[1]白登彦△,张海军,王冠,等.葛根素对去卵巢大鼠骨代谢的影响机制研究[J].中国中医骨伤科杂志,2025,33(01):13-16+21.[doi:10.20085/j.cnki.issn1005-0205.250103]
　BAI Dengyan,ZHANG Haijun,WANG Guan,et al.The Mechanism of the Effect of Puerarin on Bone Metabolism in Ovariectomized Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(01):13-16+21.[doi:10.20085/j.cnki.issn1005-0205.250103]
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葛根素对去卵巢大鼠骨代谢的影响机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年01期
页码:
13-16+21
栏目:
实验研究
出版日期:
2025-01-15

文章信息/Info

Title:
The Mechanism of the Effect of Puerarin on Bone Metabolism in Ovariectomized Rats
文章编号:
1005-0205(2025)01-0013-04
作者:
白登彦1; 2△; 张海军1; 王冠1; 朱涛1; 赵志鹏1; 邓发军3
1甘肃省第二人民医院(兰州,730015)
2西北民族大学附属医院
3甘肃省和政县人民医院
Author(s):
BAI Dengyan1; 2△; ZHANG Haijun1; WANG Guan1; ZHU Tao1; ZHAO Zhipeng1; DENG Fajun3
1The Second People's Hospital of Gansu Province,Lanzhou 730015,China; 2Affiliated Hospital of Northwest University for Nationalities,Lanzhou 730015,China; 3Gansu Hezheng County People's Hospital,Hezheng 731299,Gansu China.
关键词:
葛根素;  丝裂原活化蛋白激酶信号通路;  去卵巢大鼠;  骨细胞增殖;  骨质疏松症
Keywords:
puerarin;  MAPK signaling pathway;  ovariectomy rats;  proliferation of bone cells;  osteoporosis
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250103
文献标志码:
A
摘要:
目的:探究葛根素通过调控丝裂原活化蛋白激酶(MAPK)信号通路对去卵巢大鼠骨细胞增殖及分化的影响机制。方法:将成年雌性SD大鼠随机分为假手术组(Sham组,仅切除卵巢周围的脂肪组织)和去卵巢组(手术摘除双侧卵巢)。去卵巢组大鼠进一步分为去卵巢手术组(OVX组)和葛根素治疗组(Pueraria组)。葛根素治疗组通过胃内给药方式治疗,持续8周。通过检测各组大鼠骨密度及骨的生物力学性能,分析血清中骨代谢标志物(TRAP和ALP)及炎性因子(TNF-α、IL-1β、IL-6)等指标变化,探究葛根素对骨细胞增殖、分化及炎性因子的影响。采用Western Blot和qRT-PCR技术检测丝裂原活化蛋白激酶信号通路相关蛋白和基因的表达。结果:与假手术组相比,去卵巢手术组大鼠骨密度及骨的生物力学性能下降,血清碱性磷酸酶(ALP)活性降低,抗酒石酸酸性磷酸酶(TRAP)活性增加,炎性因子升高,差异有统计学意义(P<0.05),表明骨质疏松症模型建立成功。葛根素治疗组大鼠骨密度及骨的生物力学性能提高,血清碱性磷酸酶活性增加,抗酒石酸酸性磷酸酶活性降低,炎性因子降低,差异有统计学意义(P<0.05),表明葛根素对骨细胞增殖及分化有显著影响。Western Blot和qRT-PCR技术检测结果显示,葛根素治疗组大鼠ERK1/2和p38-MAPK信号通路相关蛋白和基因的表达显著上调(P<0.05)。结论:葛根素通过调控丝裂原活化蛋白激酶信号通路,显著促进去卵巢大鼠骨细胞的增殖和成骨分化,抑制破骨细胞的活性,从而改善骨质疏松症状。
Abstract:
Objective:To investigate the mechanism by which puerarin regulates the mitogen activated protein kinase(MAPK)signaling pathway on the proliferation and differentiation of bone cells in ovariectomized rats.Methods:Adult female SD rats were randomly divided into sham surgery group(sham group,only removing adipose tissue around the ovaries)and ovariectomy group surgically removing both ovaries).The rats in the ovariectomy group were further divided into OVX group and pueraria group treated with puerarin.The puerarin group was treated by gastric administration for 8 weeks.By detecting the bone density and biomechanical properties of each group of rats,analyzing the changes in serum alkaline phosphatase(ALP),tartrate resistant acid phosphatase(TRAP),inflammatory factors,and other indicators,we aimed to explore the effects of puerarin on bone cell proliferation,differentiation,and inflammatory factors.Western Blot and qRT-PCR techniques were used to detect the expression of MAPK signaling pathway related proteins and genes.Results:Compared with the sham group,the bone density and biomechanical properties of the OVX group rats decreased,serum ALP activity decreased,TRAP activity increased,and inflammatory factors increased,indicating the successful establishment of the osteoporosis model(P<0.05).The bone density and biomechanical properties of Pueraria group rats increased,serum ALP activity increased,TRAP activity decreased,and inflammatory factors decreased,indicating that puerarin had a significant effect on bone cell proliferation and differentiation(P<0.05).Western Blot and qRT-PCR results showed that the expression of ERK1/2 and p38 MAPK signaling pathway related proteins and genes was significantly upregulated in Pueraria group rats(P<0.05).Conclusion:Puerarin significantly promotes the proliferation and osteogenic differentiation of bone cells in ovariectomized rats by regulating the MAPK signaling pathway,inhibits the activity of osteoclasts,and thus improves osteoporosis symptoms.

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备注/Memo

备注/Memo:
基金项目:甘肃省兰州市科技计划项目(2022-ZD-56)
甘肃省临夏州科技项目(2022BS2016)
△通信作者 E-mail:dawanyule@foxmail.com
更新日期/Last Update: 2025-01-15