[1]吕权,高金金,杨玉林,等.姿势性应力诱导椎间盘退变动物模型的实验研究[J].中国中医骨伤科杂志,2024,32(12):10-14+19.[doi:10.20085/j.cnki.issn1005-0205.241202 ]
　L Quan,GAO Jinjin,YANG Yulin,et al.Experimental Study on Animal Model of Spontaneous Intervertebral Disc Degeneration Induced by Postural Stress[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(12):10-14+19.[doi:10.20085/j.cnki.issn1005-0205.241202 ]
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姿势性应力诱导椎间盘退变动物模型的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年12期
页码:
10-14+19
栏目:
实验研究
出版日期:
2024-12-15

文章信息/Info

Title:
Experimental Study on Animal Model of Spontaneous Intervertebral Disc Degeneration Induced by Postural Stress
文章编号:
1005-0205(2024)12-0010-05
作者:
吕权1; 2; 高金金3; 杨玉林3; 王磊2; 孙甫1; 2△
1西安医学院第一附属医院(西安,710077)
2宁夏医科大学第一临床医学院
3西安医学院
Author(s):
LÜ Quan1; 2; GAO Jinjin3; YANG Yulin3; WANG Lei2; SUN Fu1; 2△
1First Affiliated Hospital of Xi'an Medical College,Xi'an 710077,China; 2First Clinical School of Ningxia Medical University,Yinchuan 750004,China; 3Xi'an Medical College,Xi'an 710077,China.
关键词:
椎间盘退变;  椎体失稳;  髓核;  髓核细胞;  纤维环
Keywords:
intervertebral disc degeneration;  vertebral instability;  nucleus pulposus;  nucleus pulposus cells;  fiber ring
分类号:
R681.5
DOI:
10.20085/j.cnki.issn1005-0205.241202
文献标志码:
A
摘要:
目的:分析椎间盘的退变机制,建立一种模仿人体姿势性应力诱发的椎间盘退变模型。方法:建立大鼠椎间盘纤维环穿刺及姿势性应力诱导椎间盘退变模型,将大鼠分为3组,每组10只。对照组不做处理,常规饲养; 纤维环穿刺组使用16号针穿刺大鼠椎间盘纤维环建立模型; 姿势性应力诱导椎间盘退变组将大鼠放置于大小适中的方形透明塑料管内,将管体倾斜60°放置,每日持续8 h,使大鼠在倾斜面上活动或休息,改变大鼠椎体应力,建立模型。分别在第6周和第12周观察大鼠苏木精-伊红(HE)染色、Masson染色和免疫组化结果。结果:建立了大鼠椎间盘纤维环穿刺及姿势性应力诱导椎间盘退变模型。HE染色结果显示:纤维环针刺组与姿势性应力诱导椎间盘退变组髓核细胞数量均较对照组减少,且随着时间的推移减少越发明显。Masson染色结果显示:与对照组相比,纤维环针刺组纤维环结构被破坏,纤维环与髓核边界不清; 姿势性应力诱导椎间盘退变组纤维环结构紊乱,向两侧明显突出,髓核与纤维环边界不清。免疫组化结果显示:与对照组相比,第6周时,纤维环针刺组和姿势性应力诱导椎间盘退变组纤维环Ⅱ型胶原阳性细胞数量下降(P<0.05,P<0.01); 第12周时,纤维环针刺组和姿势性应力诱导椎间盘退变组仍表现出纤维环Ⅱ型胶原阳性细胞数量,姿势性应力诱导椎间盘退变组下降更为明显,但第6周与第12周对比,差异无统计学意义(P<0.05,P<0.01)。椎间盘退变评分显示:与对照组相比,第6周时,纤维环针刺组和姿势性应力诱导椎间盘退变组椎间盘发生退变(P<0.05,P<0.001); 第12周时,纤维环针刺组和姿势性应力诱导椎间盘退变组椎间盘退变更加明显(P<0.01,P<0.001)。结论:本实验通过无损伤姿势性应力诱导椎间盘退变成功建立椎间盘退变模型,该模型能够模仿人类直立行走的姿势,退变过程更接近人体结构,具有一定的合理性。
Abstract:
Objective:To study the mechanism of intervertebral disc degeneration and establish an intervertebral disc degeneration model that mimics human postural stress induction.Methods:The rat models of intervertebral disc degeneration induced by fibrous ring puncture and postural stress were establish and divide into three groups,with 10 rats in each group.Control group:No treatment,conventional feeding.Annulus fibrosus puncture(AFP)group:A model was established by puncturing the fibrous ring of the intervertebral disc in rats using a 16th needle.Postural stress induced intervertebral disc degeneration group:Rats were placed in a moderately sized square transparent plastic tube,the tube was tilted at a 60° angle for 8 h a day,and the rats were allowed to move or rest on the inclined surface to change the stress on the rat vertebral body and establish a model.The HE staining,Masson staining,and immunohistochemical results of rats were observed at 6 and 12 weeks,respectively.Results:The rat model of intervertebral disc degeneration induced by fibrous ring puncture and postural stress was established.HE staining results showed that the number of nucleus pulposus cells decreased in the fibrous ring puncture group and the postural stress induced intervertebral disc degeneration group compared to the control group,and the decrease became more pronounced over time.The Masson staining results showed that compared with the control group,the fibrous ring structure was disrupted in the fibrous ring acupuncture group,and the boundary between the fibrous ring and the nucleus pulposus was unclear; the fibrous ring structure in the intervertebral disc degeneration group induced by postural stress was disordered,protruded significantly to both sides,and the boundary between the nucleus pulposus and the fibrous ring was unclear.The immunohistochemical results showed that compared with the control group,the number of type Ⅱ collagen positive cells in the annulus fibrosus acupuncture group and the posture stress induced intervertebral disc degeneration group decreased at week 6(P<0.05,P<0.01).At week 12,the annulus fibrosus acupuncture group and the posture stress induced intervertebral disc degeneration group still showed the number of type Ⅱ collagen positive cells,with a more significant decrease in the posture stress induced intervertebral disc degeneration group.However,there was no statistically significant difference between week 6 and week 12(P<0.05,P<0.01).The intervertebral disc degeneration score showed that compared with the control group,the fibrous ring acupuncture group and the postural stress induced intervertebral disc degeneration group had intervertebral disc degeneration at week 6(P<0.05,P<0.001); at week 12,the fibrous ring acupuncture group and the postural stress induced intervertebral disc degeneration group had more significant intervertebral disc degeneration(P<0.01,P<0.001).Conclusion:This experiment successfully established an intervertebral disc degeneration model through non-invasive postural stress.This model has a certain degree of innovation and can mimic the posture of human upright walking.The degeneration process is closer to the structure of the human body and has a certain degree of rationality.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81371987)
西安医学院第一附属医院院内配套经费项目(XYFYPT-2020-05)
△通信作者 E-mail:sunfu20019@163.com
更新日期/Last Update: 2024-12-05