[1]俞云飞 胡钢△ 蔡建平 严松鹤.补气活血通络方对大鼠创伤性深静脉血栓形成及血红素加氧酶-1基因表达的影响[J].中国中医骨伤科杂志,2021,29(09):5-9.
　YU Yunfei HU Gang CAI Jianping YAN Songhe.Efficacy of Buqi Huoxue Tongluo Decoction on Traumatic DeepVein Thrombosis and Hmox-1 Gene Expression in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(09):5-9.
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补气活血通络方对大鼠创伤性深静脉血栓形成及血红素加氧酶-1基因表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年09期
页码:
5-9
栏目:
实验研究
出版日期:
2021-09-15

文章信息/Info

Title:
Efficacy of Buqi Huoxue Tongluo Decoction on Traumatic DeepVein Thrombosis and Hmox-1 Gene Expression in Rats
文章编号:
1005-0205(2021)09-0005-05
作者:
俞云飞1 胡钢1△ 蔡建平1 严松鹤1
1江苏无锡市中医医院(江苏 无锡,214000)
△通信作者 E-mail:fantasyhg@163.com
Author(s):
YU Yunfei1 HU Gang1△ CAI Jianping1 YAN Songhe1
1Wuxi Hospital of Traditional Chinese Medicine,Wuxi 214000,Jiangsu China.
关键词:
补气活血通络方;  血红素加氧酶-1;  深静脉血栓
Keywords:
Buqi Huoxue Tongluo decoction;  Hmox-1;  deep vein thrombosis
分类号:
R-33
文献标志码:
A
摘要:
目的:观察补气活血通络方对大鼠创伤性深静脉血栓形成及血红素加氧酶-1(Hmox-1)基因表达的影响。方法:采用钳夹大鼠单侧股静脉并予后肢髋人字石膏固定法建立创伤性深静脉血栓模型。将36只SD大鼠随机分为造模组、低浓度中药组、高浓度中药组、阿司匹林组,造模24 h后分别使用生理盐水、补气活血通络方或阿司匹林肠溶片对大鼠灌服3 d后,检测股静脉血栓干、湿重。运用苏木精-伊红(HE)染色观察股静脉血管形态学改变及血栓情况,实时PCR检测Hmox-1基因的表达情况。结果:造模成功后肉眼可见股静脉血栓形成,HE染色见股静脉管腔内血栓形成。与造模组相比,高浓度中药组和阿司匹林组股静脉中血栓质量明显降低(P<0.05),HE染色镜下也呈现相同趋势; 而与造模组相比,低浓度和高浓度中药组中Hmox-1基因呈现浓度依赖性表达增高(P<0.05),但阿司匹林组中Hmox-1基因表达低于两个中药组。结论:补气活血通络方可能通过促进Hmox-1基因表达来降低大鼠创伤性深静脉血栓形成。
Abstract:
Objective:To observe the efficacy of Buqi Huoxue Tongluo decoction on traumatic deep vein thrombosis(DVT)and Hmox-1 gene expression in rats.Methods:The rat model of traumatic DVT was established by clamping the unilateral femoral vein and fixing the posterior hip with herringbone plaster.36 SD rats were randomly divided into model group,low-concentration Chinese medicine group,high-concentration Chinese medicine group and Aspirin group.24 h after model establishment,the rats were given normal saline,Buqi Huoxue Tongluo decoction or Aspirin enteric-coated tablet for 3 d respectively,and the dry and wet weight of femoral vein thrombus were detected.The morphological changes of femoral vein vessels and thrombosis were observed by Hematoxylin-Eosin(HE)staining.The expression of Hmox-1 gene was detected by RT-PCR.Results:Femoral vein thrombosis could be seen by naked eyes after the model was successfully established,and intraluminal thrombosis of femoral vein was observed by HE staining.Compared with the model group,the mass of thrombosis in the femoral vein in the high-concentration Chinese medicine group and the Aspirin group was significantly decreased(P<0.05),and the same trend was observed under HE staining microscope.Compared with the model group,the expression of Hmox-1 gene in low and high concentration Chinese medicine groups were increased in a concentration-dependent manner(P<0.05),but the expression of Hmox-1 gene in Aspirin group was lower than that in the two Chinese medicine groups.Conclusion:Buqi Huoxue Tongluo decoction may reduce traumatic DVT in rats by promoting Hmox-1 gene expression.

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备注/Memo

备注/Memo:
基金项目:无锡市卫计委青年项目(Q201651)
更新日期/Last Update: 1900-01-01