[1]郑阳,仇湘中△,黄承军,等.补肝健腰方对腰椎间盘突出髓核细胞基质金属蛋白酶3、蛋白聚糖及信使核糖核酸表达的影响[J].中国中医骨伤科杂志,2024,32(09):6-11.[doi:10.20085/j.cnki.issn1005-0205.240902]
　ZHENG Yang,QIU Xiangzhong,HUANG Chengjun,et al.Effect of Bugan Jianyao Decoction on the Expression of MMP-3,Aggrecan Protein and mRNA in Nucleus Pulpocytes of Lumbar Disc Herniation[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(09):6-11.[doi:10.20085/j.cnki.issn1005-0205.240902]
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补肝健腰方对腰椎间盘突出髓核细胞基质金属蛋白酶3、蛋白聚糖及信使核糖核酸表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年09期
页码:
6-11
栏目:
实验研究
出版日期:
2024-09-15

文章信息/Info

Title:
Effect of Bugan Jianyao Decoction on the Expression of MMP-3,Aggrecan Protein and mRNA in Nucleus Pulpocytes of Lumbar Disc Herniation
文章编号:
1005-0205(2024)09-0006-06
作者:
郑阳1; 仇湘中2△; 黄承军1; 陈中3; 张信成2
1柳州市中医医院(广西 柳州,545001)
2湖南省中医药研究院附属医院
3重庆市北碚区中医院
Author(s):
ZHENG Yang1; QIU Xiangzhong2△; HUANG Chengjun1; CHEN Zhong3; ZHANG Xincheng2
1Liuzhou Traditional Chinese Medical Hospital,Liuzhou 545001,Guangxi China;
2Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,China;
3Chongqing Beibei Traditional Chinese Medical Hospital,Chongqing 400799,China.
关键词:
补肝健腰方;  腰椎间盘突出;  髓核细胞;  基质金属蛋白酶-3;  蛋白聚糖
Keywords:
Bugan Jianyao decoction;  lumbar disc herniation;  nucleusted cells;  matrix metalloproteinase-3(MMP-3);  aggrecan
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.240902
文献标志码:
A
摘要:
目的:研究补肝健腰方对基质金属蛋白酶-3(MMP-3)、蛋白聚糖(Aggrecan)蛋白及mRNA表达的影响,从而探索其治疗腰椎间盘突出症可能的疗效靶点和作用机制。方法:20只大鼠随机分组后制备补肝健腰方含药血清和正常血清,将培养的人体髓核细胞进行分组干预。实验分为9组,即空白组,siRNA NC组,NF-κB p65 siRNA阻断组,5%,10%及20%体积分数正常血清组,5%,10%及20%体积分数补肝健腰方含药血清组,在24 h和48 h时采用实时定量逆转录聚合酶链式反应(RT-qPCR)检测各组髓核细胞 MMP-3、Aggrecan mRNA表达,采用蛋白质免疫印迹试验(Western Blot)检测各组 MMP-3、Aggrecan 蛋白表达。结果:正常血清组与补肝健腰方含药血清组比较,相同体积分数的补肝健腰方含药血清组MMP-3 mRNA转录和蛋白表达均明显降低,Aggrecan mRNA和蛋白表达明显升高(P<0.05),三组补肝健腰方含药血清组之间比较,以20%体积分数补肝健腰方含药血清MMP-3 的mRNA转录和其蛋白表达降低最多,Aggrecan mRNA和蛋白表达提升最多(P<0.05)。补肝健腰方对MMP-3、Aggrecan mRNA转录以及Aggrecan、MMP-3蛋白表达水平的影响在24 h和48 h两个检测时间点差异无统计学意义。结论:补肝健腰方的疗效与药物浓度有一定相关性,可能是通过抑制NF-κB p65信号通路达到治疗腰椎间盘突出症的效果。
Abstract:
Objective:To explore the effect of Bugan Jianyao decoction on the expression of matrix metalloproteinase-3(MMP-3),aggrecan protein and mRNA and explore its potential targets and mechanisms of action on the treatment of lumbar intervertebral disc herniation.Methods:20 rats were randomly divided into groups to prepare Bugan Jianyao decoction containing serum and normal serum; human nucleus pulposus cells(which had passed ethical review)were subjected interventions in groups.The cells were divided into 9 groups:blank group,siRNA NC group,NF-κB p65 siRNA inhibition group,5%,10%,and 20% normal serum group,and 5%,10%,and 20% serum containing the formula group.At 24 h and 48 h,RT-qPCR was used to detect the expression of MMP-3,aggrecan mRNA in each group of nucleus pulposus cells,and Western Blot was used to detect the expression of MMP-3,aggrecan protein in each group.Results:In terms of group:compared with the normal serum group,the same body weight of the serum containing the formula group showed a significant decrease in MMP-3 mRNA transcription and protein expression,and showed a significant increase in aggrecan mRNA and protein expression(P<0.05).Among the three groups of serum containing the formula,the 20% serum group showed the greatest decrease in MMP-3 mRNA transcription and protein expression,and the greatest increase in aggrecan mRNA and protein expression(P<0.05).In terms of time:the effect of Bugan Jianyao decoction on the expression of MMP-3,aggrecan mRNA,aggrecan and MMP-3 protein were not significantly different at the two detection time points of 24 h and 48 h.Conclusion:The efficacy of Bugan Jianyao decoction is related to the dose of the medicine,and it may achieve therapeutic effects on the treatment of lumbar intervertebral disc herniation by inhibiting the NF-κB p65 signaling pathway.

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备注/Memo

备注/Memo:
基金项目:国家中医药管理局“全国名老中医药专家仇湘中传承工作室建设项目”(国中医药人教〔2016〕42号)
广西壮族自治区卫生厅中医药科技专项课题(GZKZ-Z1106)
长沙市科技局项目(kq1901066,kq1907119)
湖南省中医药科研计划项目(202021)
△通信作者 E-mail:18974991366@189.cn
更新日期/Last Update: 2024-09-05