[1]宫树一 王景续 穆胜凯.微小RNA-34a对强直性脊柱炎滑膜成纤维细胞成骨分化的影响[J].中国中医骨伤科杂志,2021,29(09):1-4.
　GONG Shuyi WANG Jingxu MU Shengkai.Efficacy of miRNA-34a on the Osteogenic Differentiation ofSynovial Fibroblasts in Ankylosing Spondylitis and Its Mechanism[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(09):1-4.
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微小RNA-34a对强直性脊柱炎滑膜成纤维细胞成骨分化的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年09期
页码:
1-4
栏目:
实验研究
出版日期:
2021-09-15

文章信息/Info

Title:
Efficacy of miRNA-34a on the Osteogenic Differentiation ofSynovial Fibroblasts in Ankylosing Spondylitis and Its Mechanism
文章编号:
1005-0205(2021)09-0001-04
作者:
宫树一1 王景续1 穆胜凯1
1沈阳市骨科医院脊柱外科(沈阳,110044)
Author(s):
GONG Shuyi1 WANG Jingxu1 MU Shengkai1
1Department of Ridge Column Surgery,Shenyang Orthopaedics Hospital,Shenyang 110044,China.
关键词:
强直性脊柱炎;  滑膜成纤维细胞;  成骨分化;  信号通路
Keywords:
ankylosing spondylitis;  synovial fibroblasts;  osteogenic differentiation;  notch signaling pathway
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨miRNA-34a对强直性脊柱炎(AS)滑膜成纤维细胞成骨分化的影响及其机制。方法:将滑膜成纤维细胞分为正常组(来自创伤性骨折患者的细胞)、AS对照组(来自AS患者的细胞)、AS+NC组(转染阴性对照并给予转化生长因子-β1(TGF-β1)和 骨形态发生蛋白2(BMP-2)处理AS细胞)和AS+miRNA-34a寡核苷酸模拟物(miRNA-34a mimics)组(转染miRNA-34a mimics并给予TGF-β1和 BMP-2处理AS细胞),采用实时-PCR检测各组细胞中miRNA-34a和成骨相关基因碱性磷酸酶(ALP)、骨钙素(OCN)、Runt相关转录因子2(Runx2)mRNA以及Notch信号通路的Notch1受体与其下游靶基因Hes1 mRNA的表达水平; 将Notch信号通路激活剂rhNF-κB加入含miRNA-34a mimics的细胞中,观察ALP、OCN和Runx2 mRNA的表达变化。结果:与正常组相比,AS对照组和AS+NC组细胞中miRNA-34a表达降低,ALP、OCN、Runx2、Notch1和Hes1 mRNA的表达水平均明显升高(P<0.05); 与AS+NC组相比,AS+miRNA-34a mimics组细胞中miRNA-34a表达升高,而ALP、OCN、Runx2、Notch1和Hes1 mRNA的表达水平均明显降低(P<0.05)。给予rhNF-κB作用后,miRNA-34a mimics对ALP、OCN和Runx2 mRNA的抑制作用减弱。结论:miRNA-34a可通过调控Notch信号通路抑制AS滑膜成纤维细胞的成骨分化能力。
Abstract:
Objective:To investigate the efficacy of miRNA-34a on osteogenic differentiation of ankylosing spondylitis(AS)synovial fibroblasts and its mechanism.Methods:Synovial fibroblasts were divided into normal group(cells from patients with traumatic fractures),AS control group(cells from patients with AS),AS+NC group(transfection negative control and treatment of AS cells with TGF-β1 and BMP-2)and AS + miRNA-34a mimics group(transfection of miRNA-34a mimics and treatment of AS cells with TGF-β1 and BMP-2).The expression levels of miRNA-34a and osteogenesis-related genes ALP,OCN,Runx2 mRNA,as well as Notch signaling pathway Notch1 receptor and its downstream target gene Hes1 mRNA in the cells of each group were detected by RT-PCR.The Notch signaling pathway activator rhNF-κB was added into the cells containing miRNA-34a mimics,and the expression changes of ALP,OCN and Runx2 mRNA were observed.Results:Compared with the normal group,the expression of miRNA-34a in AS control group and AS+NC group was decreased,and the expression levels of ALP,OCN,Runx2,Notch1 and Hes1 mRNA were significantly increased(P<0.05).Compared with AS+NC group,the expression of miRNA-34a in AS+miRNA-34a mimics group was increased,while the expression levels of ALP,OCN,Runx2,Notch1 and Hes1 mRNA were significantly decreased(P<0.05).The inhibitory efficacy of miRNA-34a mimics on ALP,OCN and Runx2 mRNA were reversed after giving rhNF-κB.Conclusion:miRNA-34a can inhibit the osteogenic differentiation of AS synovial fibroblasts by regulating the Notch signaling pathway.

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备注/Memo

备注/Memo:
基金项目:辽宁省自然科学基金指导计划项目(20180550704)
更新日期/Last Update: 1900-01-01