[1]沈润斌,张倩,王旭洋,等.芍药苷促进骨折大鼠血管生成和骨折愈合的机制研究[J].中国中医骨伤科杂志,2025,33(03):12-17.[doi:1005-0205(2025)03-0012-06]
　SHEN Runbin,ZHANG Qian,WANG Xuyang,et al.Mechanism of Paeoniflorin Promoting Angiogenesis and Fracture Healing in Fractured Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(03):12-17.[doi:1005-0205(2025)03-0012-06]
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芍药苷促进骨折大鼠血管生成和骨折愈合的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年03期
页码:
12-17
栏目:
实验研究
出版日期:
2025-03-15

文章信息/Info

Title:
Mechanism of Paeoniflorin Promoting Angiogenesis and Fracture Healing in Fractured Rats
文章编号:
10.20085/j.cnki.issn1005-0205.250303
作者:
沈润斌1;  张倩1;  王旭洋1;  李国梁1;  马世强1;  苑翠翠1;  靳涛1;  韩广普1
1河北省沧州中西医结合医院(河北 沧州,061000)
Author(s):
SHEN Runbin1;  ZHANG Qian1;  WANG Xuyang1;  LI Guoliang1;  MA Shiqiang1;  YUAN Cuicui1;  JIN Tao1;  HAN Guangpu1
1Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine in Hebei Province,Cangzhou 061000,Hebei China.
关键词:
芍药苷;  缺氧诱导因子-1α/血管内皮生长因子;  骨折愈合;  血管生成
Keywords:
paeoniflorin;  hypoxia-inducible factor-1α/vascular endothelial growth factor;  fracture healing;  angiogenesis
分类号:
R-33
DOI:
1005-0205(2025)03-0012-06
文献标志码:
A
摘要:
目的:探究芍药苷调节缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)信号通路对骨折大鼠血管生成和骨折愈合的影响。方法:构建胫骨骨折大鼠模型,将骨折模型大鼠随机分为模型组、低剂量芍药苷组、高剂量芍药苷组、联合干预组。其中低、高剂量芍药苷组分别腹腔注射5,20 mg/kg芍药苷,联合干预组腹腔注射20 mg/kg芍药苷+100 mg/kg的PX-478(HIF-1α/VEGF通路抑制剂),持续给药4周,每组各6只。同批鼠中随机选取6只健康大鼠(只做右下肢切开伤口缝合不锯断胫骨)作为假手术组,假手术组和模型组注射等量的生理盐水。ELISA法检测血清中的碱性磷酸酶(ALP)、钙、磷水平; X射线检测胫骨骨折愈合程度; 生物力学实验检测大鼠相关指标; 苏木精-伊红(HE)检测骨折部位血管生成情况; Western Blot法检测HIF-1α及VEGF蛋白。结果:与假手术组相比,模型组大鼠胫骨有明显的骨折线,较多成熟骨组织,骨小梁排列呈网状,血清中碱性磷酸酶、磷、钙水平、最大载荷、刚度、血管数量、血管面积及HIF-1α、VEGF蛋白表达均明显降低(P<0.05); 与模型组相比,低/高剂量芍药苷组骨折线能达到完全愈合程度,骨小梁结构变紧密,骨质增厚,骨的连续性变好,血清中碱性磷酸酶、磷、钙水平、最大载荷、刚度、血管数量、血管面积及HIF-1α、VEGF蛋白表达均明显升高(P<0.05); 联合干预组则逆转上述变化。结论:芍药苷可能通过激活HIF-1α/VEGF通路,促进血管形成及骨折部位钙盐沉积,进而促进骨折愈合。
Abstract:
Objective:To investigate the effect of paeoniflorin on angiogenesis and fracture healing in rats with fractures by regulating hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:A rat model of tibial fracture was constructed,and the rats with fracture were randomly separated into model group,low dose paeoniflorin group,high dose paeoniflorin group and joint intervention group.Among them,the low dose paeoniflorin group and the high dose paeoniflorin group were intraperitoneally injected with 5 and 20 mg/kg paeoniflorin,respectively,and the joint intervention group was intraperitoneally injected with 20 mg/kg paeoniflorin + 100 mg/kg PX-478(HIF-1α/VEGF pathway inhibitor)for 4 weeks,with 6 rats in each group.6 healthy rats(only the right lower limb incision wound and suture without sawing off the tibia)were randomly selected as the sham group,and the sham group and the model group were injected with the same amount of normal saline.ELISA was used to detect the levels of alkaline phosphatase(ALP),calcium,phosphorus and other levels in serum.X-ray was used to detect the degree of healing of tibial fractures.Biomechanical experiments were conducted to detect the relevant indexes of rats.HE was used to detect angiogenesis at the fracture site.Western Blot was used to detect HIF-1α and VEGF proteins.Results:Compared with the sham group,the model group had obvious fracture lines in the tibia,more mature bone tissue,and the trabeculae were arranged in a network,the levels of ALP,phosphorus,calcium in serum,maximum load,stiffness,number of blood vessels,blood vessel area,and the protein expressions of HIF-1α and VEGF were obviously reduced(P<0.05).Compared with the model group,the fracture line of the low dose paeoniflorin group and the high dose paeoniflorin group could reach the degree of complete healing,the beam structure became tighter,the bone thickened,and the continuity of the bone became better,the levels of ALP,phosphorus,calcium in serum,maximum load,stiffness,number of blood vessels,blood vessel area,and the protein expressions of HIF-1α and VEGF were obviously increased(P<0.05).The joint intervention group reversed these changes.Conclusion:Paeoniflorin may promote angiogenesis and calcium salt deposition at the fracture site by activating the HIF-1α/VEGF pathway,thereby promoting fracture healing.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2018491)
更新日期/Last Update: 2025-03-10