[1]张勇,周芸,刘晓珂,等.积雪草苷脂质体凝胶改善糖尿病足溃疡大鼠氧化应激的机制研究[J].中国中医骨伤科杂志,2025,33(08):7-11.[doi:10.20085/j.cnki.issn1005-0205.250802]
　ZHANG Yong,ZHOU Yun,LIU Xiaoke,et al.Mechanism Study of Asiaticoside Liposome Gel in Improving Oxidative Stress in Diabetic Foot Ulcer Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(08):7-11.[doi:10.20085/j.cnki.issn1005-0205.250802]
点击复制

积雪草苷脂质体凝胶改善糖尿病足溃疡大鼠氧化应激的机制研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年08期
页码:
7-11
栏目:
实验研究
出版日期:
2025-08-15

文章信息/Info

Title:
Mechanism Study of Asiaticoside Liposome Gel in Improving Oxidative Stress in Diabetic Foot Ulcer Rats
文章编号:
1005-0205(2025)08-0007-05
作者:
张勇1; 周芸1; 刘晓珂1; 喻伟光1△
1石家庄市人民医院(石家庄,050011)
Author(s):
ZHANG Yong1; ZHOU Yun1; LIU Xiaoke1; YU Weiguang1△
1Shijiazhuang People's Hospital,Shijiazhuang 050011,China.
关键词:
积雪草苷脂质体凝胶;  糖尿病足溃疡大鼠;  新生血管生成;  氧化应激
Keywords:
asiaticoside liposome gel;  diabetic foot ulcer rats;  neovascularization;  oxidative stress
分类号:
R587.2
DOI:
10.20085/j.cnki.issn1005-0205.250802
文献标志码:
A
摘要:
目的:探究积雪草苷脂质体凝胶通过Toll样受体4(TLR4)/核因子κB(NF-κB)通路对糖尿病足溃疡(DFU)大鼠氧化应激的影响。方法:将大鼠随机分为对照组、模型组(复制糖尿病后钕铁硼磁铁按压足背)、药物低剂量组、药物高剂量组及药物高剂量+激活剂组,每组12只。末次给药24 h后,检测创面愈合率; 酶联免疫吸附测定(ELISA)检测血清血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α),以及创面组织碱性成纤维细胞生长因子(bFGF)和血管性血友病因子(vWF)水平; 用试剂盒检测创面组织中超氧化物歧化酶(SOD)及丙二醛(MDA)水平; 苏木精-伊红(HE)染色观察创面组织病理变化; Western Blot法检测创面组织中TLR4/NF-κB通路相关蛋白表达。结果:相较于对照组,模型组创面愈合率、VEGF、bFGF、vWF、SOD水平降低,TNF-α、MDA、TLR4、p-NF-κB/NF-κB水平增高(P<0.05); 相较于模型组,药物低剂量组和药物高剂量组创面愈合率、VEGF、bFGF、vWF、SOD增高,TNF-α、MDA、TLR4、p-NF-κB/NF-κB水平下降(P<0.05); TLR4激活剂可减弱药物高剂量对糖尿病足溃疡大鼠上述指标的影响(P<0.05)。结论:积雪草苷脂质体凝胶可能通过抑制TLR4/NF-κB信号通路,改善糖尿病足溃疡大鼠新生血管生成及氧化应激情况。
Abstract:
Objective:To investigate the impacts of asiaticoside liposome gel on neovascularization and oxidative stress in diabetic foot ulcer(DFU)rats through toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)pathways.Methods:Rats were stochastically assigned into control group,model group(after replicating diabetes,NdFeB magnet pressing on the dorsum of the foot),drug low-dose group,drug high-dose group,and drug high-dose + activator group,with 12 rats in each group.24 h after the last administration,the wound healing rate was measured.Enzyme-linked immunosorbent assay(ELISA)was used to detect vascular endothelial growth factor(VEGF),tumor necrosis faction-α(TNF-α)in the serum,and basic fibroblast growth factor(bFGF)and von Willefibrilia factor(vWF)in wound tissue.The reagent kit was used to detect superoxide dismutase(SOD)and malondialdehyde(MDA)in the wound tissues.The pathological changes of wound tissue was observed by hematoxylin-eosin(HE)staining; Western Blot was used to detect TLR4/NF-κB pathway related protein expression in wound tissue.Results:Compared with the control group,the wound healing rate,VEGF,bFGF,vWF,and SOD levels in model group were lower,while TNF-α,MDA,TLR4,and p-NF-κB/NF-κB were higher(P<0.05).Compared with the model group,the wound healing rate,VEGF,bFGF,vWF,and SOD in drug low-dose group and drug high-dose group were higher,while TNF-α,MDA,TLR4,and p-NF-κB/NF-κB were lower(P<0.05).TLR4 activator can reduce the effects of drug high-dose on DFU rats(P<0.05).Conclusion:Asiaticoside liposome gel may improve neovascularization and oxidative stress in DFU rats by inhibiting TLR4/NF-κB signaling pathway.

