[1]段飞△,郑盼盼,朱以海,等.兔髌骨全切除术后膝关节退变研究[J].中国中医骨伤科杂志,2025,33(01):1-6.[doi:10.20085/j.cnki.issn1005-0205.250101]
　DUAN Fei,ZHENG Panpan,ZHU Yihai,et al.A Study about Knee Joint Degeneration after Total Patellectomy of Rabbits[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(01):1-6.[doi:10.20085/j.cnki.issn1005-0205.250101]
点击复制

兔髌骨全切除术后膝关节退变研究()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年01期
页码:
1-6
栏目:
实验研究
出版日期:
2025-01-15

文章信息/Info

Title:
A Study about Knee Joint Degeneration after Total Patellectomy of Rabbits
文章编号:
1005-0205(2025)01-0001-06
作者:
段飞1; 2△; 郑盼盼2; 朱以海2; 李文省2; 王炳晨2; 孙全波2; 蒋冬升2; 庞永冰2; 李阵林2; 肖善文2
1菏泽医学专科学校附属医院(山东 菏泽,274000)
2菏泽医学专科学校
Author(s):
DUAN Fei1; 2△; ZHENG Panpan2; ZHU Yihai2; LI Wenxing2; WANG Bingchen2; SUN Quanbo2; JIANG Dongsheng2; PANG Yongbing2; LI Zhenlin2; XIAO Shanwen2
1Affiliated Hospital of Heze Medical College,Heze 274000,Shandong China; 2Heze Medical College,Heze 274000,Shandong China.
关键词:
兔髌骨全切除术;  新生骨;  股骨滑车;  膝关节;  半月板
Keywords:
total patellectomy in rabbits;  new bone formation;  femoral trochlear;  knee joint;  meniscus
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250101
文献标志码:
A
摘要:
目的:研究兔髌骨全切除术后膝关节形态及病理改变。方法:20只新西兰大白兔随机分为空白组、对照组和观察组,其中观察组大白兔膝关节行髌骨全切除术。1年后处死动物,切取髌韧带、股骨滑车、股骨和胫骨关节面软骨和半月板,并观察形态学及组织学改变; 半月板切片苏木精-伊红染色,通过ImageJ图像处理分析软件定量分析半月板软骨陷窝深染的老化细胞面积。结果:空白组与对照组的股骨滑车沟深度和角度、Mankin评分、半月板软骨陷窝深染的老化细胞面积,差异无统计学意义(P>0.05)。观察组原髌骨所在的髌韧带无新生骨形成,股骨滑车软骨细胞病理表现相较于空白组和对照组退变明显,股骨滑车沟角度、Mankin评分及半月板软骨陷窝深染的老化细胞面积明显大于空白组和对照组,差异有统计学意义(P<0.05)。结论:髌骨全切除术后髌韧带无软骨再生,股骨滑车沟变浅,软骨及半月板退变加速,对后期可能进行的膝关节置换术影响深远,因此应慎重选择髌骨全切除术。
Abstract:
Objective:To investigate the morphology and pathological changes of the knee joint after total patellar resection in rabbits.Methods:20 New Zealand white rabbits were randomly divided into blank group,control group and observation group,and the observation group underwent total patellar resection of the knee joint.One year later,the animals were euthanized and the patellar ligament,femoral trochlear,femoral and tibial articular cartilage,and meniscus were harvested,and morphological and histological changes were observed.The hematoxylin eosin staining of meniscus slices was used to quantitatively analyze the aging cell area of deep stained meniscus cartilage pits using ImageJ image processing analysis software.Results:There was no statistically significant difference(P>0.05)in the depth and angle of the femoral trochlear groove,Mankin score,and the area of aged cells with deep staining of meniscus cartilage pits between the blank group and the control group.There was no new bone formation in the patellar ligament where the original patella was located in the observation group.The pathological manifestations of femoral trochlear chondrocytes showed significant degeneration compared to the blank group and control group.The angle of the femoral trochlear groove,Mankin score,and the area of deeply stained aging cells in the meniscus cartilage fossa were significantly larger than those in the blank group and the control group,and the difference was statistically significant(P<0.05).Conclusion:After total patellar resection,there is no cartilage regeneration in the patellar ligament,the trochlear groove of the femur becomes shallower,and the degeneration of cartilage and meniscus accelerates,which has a profound impact on the possible knee replacement surgery in the later stage.Therefore,total patellar resection should be carefully selected.

