[1]师艳艳,康少英,杨武燕,等.杜仲总黄酮对腰椎间盘突出症大鼠的抗炎镇痛作用研究[J].中国中医骨伤科杂志,2025,33(04):11-15.[doi:10.20085/j.cnki.issn1005-0205.250403 ]
　SHI Yanyan,KANG Shaoying,YANG Wuyan,et al.Study on the Anti-Inflammatory and Analgesic Effects of Total Flavonoids from Eucommia Ulmoides on Rats with Lumbar Disc Herniation[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(04):11-15.[doi:10.20085/j.cnki.issn1005-0205.250403 ]
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杜仲总黄酮对腰椎间盘突出症大鼠的抗炎镇痛作用研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年04期
页码:
11-15
栏目:
实验研究
出版日期:
2025-04-10

文章信息/Info

Title:
Study on the Anti-Inflammatory and Analgesic Effects of Total Flavonoids from Eucommia Ulmoides on Rats with Lumbar Disc Herniation
文章编号:
1005-0205(2025)04-0011-05
作者:
师艳艳1; 康少英1; 杨武燕1; 张金盼1; 张凯1△
1邯郸市中心医院(河北 邯郸,056000)
Author(s):
SHI Yanyan1; KANG Shaoying1; YANG Wuyan1; ZHANG Jinpan1; ZHANG Kai1△
1Handan Central Hospital,Handan 056000,Hebei China.
关键词:
杜仲总黄酮;  CXC趋化因子配体12(CXCL12)和CXC趋化因子受体4(CXCR4)信号通路;  腰椎间盘突出症;  抗炎镇痛
Keywords:
total flavonoids of Eucommia ulmoides;  CXCL12/CXCR4 signal pathway;  lumbar disc herniation;  anti-inflammatory and analgesic
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250403
文献标志码:
A
摘要:
目的:探讨杜仲总黄酮调节CXCL12/CXCR4信号通路对腰椎间盘突出症(LDH)大鼠抗炎镇痛的影响。方法:随机选取10只大鼠作为假手术组,另取40只大鼠将髓核放在左侧L5和L6神经根顶部,构建腰椎间盘突出症大鼠模型,并分为模型组、药物组(200 mg/kg 杜仲总黄酮)、激活剂组(5 μg/kg 重组CXCL-12蛋白)及药物+激活剂组(200 mg/kg 杜仲总黄酮+5 μg/kg 重组CXCL-12蛋白),每组均为10只大鼠,假手术组和模型组给予等量生理盐水,连续治疗21 d。检测各组大鼠机械刺激及热刺激敏感性; 用ELISA试剂盒检测TNF-α、IL-1β水平; 通过HE染色观察脊髓背角组织病理变化; 用免疫荧光染色检测脊髓背角小胶质细胞活化情况; 用Western Blot法检测CXCL12/CXCR4信号通路相关蛋白水平。结果:杜仲总黄酮能增高腰椎间盘突出症大鼠的机械缩足反应阈值(PWT)值和热缩足反射潜伏期(TWL)值,降低IL-1β、TNF-α水平、Iba-1阳性小胶质细胞数量、CXCL12、CXCR4、NLRP3蛋白水平。杜仲总黄酮治疗后大鼠神经束排列较为整齐,炎性细胞浸润现象减轻; 而CXCL-12治疗后与杜仲总黄酮治疗后结果趋势相反,且消除了杜仲总黄酮对腰椎间盘突出症大鼠的抗炎镇痛作用。结论:杜仲总黄酮可能通过抑制CXCL12/CXCR4信号通路对腰椎间盘突出症大鼠发挥抗炎镇痛效果。
Abstract:
Objective:To investigate the impacts of total flavonoids of Eucommia ulmoides on inflammation and pain in rats with lumbar disc herniation(LDH)by regulating CXC chemokine ligand-12/receptor CXC chemokine receptor-4(CXCL12/CXCR4)signal pathway.Methods:10 rats were randomly selected as sham operation group,and the nucleus pulposus was placed on the top of the left L5 and L6 nerve roots.The 40 LDH rat model was constructed,and was grouped into model group,drug group(200 mg/kg total flavonoids of Eucommia ulmoides),activator group(5 μg/kg recombinant CXCL-12 protein),and drug+activator group(200 mg/kg total flavonoids of Eucommia ulmoides+5 μg/kg recombinant CXCL-12 protein),10 rats in each group,sham operation group and model group were given the same amount of normal saline for 21 d.The sensitivity of rats to mechanical stimulation and thermal stimulation was measured; the levels of TNF-α and IL-1β were detected by ELISA kits; histopathological changes of spinal dorsal horn were observed by HE staining; immunofluorescence staining was applied to detect the activation of microglia in the dorsal horn of the spinal cord; Western Blot was applied to detect the levels of CXCL12/CXCR4 signal pathway related proteins.Results:The total flavonoids of Eucommia ulmoides can increase the paw withdrawal threshold(PWT)and thermal withdrawal latency(TWL)values of rats with LDH,and reduce the levels of IL-1β,TNF-α,Iba-1 positive microglia,CXCL12,CXCR4,and NLRP3 proteins.After the treatment of total flavonoids of Eucommia ulmoides,the nerve bundles of rats were arranged orderly,and the infiltration of inflammatory cells was reduced.However,the trend of CXCL-12 treatment was opposite to that of Eucommia ulmoides total flavonoids treatment,and the anti-inflammatory and analgesic effects of Eucommia ulmoides total flavonoids on LDH rats were eliminated.Conclusion:Total flavonoids of Eucommia ulmoides may exert anti-inflammatory and analgesic effects on LDH rats by inhibiting CXCL12/CXCR4 signal pathway.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究课题(20220572) △通信作者 E-mail:t62yyl@163.com
更新日期/Last Update: 2025-04-10