[1]魏峰,岳树锦,陈婧,等.柚皮苷结合骨髓间充质干细胞对软骨下骨重塑的影响[J].中国中医骨伤科杂志,2025,33(03):7-11+17.[doi:1005-0205(2025)03-0007-05]
　WEI Feng,YUE Shujin,CHEN Jing,et al.Effects of Naringin Combined with Bone Marrow Mesenchymal Stem Cells on Subchondral Bone Remodeling[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(03):7-11+17.[doi:1005-0205(2025)03-0007-05]
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柚皮苷结合骨髓间充质干细胞对软骨下骨重塑的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年03期
页码:
7-11+17
栏目:
实验研究
出版日期:
2025-03-15

文章信息/Info

Title:
Effects of Naringin Combined with Bone Marrow Mesenchymal Stem Cells on Subchondral Bone Remodeling
文章编号:
10.20085/j.cnki.issn1005-0205.250302
作者:
魏峰1;  岳树锦2;  陈婧3;  李鹏洋1;  赵久丽1;  周连新1;  韩志华1;  权英1;  叶超1△
1北京中医药大学东直门医院(北京,100700)
2北京中医药大学护理学院
3北京中医药大学第三附属医院
Author(s):
WEI Feng1;  YUE Shujin2;  CHEN Jing3;  LI Pengyang1;  ZHAO Jiuli1;  ZHOU Lianxin1;  HAN Zhihua1;  QUAN Ying1;  YE Chao1△
1Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China; 2School of Nursing,Beijing University of Chinese Medicine,Beijing 102401,China; 3Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China.
关键词:
膝骨关节炎;  软骨下骨;  柚皮苷;  微计算机断层扫描技术;  骨髓间充质干细胞
Keywords:
knee osteoarthritis;  subchondral bone;  naringin;  Micro-CT scanning technique;  bone marrow mesenchymal stem cells
分类号:
R-33
DOI:
1005-0205(2025)03-0007-05
文献标志码:
A
摘要:
目的:基于Micro-CT扫描技术,观察柚皮苷结合骨髓间充质干细胞(BMSCs)对兔膝关节软骨下骨重塑的影响。方法:将兔骨髓间充质干细胞进行体外增殖,兔膝关节进行关节损伤造模,将骨髓间充质干细胞植入关节损伤处,并以柚皮苷汤或去离子水灌胃,分为假手术组、模型组、骨髓间充质干细胞组、柚皮苷结合骨髓间充质干细胞组,于第12周后对修复组织进行Micro-CT扫描检测(以软骨损伤修复区域缺损处为中心,数据三维重建后,通过分析软件CT Analyser量化数据),苏木精-伊红(HE)染色,X型胶原免疫组化染色。结果:从骨体积分数、骨小梁厚度及骨小梁数量看,柚皮苷结合骨髓间充质干细胞组高于模型组,且差异有统计学意义(P<0.05)。从骨表面积骨体积比及骨小梁分离度看,柚皮苷结合骨髓间充质干细胞组低于模型组和骨髓间充质干细胞组,且差异有统计学意义(P<0.05)。膝关节软骨下骨Micro-CT检测三维影像学比较,模型组的骨小梁明显减少,骨髓间充质干细胞组的骨小梁分布欠规律,柚皮苷结合骨髓间充质干细胞组的骨小梁数量增多,分布较密集,排列较规整。从HE染色看,柚皮苷结合骨髓间充质干细胞组可见软骨下骨修复处的骨小梁数量及密度增加,排列清晰。从X型胶原免疫组化看,柚皮苷结合骨髓间充质干细胞组可见关节软骨损伤修复处的X型胶原表达明显减少。结论:骨髓间充质干细胞具有促进兔膝关节损伤软骨下骨重塑的作用,加用柚皮苷能提高软骨下骨重塑的质量和数量,减少关节软骨X型胶原的表达,该效果的产生可能与柚皮苷激活TGF-β/Smad信号通路,促进骨髓间充质干细胞在软骨下骨处成骨方向的分化有关。
Abstract:
Objective:Based on micro-CT scanning technology,the effect of naringin combined with bone marrow mesenchymal stem cells(BMSCs)on subchondral bone remodeling was observed.Methods:Between rabbit bone marrow mesenchymal stem cells in vitro proliferation,the rabbit knee joint defect building,planted bone marrow mesenchymal stem cells joint defect,and with naringin soup or deionized water to fill the stomach,to set up sham operation group,model group,bone marrow mesenchymal stem cells group,naringin between bone marrow mesenchymal stem cells group,in the 12 weeks to repair tissue for Micro-CT scans,and look for areas of interest under the ROI through setting up the quantitative data.Results:In terms of BV/TV,Tb.Th and Tb.N,naringin between bone marrow mesenchymal stem cells group was higher than model group,and the difference was statistically significant(P<0.05).In terms of BS/BV,Tb.Sp,naringin between bone marrow mesenchymal stem cells group was lower than model group and bone marrow mesenchymal stem cells group,and the difference was statistically significant(P<0.05).According to three-dimensional imaging comparison of knee subchondral bone Micro-CT,bone trabeculae in the model group were significantly reduced,while bone trabeculae in the bone marrow mesenchymal stem cells group were not regularly distributed,while bone trabeculae in the naringin between bone marrow mesenchymal stem cells group were increased,more densely distributed,and more orderly arranged.HE staining showed that naringin between bone marrow mesenchymal stem cells group increased the number and density of bone trabeculae in subchondral bone repair,and the arrangement was clear.According to the immunohistochemistry of type X collagen,naringin between bone marrow mesenchymal stem cells group showed a significant decrease in the expression of type X collagen at the repair site of articular cartilage injury.Conclusion:BMSCs can promote subchondral bone remodeling in rabbit knee defect.The addition of naringin can improve the quality and quantity of subchondral bone remodeling,reduce the expression of type X collagen of articular cartilage.This effect may be related to naringin activation TGF-β/Smad signaling pathway.Stimulation is associated with promoting osteogenic differentiation of bone marrow mesenchymal stem cells at subchondral bone defects.

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备注/Memo

备注/Memo:
基金项目:中央高校基本科研业务费专项基金项目(2018-JYBZZ-JS091,2015-JYB-JSMS058)
国家自然科学基金青年科学基金项目(82104710)
△通信作者 E-mail:yechao@bucm.edu.cn
更新日期/Last Update: 2025-03-10