[1]郑含笑,谢先敏,王鹏,等.续筋接骨液促进急性肌腱损伤修复的机制研究[J].中国中医骨伤科杂志,2024,32(06):25-29.[doi:10.20085/j.cnki.issn1005-0205.240605]
　ZHENG Hanxiao,XIE Xianmin,WANG Peng,et al.Study of Mechanism of the Renewing Tendon and Bone Splice Liquid in Promoting the Repair of Acute Tendon Injury[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(06):25-29.[doi:10.20085/j.cnki.issn1005-0205.240605]
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续筋接骨液促进急性肌腱损伤修复的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年06期
页码:
25-29
栏目:
实验研究
出版日期:
2024-06-15

文章信息/Info

Title:
Study of Mechanism of the Renewing Tendon and Bone Splice Liquid in Promoting the Repair of Acute Tendon Injury
文章编号:
1005-0205(2024)06-0025-05
作者:
郑含笑1; 谢先敏2; 王鹏1; 李梓宸3△; 谢心军2△
1湖南中医药大学第一中医临床学院(长沙,410208); 2湖南中医药大学第一附属医院; 3湖北省中医院
Author(s):
ZHENG Hanxiao1; XIE Xianmin2; WANG Peng1; LI Zichen3△; XIE Xinjun2△
1First Clinical College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China; 2First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China; 3Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China.
关键词:
续筋接骨液;  肌腱损伤;  碱性成纤维细胞生长因子;  转化生长因子β1;  磷脂酰肌醇3激酶/蛋白激酶B信号通路
Keywords:
renewing tendon and bone splice liquid;  tendon healing;  transforming growth factor β1(TGF-β1);  basic fibroblast growth factor(bFGF);  PI3K-Akt-mTOR pathway
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.240605
文献标志码:
A
摘要:
目的:采用横行贯通切断大鼠跟腱纵轴制作急性肌腱损伤模型,用续筋接骨液灌胃治疗,探讨续筋接骨液影响肌腱愈合的作用机制。方法:将45只SD大鼠随机分为5组,分别为模型组、假手术组、续筋接骨液低、中、高剂量组,每组9只。假手术组切开皮肤后直接缝合皮肤,其余4组建立跟腱损伤模型并手术修复肌腱缝合皮肤。续筋接骨液低、中、高剂量组分别用3.12,6.25,12.50 mL/kg灌胃干预,模型组与假手术组用等量的生理盐水灌胃干预。4周后观察各组大鼠肌腱愈合情况,ELISA法检测各组大鼠血清中转化生长因子β1(TGF-β1)和碱性成纤维细胞生长因子(bFGF)的表达情况; RT-PCR法检测肌腱组织中磷脂酰肌醇-3激酶(PI3K)、蛋白激酶B(Akt)、雷帕霉素靶蛋白(mTOR)、TGF-β1及bFGF的表达水平。结果:与模型组比较,其余4组大鼠血清中TGF-β1和bFGF表达水平均降低,差异有统计学意义(P<0.05); 肌腱组织中TGF-β1、bFGF、PI3K、Akt及mTOR表达水平降低,差异有统计学意义(P<0.05)。与续筋接骨液低剂量组比较,续筋接骨液中、高剂量组大鼠血清TGF-β1和bFGF表达水平均降低,差异有统计学意义(P<0.05); 肌腱组织中TGF-β1、bFGF、PI3K、Akt及mTOR表达水平降低,差异有统计学意义(P<0.05); 续筋接骨液中、高剂量组之间各项指标差异无统计学意义(P>0.05)。与续筋接骨液高剂量组比较,假手术组大鼠血清中TGF-β1和bFGF表达水平降低,差异有统计学意义(P<0.05); 肌腱组织中TGF-β1、bFGF、PI3K、Akt及mTOR表达水平降低,差异有统计学意义(P<0.05)。结论:续筋接骨液在大鼠肌腱愈合过程中有促进作用,并可以减少肌腱粘连发生,其作用机制可能与其抑制TGFβ1、bFGF及PI3K-Akt-mTOR通路表达有关。
Abstract:
Objective:Acute tendon injury was modeled by transverse cut of the longitudinal axis of the achilles tendon in rats,and was treated by the renewing tendon and bone splice liquid to investigate the mechanism of the renewing tendon and bone splice liquid in affecting the healing of the tendon.Methods:45 SD rats were randomly divided into 5 groups:model group,sham operation group,low,medium and high dose group,with 9 rats in each group.In the sham operation group,the skin was directly sutured after incision,and the tendon injury models were established in other 4 groups and skin were sutured after tendon repair.The low,medium and high dose group were given 3.12,6.25,12.50 mL/kg of renewing tendon and bone splice liquid respectively,and model group and control group with the same amount of saline lavage intervention.The tendon healing of each group was observed 4 weeks later,and the expression of transforming growth factor β1(TGF-β1)and basic fibroblast growth factor(bFGF)in serum of each group was detected by ELISA.The expression levels of phosphatidylinositol-3 kinase(PI3K),protein kinase B(Akt)target of rapamycin(mTOR),TGF-β1 and bFGF were detected by RT-PCR.Results:Compared with model group,the expression levels of TGF-β1 and bFGF in serum of the other 4 groups were decreased(P<0.05),and the expression levels of TGF-β1,bFGF,PI3K,Akt and mTOR in tendon tissues were decreased(P<0.05).Compared with the low-dose group,the expression levels of TGF-β1 and bFGF in serum in the middle and high-dose groups were decreased(P<0.05),while the expression levels of TGF-β1,bFGF,PI3K,Akt and mTOR in tendon tissues in the middle and high-dose groups were decreased(P<0.05),and there were no significant differences in all indexes between the medium and high dose groups(P>0.05).The expression levels of TGF-β1 and bFGF in serum and TGF-β1,bFGF,PI3K,Akt and mTOR in tendon tissues were decreased in sham operation group compared with the high dose group(P<0.05).Conclusion:The renewing tendon and bone-setting liquid plays a promoting role in the process of muscle tendon healing,and can reduce tendon adhesion occurred.Its mechanism may be related to its inhibition of TGF-β1,bFGF,PI3K-Akt-mTOR pathway expression.

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备注/Memo

备注/Memo:
基金项目:湖南省自然科学基金项目(2023JJ40510)
湖南省卫健委科研课题(202104071648)
湖南省中医药科研项目(2021213)
湖南中医药大学院校联合基金项目(2022XYLH017)
△通信作者 E-mail:lizichen230@163.com(李梓辰)
更新日期/Last Update: 2024-06-15