[1]邓雄伟 古鹏 何智南 李强 袁海涛 吴杭 胡和军△.高良姜素对跟腱炎大鼠炎性损伤的抑制作用及其机制研究[J].中国中医骨伤科杂志,2025,33(07):1-6.[doi:10.20085/j.cnki.issn1005-0205.250701 ]
 DENG Xiongwei GU Peng HE Zhinan LI Qiang YUAN Haitao WU Hang HU Hejun.Effects of Galangin on Inhibiting IRAK-1/MAPK/NOS Signal Pathway in Rats with Achilles Tendon Infection[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(07):1-6.[doi:10.20085/j.cnki.issn1005-0205.250701 ]
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高良姜素对跟腱炎大鼠炎性损伤的抑制作用及其机制研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年07期
页码:
1-6
栏目:
实验研究
出版日期:
2025-07-15

文章信息/Info

Title:
Effects of Galangin on Inhibiting IRAK-1/MAPK/NOS Signal Pathway in Rats with Achilles Tendon Infection
文章编号:
1005-0205(2025)07-0001-06
作者:
邓雄伟1 古鹏1 何智南2 李强1 袁海涛1 吴杭1 胡和军1△
1江西中医药大学附属洪都中医院(南昌,330006)
2江西中医药大学研究生院
Author(s):
DENG Xiongwei1 GU Peng1 HE Zhinan2 LI Qiang1 YUAN Haitao1 WU Hang1 HU Hejun1△
1Nanchang Hongdu Hospital of Traditional Chinese Medicine Affiliated to Jiangxi University of Chinese Medicine,Nanchang 330006,China; 2Graduate School of Jiangxi University of Chinese Medicine,Nanchang 330004,China.
关键词:
跟腱炎 高良姜素 白细胞介素-1受体相关激酶 丝裂原活化蛋白激酶 一氧化氮合酶
Keywords:
achilles tendinitis galangin interleukin-1 receptor associated kinase mitogen-activated protein kinase nitric oxide synthase
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250701
文献标志码:
A
摘要:
目的:探讨高良姜素通过调控IRAK-1/MAPK/NOS信号通路对跟腱炎(AT)大鼠炎性损伤的影响及作用机制。方法:54只大鼠随机分为对照组、模型组、高良姜素组,对照组行假手术造模处理,其余大鼠通过Ⅰ型细菌胶原酶注射构建跟腱炎大鼠模型,造模后采用苏木精-伊红(HE)染色对跟腱炎大鼠进行验证。高良姜素组大鼠于左下肢跟腱中心点进行局部注射1次/d,连续2周,对照组和模型组同部位注射等量生理盐水。干预后行HE染色及Masson染色观察跟腱组织病理变化、炎症病灶及纤维化情况; 蛋白质免疫印迹法(Western Blot法)检测跟腱组织中诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)、IRAK-1、p38 MAPK蛋白表达情况。酶联免疫吸附测定法(ELISA法)检测血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)炎症因子水平。结果:干预2周后,HE染色和Masson染色结果显示模型组跟腱组织结构遭到严重破坏,可见组织大面积坏死、炎症病灶、纤维化及胶原沉积物显著增多,高良姜素组上述现象明显减轻。Western Blot法结果显示模型组大鼠跟腱组织p-eNOS(内皮型一氧化氮合酶在Ser1177位点发生磷酸化)、iNOS、IRAK-1、p38 MAPK的蛋白表达较对照组显著升高; 高良姜素组大鼠跟腱组织p-eNOS、iNOS、IRAK-1、p38 MAPK的表达较模型组显著下降。ELISA法结果显示模型组血清IL-1β、IL-6的水平较对照组显著升高; 高良姜素组大鼠血清IL-1β、IL-6的水平较模型组显著降低。结论:高良姜素可能通过抑制IRAK-1/MAPK/NOS信号通路,降低血清IL-1β、IL-6炎症因子水平,从而减轻跟腱炎大鼠的炎性损伤。
Abstract:
Objective:To explore the protective effect of galangin on the inflammatory injury of achilles tendinitis(AT)in rats and explore the mechanism.Methods:54 rats were randomly divided into control group,model group and galangin group,the control group was treated with sham operation,the rest rats were injected with type Ⅰ bacterial collagenase to establish AT rat model,and Hematoxylin-Eosin(HE)staining was used to verify the AT rat model after model establishment.The pathological changes,inflammatory foci and fibrosis of achilles tendon were observed by HE staining and Masson staining,the protein expressions of inducible nitric oxide synthase(iNOS),endothelial nitric oxide synthase(eNOS),IRAK-1 and p38 MAPK in achilles tendon were detected by Western Blot,and the levels of serum interleukin-1β(IL-1β)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).Results:After 2 weeks of intervention,HE staining and Masson staining showed that the structure of achilles tendon in model group was severely damaged,and large area of necrosis,inflammatory focus,fibrosis and collagen deposition were significantly increased,which were significantly reduced in galangin group.Compared with the control group,the expression of p-eNOS,iNOS,IRAK-1 and p38 MAPK in the rat achilles tendon tissue of galangin group were significantly decreased,and the levels of IL-1β,IL-6 in the rat achilles tendon tissue of galangin group were significantly increased.The levels of IL-1β and IL-6 in serum of rats in galangin group were significantly lower than those in model group.Conclusion:Galangin may reduce the levels of IL-1β and IL-6 in serum by inhibiting the IRAK-1/MAPK/NOS signal pathway,thus alleviating the inflammatory injury of AT rats.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江西省中医药管理局科技计划江西省自然科学基金资助项目
南昌市市级重点专科建设项目(洪卫体改字(2023)10号)
通信作者 E-mail:1069145757@qq.com
更新日期/Last Update: 2025-07-15