参考文献/References:

[1] STEINMETZ S,BRÜGGER A,CHAUVEAU J,et al.Practical guidelines for the treatment of patellar fractures in adults[J].Swiss Med Wkly,2020,150:w20165.
[2] SHARAFATVAZIRI A,ESFANDIARI S,VOSOUGHI F,et al.Patellectomy,an old treatment in patellar fracture which is still alive:a case report[J].Int J Surg Case Rep,2024,118:109604.
[3] HIEMSTRA L A,FRIZZELL B,KERSLAKE S.Medial quadriceps tendon femoral ligament reconstruction after patellectomy:a treatment for a dislocating quadriceps tendon[J].J Am Acad Orthop Surg Glob Res Rev,2017,1(1):e001.
[4] DENG X,ZHU L,HU H,et al.Comparison of total patellectomy and osteosynthesis with tension band wiring in patients with highly comminuted patella fractures:a 10-20-year follow-up study[J].J Orthop Surg Res,2021,16(1):497.
[5] CHEN H,LU H,HUANG J,et al.Calcitonin gene-related peptide influences bone-tendon interface healing through osteogenesis:investigation in a rabbit partial patellectomy model[J].Orthop J Sports Med,2021,9(7):23259671211003982.
[6] 王琳,吕红斌,霍碧君,等.兔髌骨-髌腱结合部新骨形成与大小预测其愈合程度[J].医用生物力学,2006,21(4):291-297.
[7] 陈士文,路屹,陈传好,等.兔膝关节解剖学特点及在动物模型的意义[J].中国临床解剖学杂志,2012,30(1):100-102.
[8] MITCHELL N,SHEPARD N.Effect of patellar shaving in the rabbit[J].J Orthop res,1987,5(3):388-392.
[9] QIN L,LEUNG K S,CHAN C W,et al.Enlargement of remaining patella after partial patellectomy in rabbits[J].Med Sci Sports Exerc,1999,31(4):502-506.
[10] LU J F,WANG C H,LI F Q,et al.Changes in cartilage and subchondral bone of femoral trochlear groove after patellectomy in growing rabbits[J].Orthop Surg,2020,12(2):653-660.
[11] PUTZ R,BOSZCZYK B,MILZ S.How the ends of bones evolve and what they do:the anatomical and biomechanical perspective[J].Semin Musculoskelet Radiol,2019,23(5):467-476.
[12] TURNER C H.Biomechanics of bone:determinants of skeletal fragility and bone quality[J].Osteoporos Int,2002,13(2):97-104.
[13] PARANJAPE C S,CUTCLIFFE H C,GRAMBOW S C,et al.A new stress test for knee joint cartilage[J].Sci Rep,2019,9(1):2283.
[14] LEUNG K S,QIN L,LEUNG M C,et al.Partial patellectomy induces a decrease in the proteoglycan content in the remaining patellar articular cartilage:an experimental study in rabbits[J].Clin Exp Rheumatol,1999,17(5):597-600.
[15] CUZZUPOLI F,BOLTRI F.Anatomical,clinical and surgical features of total and partial patellectomy in the treatment of comminuted fractures of the kneecap[J].Arch Sci Med(Torino),1969,126(3):93-99.
[16] MILGRAM J W.Injury to articular cartilage joint surfaces.I.Chondral injury produced by patellar shaving:a histopathologic study of human tissue specimens[J].Clin Orthop Relat Res,1985,192:168-173.
[17] 李旭,李石伦,李升,等.全切术和部分切除术对髌骨粉碎性骨折的远期疗效比较[J].河北医药,2020,42(12):1809-1812.
[18] MASLOW J,ZUCKERMAN J D,IMMERMAN I.Total knee arthroplasty in patients with a previous patellectomy[J].Bull Hosp Jt Dis,2013,71(3):227-230.
[19] DODDS A,CROWLEY R,MENZ T,et al.Outcome following total knee replacement in patients with a previous patellectomy[J].Acta Orthop Belg,2018,84(3):251-256.
[20] 郭帅成,黄健.膝关节骨性关节炎治疗研究进展[J].内蒙古医科大学学报,2021,43(3):320-323.
[21] 杨昆,周游,梁朝鑫,等.富血小板血浆在创面修复的研究进展[J].中国骨与关节杂志,2024,13(6):471-475.
[22] 李海群,张志仕,李博,等.医用臭氧水对兔膝关节骨性关节炎软骨IL-1β、TNF-α与VEGF表达的影响[J].解剖科学进展,2018,24(1):12-14.

备注/Memo

备注/Memo:
基金项目:山东省医药卫生科技发展计划项目(202204070831)
△通信作者 E-mail:670019131@qq.com
更新日期/Last Update: 2025-01-15