[1]王俊龙,刘爱峰,张超△,等.温针灸对膝关节炎模型大鼠促炎因子及基质金属蛋白酶的影响[J].中国中医骨伤科杂志,2025,33(04):7-10+15.[doi:10.20085/j.cnki.issn1005-0205.250402 ]
　WANG Junlong,LIU Aifeng,ZHANG Chao,et al.Effects of Warm Needle Moxibustion on Pro-Inflammatory Factors and Matrix Metalloproteinases in Model Rats of Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(04):7-10+15.[doi:10.20085/j.cnki.issn1005-0205.250402 ]
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温针灸对膝关节炎模型大鼠促炎因子及基质金属蛋白酶的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年04期
页码:
7-10+15
栏目:
实验研究
出版日期:
2025-04-10

文章信息/Info

Title:
Effects of Warm Needle Moxibustion on Pro-Inflammatory Factors and Matrix Metalloproteinases in Model Rats of Knee Osteoarthritis
文章编号:
1005-0205(2025)04-0007-04
作者:
王俊龙1; 刘爱峰1; 张超1△; 李远栋1; 周鑫1; 张晓宇1; 张良1; 崔庆同1
1天津中医药大学第一附属医院(国家中医药管理局区域中医骨伤科诊疗中心,国家中医针灸临床医学研究中心)(天津,300381)
Author(s):
WANG Junlong1; LIU Aifeng1; ZHANG Chao1△; LI Yuandong1; ZHOU Xin1; ZHANG Xiaoyu1; ZHANG Liang1; CUI Qingtong1
1First Teaching Hospital of Tianjin University of Traditional Chinese Medicine(Regional Traditional Chinese Medicine Bone Injury Diagnosis and Treatment Center of State Administration of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion),Tianjin 300381,China.
关键词:
温针灸;  膝骨性关节炎;  白细胞介素-1β;  肿瘤坏死因子-α;  基质金属蛋白酶-13
Keywords:
warm needle moxibustion;  knee osteoarthritis;  interleukin-1β;  tumor necrosis factor-α;  matrix metalloproteinase-13
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250402
文献标志码:
A
摘要:
目的:观察温针灸对膝骨关节炎SD模型大鼠白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶-13(MMP-13)的影响,探讨温针灸治疗膝骨关节炎的作用机制。方法:30只SD雌性大鼠按随机数字表法随机分为空白组、模型组和观察组(每组10只)。空白组正常饲养,模型组与观察组采用Hulth法造模建立膝骨关节炎模型。造模结束后,观察组针刺联合艾灸患膝特定穴——足三里(EX-LE4)、犊鼻(ST35)和阳陵泉(GB34),每个穴位10 min/次,1次/d,共2周; 空白组及模型组正常饲养,不予任何干预。治疗结束后,采用酶联免疫吸附试验(ELISA)法检测关节滑膜组织中IL-1β、TNF-α含量,实时PCR检测法检测关节软骨中MMP-13含量。结果:与空白组相比,模型组及观察组滑膜组织中IL-1β、TNF-α的含量明显增多,差异有统计学意义(P<0.05); 且关节软骨中MMP-13的表达水平也显著升高,差异有统计学意义(P<0.05); 与模型组相比,观察组中各指标表达水平均显著降低,差异有统计学意义(P<0.01)。结论:温针灸能抑制IL-1β、TNF-α等炎性因子及MMP-13的表达,缓解关节炎症反应,减少关节软骨破坏,这可能是温针灸治疗膝骨关节炎的作用机制之一。
Abstract:
Objective:To observe the effects of warm needle moxibustion on interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and matrix metalloproteinase-13(MMP-13)in SD rats with knee osteoarthritis(KOA),and to explore the mechanism of warm needle moxibustion in the treatment of KOA.Methods:30 SD female rats were randomly divided into blank group,model group and observation group(10 rats in each group).The blank group was normally fed,and the model group and the observation group were modeled by Hulth method to establish the KOA model.After the modeling,the observation group acupuncture combined with moxibustion was specific to the affected knee:Zusanli(EX-LE4),calf nose(ST35)and Yanglingquan(GB34),each acupuncture point was 10 min/times,1 times/d,a total of 2 weeks,and the blank group and the model group were kept normally without any intervention.After treatment,enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of IL-1β and TNF-α in joint synovial tissue,and the content of MMP-13 in articular cartilage was detected by real-time PCR.Results:Compared with the blank group,the contents of IL-1β and TNF-α in the synovial tissues of the model group and the observation group were significantly increased(P<0.05).The expression level of MMP-13 in articular cartilage was also significantly increased(P<0.05).Compared with the model group,the expression level of each index in the observation group was significantly lower(P<0.01).Conclusion:Warm needle moxibustion can inhibit the expression of inflammatory factors such as IL-1β and TNF-α and MMP-13,alleviate joint inflammation and reduce joint cartilage destruction,which may be one of the mechanisms of warm needle moxibustion in the treatment of KOA.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82374489,81873316,81673994) 天津中医药大学第一附属医院 “拓新工程”基金科研课题(院YB202120) △通信作者 E-mail:zhangchao2004.love@163.com
更新日期/Last Update: 2025-04-10