参考文献/References:

[1] CHEN L,SUN S,GAO Y,et al.Global mortality of diabetic foot ulcer:a systematic review and meta-analysis of observational studies[J].Diabetes Obes Metab,2023,25(1):36-45.
[2] 刘敏敏,李锂,马小来.积雪草苷柔性纳米脂质体凝胶剂的研制及体外评价[J].中国医药工业杂志,2013,44(11):1120-1122.
[3] NIE X,ZHANG H,SHI X,et al.Asiaticoside nitric oxide gel accelerates diabetic cutaneous ulcers healing by activating Wnt/β-catenin signaling pathway[J].Int Immunopharmacol,2020,79:106109.
[4] FRANZ S,ERTEL A,ENGEL K M,et al.Overexpression of S100A9 in obesity impairs macrophage differentiation via TLR4-NF-κB-signaling worsening inflammation and wound healing[J].Theranostics,2022,12(4):1659-1682.
[5] LIU K,YIN Y,SHI C,et al.Asiaticoside ameliorates DSS-induced colitis in mice by inhibiting inflammatory response,protecting intestinal barrier and regulating intestinal microecology[J].Phytother Res,2024,38(4):2023-2040.
[6] 雷霆,孙东旭,周军,等.基于Wnt/β-catenin信号通路的三七/白及胶海绵促进糖尿病足溃疡模型大鼠创面愈合的作用机制研究[J].中国药房,2019,30(4):483-487.
[7] 张晶,贾翠楠,何冰,等.金丝桃苷抑制Toll样受体4/髓样分化因子88/核因子-κB 信号通路减轻牙周炎大鼠牙周组织损伤实验研究[J].陕西医学杂志,2023,52(11):1463-1467.
[8] FENG L,LIU Y,CHEN Y,et al.Injectable antibacterial hydrogel with asiaticoside-loaded liposomes and ultrafine silver nanosilver particles promotes healing of burn-infected wounds[J].Adv Healthc Mater,2023,12(22):e2203201.
[9] LEE J H,KIM H L,LEE M H,et al.Asiaticoside enhances normal human skin cell migration,attachment and growth in vitro wound healing model[J].Phytomedicine,2012,19(13):1223-1227.
[10] SGUIZZATO M,ESPOSITO E,CORTESI R.Lipid-based nanosystems as a tool to overcome skin barrier[J].Int J Mol Sci,2021,22(15):8319.
[11] 黎珊珊,区岛良,张梅,等.大黄酚通过核因子E2相关因子2/血红素氧合酶-1信号通路对糖尿病足溃疡大鼠新生血管生成及氧化应激的影响[J].陕西医学杂志,2023,52(12):1660-1664.
[12] 刘吉斌,周宇,于庆瑞.正骨紫金丸对骨关节炎大鼠软骨组织的保护作用及其机制[J].中国中医骨伤科杂志,2023,31(8):7-12.
[13] TANG J,XU L,ZENG Y,et al.Effect of gut microbiota on LPS-induced acute lung injury by regulating the TLR4/NF-κB signaling pathway[J].Int Immunopharmacol,2021,91:107272.
[14] 徐琛,徐辉甫,苏文.积雪草苷对哮喘幼鼠肺组织炎症和Toll样受体4/核因子-κB信号影响[J].中国临床药理学杂志,2022,38(6):546-550.
[15] WANG N,HONG B,ZHAO Y,et al.Dopamine-grafted oxidized hyaluronic acid/gelatin/cordycepin nanofiber membranes modulate the TLR4/NF-κB signaling pathway to promote diabetic wound healing[J].Int J Biol Macromol,2024,262(Pt 1):130079.

备注/Memo

备注/Memo:
基金项目:石家庄市科技计划项目(231200903)
△通信作者 E-mail:xuar7n@163.com
更新日期/Last Update: 2025-08-15