[1]朱志恒,丁佳滢,曹昱,等.磐龙玉热疗对风寒湿痹型膝骨关节炎抗炎镇痛作用的实验研究[J].中国中医骨伤科杂志,2025,33(05):1-5+12.[doi:10.20085/j.cnki.issn1005-0205.250501]
　ZHU Zhiheng,DING Jiaying,CAO Yu,et al.Experimental Study on the Anti-Inflammatory and Analgesic Effects of Panlong Jade Thermotherapy on Knee Osteoarthritis with Anemofrigid-Damp Arthralgia[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(05):1-5+12.[doi:10.20085/j.cnki.issn1005-0205.250501]
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磐龙玉热疗对风寒湿痹型膝骨关节炎抗炎镇痛作用的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年05期
页码:
1-5+12
栏目:
实验研究
出版日期:
2025-05-05

文章信息/Info

Title:
Experimental Study on the Anti-Inflammatory and Analgesic Effects of Panlong Jade Thermotherapy on Knee Osteoarthritis with Anemofrigid-Damp Arthralgia
文章编号:
1005-0205(2025)05-0001-05
作者:
朱志恒1; 2; 丁佳滢1; 2; 曹昱3; 葛杨硕1; 2; 黄春萌1; 2; 王学宗4; 沈军5; 谢金乐1; 2; 万潇霄1; 2; 邵与桐1; 2; 向延卫1; 2; 6; 丁道芳1; 2; 6△
1上海中医药大学康复医学院(上海,201203); 2上海市中医药研究院康复医学研究所; 3上海健康医学院附属周浦医院; 4上海中医药大学附属曙光医院石氏伤科医学中心; 5上海市光华中西医结合医院; 6上海中医药大学磐龙玉矿物药国际研究院
Author(s):
ZHU Zhiheng1; 2; DING Jiaying1; 2; CAO Yu3; GE Yangshuo1; 2; HUANG Chunmeng1; 2; WANG Xuezong4; SHEN Jun5; XIE Jinle1; 2; WAN Xiaoxiao1; 2; SHAO Yutong1; 2; XIANG Yanwei1; 2; 6; DING Daofang1; 2; 6△
1.School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Institute of Rehabilitation Science,Shanghai Academy of Traditional Chinese Medicine,Shanghai 201203,China;3.Shanghai University of Medicine &Health Sciences Affiliated Zhoupu Hospital,Shanghai 201318,China;4.Shi’s Center of Orthopedics and Traumatology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;
关键词:
磐龙玉;  风寒湿痹;  骨关节炎;  热疗;  镇痛;  中医疗法
Keywords:
Panlong Jade;  anemofrigid-damp arthralgia;  osteoarthritis;  thermotherapy;  analgesic effect;  TCM therapy
分类号:
R684.3
DOI:
10.20085/j.cnki.issn1005-0205.250501
文献标志码:
A
摘要:
目的:研究吉林磐龙玉热疗对风寒湿痹型膝骨关节炎(KOA)的抗炎镇痛作用,为中医矿物药在风寒湿痹症中的应用提供理论支持。方法:选择SPF级6周龄雄性SD大鼠20只,随机分为空白组、模型组、磁石组和磐龙玉组(各5只)。除空白组外,其余各组均采用改良Hulth法建立膝骨关节炎模型且术后第2周开始放入人工气候箱进行风寒湿干预。磁石组和磐龙玉组分别使用加热后的磁石和磐龙玉进行每日30 min的热疗,持续4周。干预结束后,用Von Frey纤维丝测定爪缩回疼痛阈值; 利用DigiGait成像系统测量步态参数; 用激光散斑成像系统检测膝关节血流灌注量; 用聚合酶链式反应(PCR)检测背根神经节中降钙素基因相关肽(CGRP)、瞬时受体电位香草酸亚家族成员1(TRPV1)、神经肽物质P(Substance P)、P75神经生长因子(P75)、神经肽Y(NPY)、转化生长因子-β(TGF-β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)等疼痛及炎症相关基因的表达; 用病理染色法观察关节破坏情况。结果:与模型组相比,在磁石及磐龙玉热疗干预后症状得到改善,且磐龙玉干预后改善效果更显著,差异有统计学意义(P<0.05); 磁石组及磐龙玉组足爪面积、制动时间及步幅明显增加,摆动时间明显缩短,其中磐龙玉组改善更明显,差异有统计学意义(P<0.05); 与空白组相比,模型组膝关节周围血流灌注明显减少,而磐龙玉热疗显著恢复了膝关节周围血流灌注,差异有统计学意义(P<0.05); 磐龙玉干预下调了大鼠背根神经节(DRG)组织中CGRP、TRPV1、Substance P、P75、IL-6和TNF-α等炎症及疼痛基因的相对表达水平,差异有统计学意义(P<0.05),但对NPY和TGF-β的表达水平无明显影响。结论:磐龙玉热疗具有活血化瘀、抗炎镇痛作用。
Abstract:
Objective:To investigate the effect of Panlong Jade thermotherapy on promoting blood circulation,dissipating blood stasis,and its anti-inflammatory and analgesic effects in the treatment of knee osteoarthritis with anemofrigid-damp arthralgia,providing theoretical support for the application of mineral-based traditional Chinese medicine.Methods:Twenty 6-week-old male SPF-grade Sprague-Dawley rats were randomly divided into blank group,model group,magnet group,and Panlong Jade group(5 rats each).Except for the blank group,a rat model of osteoarthritis was established by improved Hulth method in other groups.From the second postoperative week,the model group,magnet group,and Panlong Jade group were exposed to wind-cold-damp conditions in an artificial climate chamber.The magnet group and Panlong Jade group respectively received thermotherapy using heated magnets and heated Panlong Jade for 30 min a day for 4 weeks.After intervention,paw withdrawal thresholds were measured with Von Frey filaments,gait parameters were analyzed using DigiGait imaging system,knee joint blood perfusion was assessed by laser speckle imaging,expression of pain-related and inflammation-related genes(CGRP,TRPV1,Substance P,P75,NPY,TGF-β,IL-6,TNF-α)in the dorsal root ganglia(DRG)was detected by polymerase chain reaction(PCR).Results:Compared with the model group,Panlong Jade thermotherapy significantly improved the pain threshold in the affected limb,with a more pronounced effect than in the magnet group(P<0.05).Both the magnet and Panlong Jade groups showed increased paw area,stance time,and stride length,with reduced swing time,with Panlong Jade having a more substantial effect(P<0.05).Compared with the blank group,blood flow perfusion around the knee joint was significantly reduced in the model group,while Panlong Jade thermotherapy notably restored knee blood perfusion(P<0.05).Panlong Jade intervention significantly downregulated the relative expression levels of inflammatory and pain-related genes(CGRP,TRPV1,Substance P,P75,IL-6,and TNF-α)in DRG tissues(P<0.05),without significant effect on NPY and TGF-β expression.Conclusion:Panlong Jade thermotherapy has anti-inflammatory and analgesic effects.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81902306,82174406)
上海中医药大学科技发展项目(23KFL023)
上海健康医学院附属周浦医院院级人才项目(ZPRC-2024A-05)
△通信作者 E-mail:dingdaofang@shutcm.edu.cn
更新日期/Last Update: 2025-05-15