; 2 JIANG Hongjiang1; 2 WANG Yanan1; 2 JU Changjun1; 2 YU Xin1; 2 HOU Yan1; 2 WANG Yidi1△
1Shandong Wendeng Orthopedic Hospital,Weihai 264400,Shandong China; 2Shandong Institute of Orthopedics and Traumatology,Weihai 264400,Shandong China.
关键词:
骨碎补;  羟基磷灰石-壳聚糖;  大段骨缺损;  骨修复
Keywords:
Drynaria Rhizome;  hydroxyapatite-chitosan;  large segmental bone defects;  bone repair
分类号:
R318.08
DOI:
10.20085/j.cnki.issn1005-0205.250901
文献标志码:
A
摘要:
目的:探索中药骨碎补联合羟基磷灰石-壳聚糖支架修复兔大段骨缺损的可行性。方法:取24只新西兰大白兔,用电锯在双侧桡骨中段制造1.5 cm长的骨缺损区,选择左侧骨缺损旷置(作为空白对照),右侧植入羟基磷灰石-壳聚糖支架,无差别平分成两组。观察组(A组)给予骨碎补煎液(1 g/mL)灌胃(2次/d),每次灌胃剂量为6.1 g/kg(约为15 mL),对照组(B组)给予相应生理盐水灌胃(2次/d)。先后于术后第4周和第12周处死实验动物,分别记录A组、B组右侧(植入支架侧)为A1、B1,A组、B组左侧(空白对照侧)为A2、B2,进行大体标本观察、X线观察及组织学观察。结果:大体观察及X线片检查示术后第4周和第12周A1新生骨组织量明显多于A2,B1新生骨组织量明显多于B2,A1新生骨组织量多于B1,且术后第4周和第12周A1、B1的Lane-Sandhu-X线片评分显著高于A2、B2,A1 X线片评分高于B1,差异均有统计学意义(P<0.05)。组织学观察显示术后第4周A1截骨处见较多新生骨组织,以软骨成骨方式向骨小梁过渡,支架降解明显; B1截骨处见纤维性软骨,未见新生骨小梁,支架降解不明显; 术后第12周A1截骨处见骨改造塑形及大量骨单位形成(以骨细胞为主),B1截骨处见骨塑形较差,可见少量骨单位形成(以成骨细胞为主),A2截骨断端处仅见较少的钙盐沉积,B2截骨断端处未见新生骨组织及钙盐沉积,仅见少量胶原纤维。术后第4周和第12周A1、B1 Lane-Sandhu组织学评分显著高于A2、B2,且A1明显高于B1,差异均有统计学意义(P<0.05)。结论:羟基磷灰石-壳聚糖支架具有良好的生物相容性及骨传导能力,中药骨碎补(以灌胃方式给药)联合羟基磷灰石-壳聚糖支架能够促进骨缺损的新骨生成,缩短骨愈合时间,对兔大段骨缺损修复具有一定积极作用。
Abstract:
Objective:To explore the feasibility of repairing large segmental bone defects in rabbits by combining traditional Chinese medicine Drynaria Rhizome with hydroxyapatite-chitosan scaffolds.Methods:24 New Zealand large white rabbits were taken,and a 1.5 cm bone defect area was created in the middle radius bilaterally with a chainsaw.The left side of the bone defect was chosen to be left open(as blank control),and the right side was implanted with hydroxyapatite-chitosan scaffolds,and the observation group(group A)was given the decoction of Drynaria Rhizome(1 g/mL)by gastric gavage twice a day with the dosage of 6.1 g/kg(about 15 mL)per gavage,and the control group(group B)was given the corresponding saline by gastric gavage twice a day,successively after the operation).The control group(group B)was given the corresponding saline by gavage twice a day,and the experimental animals were executed at 4 and 12 weeks after the operation,and the right sides of group A and group B(implanted stent side)were recorded as A1 and B1,and the left sides of group A and group B(blank control side)were recorded as A2 and B2,respectively,and the macroscopic specimens,X-ray observation,and histology were carried out.Results:Gross observation and X-ray examination showed that the amount of new bone tissue of A1 was significantly more than that of A2,and the amount of new bone tissue of B1 was significantly more than that of B2 at 4 and 12 weeks after surgery,and the Lane-Sandhu-X-ray scores of A1 and B1 were significantly higher than those of A2 and B2 at 4 and 12 weeks after surgery,and the A1 X-ray score was higher than that of B1,and the differences were all statistically significant(P<0.05).Histological observation showed that more new bone tissue was seen at the A1 osteotomy at 4 weeks postoperatively,transitioning to trabeculae in a cartilaginous osteogenic manner,with obvious scaffold degradation; fibrous cartilage was seen at the B1 osteotomy,with no new trabeculae,and the scaffold degradation was not obvious.Bone remodeling was seen in A1 osteotomy at 12 weeks postoperatively,with a large number of bone units formed,dominated by osteoblasts,while poorer bone remodeling was seen in B1 osteotomy with a small amount of bone unit formation,dominated by osteoclasts,with A2 osteotomy dominated by osteoblasts.Osteoblasts were predominant,only a small amount of calcium salt deposition was seen at the A2 osteotomy,and no new bone tissue or calcium salt deposition was seen at the B2 osteotomy,only a small amount of collagen fibers were seen.The histologic scores of A1 and B1 Lane-Sandhu were significantly higher than those of A2 and B2 at 4 and 12 weeks after surgery,and A1 was significantly higher than B1,and the differences were statistically significant(P<0.05).Conclusion:Hydroxyapatite-chitosan scaffold has good biocompatibility and osteoconductivity,and the combination of traditional Chinese medicine Drynaria Rhizome with hydroxyapatite-chitosan scaffold in the form of gavage administration can promote the generation of new bone in the bone defect and shorten the time of bone healing,which has a certain positive effect on the repair of large segmental bone defects in rabbits.

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(收稿日期:2025-04-02)

备注/Memo

备注/Memo:
基金项目:威海市中医药科技项目(2022N-36)
△通信作者 E-mail:1370368678@qq.com
更新日期/Last Update: 2025-09-15