[1]韦志坤 杨芳 邵菲 杨金杰 王旭东 李树彬 刘志翔 刘杰 任少海.淫羊藿苷促骨髓间充质干细胞骨向分化的实验研究[J].中国中医骨伤科杂志,2025,33(07):24-29.[doi:10.20085/j.cnki.issn1005-0205.250705 ]
　WEI Zhikun YANG Fang SHAO Fei YANG Jinjie WANG Xudong LI Shubin LIU Zhixiang LIU Jie REN Shaohai.The Effect of Icariin in Promoting Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(07):24-29.[doi:10.20085/j.cnki.issn1005-0205.250705 ]
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淫羊藿苷促骨髓间充质干细胞骨向分化的实验研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年07期
页码:
24-29
栏目:
实验研究
出版日期:
2025-07-15

文章信息/Info

Title:
The Effect of Icariin in Promoting Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
文章编号:
1005-0205(2025)07-0024-06
作者:
韦志坤1 杨芳2 邵菲1 杨金杰1 王旭东1 李树彬1 刘志翔1 刘杰1 任少海1
1邯郸市第一医院(河北 邯郸,056000)
2邯郸市中心医院
Author(s):
WEI Zhikun1 YANG Fang2 SHAO Fei1 YANG Jinjie1 WANG Xudong1 LI Shubin1 LIU Zhixiang1 LIU Jie1 REN Shaohai1
1Handan First Hospital,Handan 056000,Hebei China; 2Handan Central Hospital,Handan 056000,Hebei China.
关键词:
淫羊藿苷;  骨髓间充质干细胞;  骨向分化;  单磷酸腺苷活化蛋白激酶;  沉默信息调节因子2同系物1;  氧化物酶体增殖物激活受体γ共激活剂-1α
Keywords:
icariin;  bone marrow mesenchymal stem cells;  osteogenic differentiation;  adenosine monophosphate activated protein kinase;  silent information regulatory factor 2 homolog 1;  peroxisome proliferator activated receptor-γ co-activator-1α
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250705
文献标志码:
A
摘要:
目的:探讨淫羊藿苷在促骨髓间充质干细胞骨向分化过程中对单磷酸腺苷活化蛋白激酶(AMPK)/沉默信息调节因子2同系物1(SIRT1)/氧化物酶体增殖物激活受体γ共激活剂-1α(PGC1α)通路的影响。方法:以第3代骨髓间充质干细胞细胞为研究对象,将其分为对照组(正常培养)、骨向诱导组(在2 mmol/L L-谷氨酰胺、100 nmol/L地塞米松、10 mmol/L β-甘油磷酸酯、50 μmol/L L-抗坏血酸-2-磷酸脂中进行骨向诱导)、淫羊藿苷低、中、高剂量组(在骨向诱导的基础上添加0.01,0.10,1.00 μmol/L的淫羊藿苷)、抑制剂组(在淫羊藿苷高剂量组的基础上添加10 μmol/L AMPK抑制剂Compound C)。CCK-8法检测细胞增殖; 茜素红染色检测细胞矿化能力; ELISA试剂盒检测细胞中碱性磷酸酶(ALP)的表达; Western Blot法检测细胞中骨形态发生蛋白-2(BMP-2)、骨保护素(OPN)和AMPK/SIRT1/PGC1α通路蛋白表达。结果:对照组无矿化结节形成; 骨向诱导组有明显的矿化结节; 淫羊藿苷低、中、高剂量组矿化结节直径变大,染色变深,数量增多; 抑制剂组矿化结节颜色变浅,结节变少。骨向诱导组OD450值、ALP、BMP-2、OPG、p-AMPK/AMPK、SIRT1、PGC1α表达高于对照组(P<0.05); 淫羊藿苷低、中、高剂量组OD450值、ALP、BMP-2、OPG、p-AMPK/AMPK、SIRT1、PGC1α表达高于骨向诱导组(P<0.05); 抑制剂组OD450值、ALP、BMP-2、OPG、p-AMPK/AMPK、SIRT1、PGC1α表达低于淫羊藿苷高剂量组(P<0.05)。结论:淫羊藿苷可以促进骨髓间充质干细胞的骨向分化,其机制可能是通过激活AMPK/SIRT1/PGC1α通路实现的。
Abstract:
Objective:To investigate the effect of icariin on the adenosine monophosphate activated protein kinase(AMPK)/silent information regulatory factor 2 homolog 1(SIRT1)/peroxisome proliferator activated receptor-γ co-activator-1α(PGC1α)pathway in promoting osteogenic differentiation of bone marrow mesenchymal stem cells.Methods:The third generation bone marrow mesenchymal stem cells were assigned into control group(normal culture),osteogenic induction group(osteogenic induction in 2 mmol/L L-glutamine,100 nmol/L dexamethasone,10 mmol/L β-glycerophosphate,50 μmol/L L-ascorbate-2-phosphate),icariin low,medium and high doses groups(adding 0.01,0.10,1.00 μmol/L icariin on the basis of osteogenic induction),and inhibitor group(adding 10 μmol/L AMPK inhibitor compound C on the basis of icariin high dose group).CCK-8 method was applied to detect the proliferation of cell.Alizarin red staining was applied to detect the mineralization ability of cell.ELISA kit was applied to detect the expression of alkaline phosphatase(ALP)in cell.Western Blot was applied to detect the expression of bone morphogenice protein-2(BMP-2),osteoprotegerin(OPN),and AMPK/SIRT1/PGC1α pathway proteins in cell.Results:No mineralized nodules were formed in the control group; the osteogenic induction group had obvious mineralized nodules; the diameter,staining depth,and quantity of mineralized nodules increased in the icariin low,medium and high doses group,while the color of mineralized nodules reduced and the number of nodules decreased in the inhibitor group.The OD450 value,ALP,BMP-2,OPG,p-AMPK/AMPK,SIRT1,and PGC1α expression in the osteogenic induction group were higher than those in the control group(P<0.05).The OD450 value,ALP,BMP-2,OPG,p-AMPK/AMPK,SIRT1,and PGC1α expression in the icariin low,medium and high doses group were higher than those in the osteogenic induction group(P<0.05).The OD450 value,ALP,BMP-2,OPG,p-AMPK/AMPK,SIRT1,and PGC1α expression in the inhibitor group were lower than those in the high doses group(P<0.05).Conclusion:Icariin can promote osteogenic differentiation of bone marrow mesenchymal stem cells,which may be achieved by activating the AMPK/SIRT1/PGC1α pathway.

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备注/Memo

备注/Memo:
基金项目:邯郸市科学技术研究与发展计划项目(1723208066-11)
更新日期/Last Update: 2025-07-15