[1]方姝晨 邹季 汪伟 史政康 陈小微 吴曦△.三七总皂苷对股骨头坏死大鼠股骨头成骨作用影响的实验研究[J].中国中医骨伤科杂志,2020,28(06):6-9.
　FANG Shuchen ZOU Ji WANG Wei SHI Zhengkang CHEN Xiaowei WU Xi.Study of Effect of Panax Notoginseng Saponins on theOsteogenesis of Femoral Head in Rats with ONFH[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(06):6-9.
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三七总皂苷对股骨头坏死大鼠股骨头成骨作用影响的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年06期
页码:
6-9
栏目:
实验研究
出版日期:
2020-06-10

文章信息/Info

Title:
Study of Effect of Panax Notoginseng Saponins on theOsteogenesis of Femoral Head in Rats with ONFH
文章编号:
1005-0205(2020)06-0006-04
作者:
方姝晨1 邹季2 汪伟2 史政康1 陈小微1 吴曦3△
1江苏苏州市吴江区中医医院骨伤科(江苏 苏州,215200)2湖北省中医院骨伤科3十堰市人民医院(湖北医药学院附属人民医院)中医中药研究所
Author(s):
FANG Shuchen1 ZOU Ji2 WANG Wei2 SHI Zhengkang1 CHEN Xiaowei1 WU Xi3△
1Department of Orthopedics and Traumatology,Wujiang District Hospital,Suzhou 215200,Jiangsu China; 2Department of Orthopedics and Traumatology,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China; 3Institute of Traditional Chinese Medicine,Renmin Hospital,Hubei University of Medicine,Shiyan 442000,Hubei China.
关键词:
三七总皂苷;  成骨;  股骨头坏死;  大鼠
Keywords:
panax notoginseng saponins(PNS);  osteonecrosis;  osteonecrosis of the femoral head(ONFH);  rats
分类号:
R-33
文献标志码:
A
摘要:
目的:观察三七总皂苷(PNS)干预股骨头坏死(ONFH)大鼠模型后,PNS对ONFH大鼠股骨头组织成骨作用的影响。方法:36只 SPF 级雄性6个月龄SD大鼠按照随机数字表法分为对照组(Ctrl 组)、模型组(M组)和干预组(PNS组),每组大鼠12只; M组和PNS组行液氮冷冻法股骨头坏死造模,造模3个月后,M组大鼠每日腹腔注射生理盐水2 mL; PNS组大鼠腹腔注射12.0 mg/mL浓度PNS生理盐水溶液2 mL,1次/日,连续两周; Ctrl组不干预。干预后8周各组大鼠处死,采集血清、股骨头标本,行苏木精-伊红(HE)染色、OPG(Osteoprotegerin)及RANKL(Receptor Activator of NF-kB Ligand)Western Blot蛋白质电泳,ELISA法测定PINP(Procollagen Type-I N Propeptide)及TRAP-5b(Tartrate Resistant Acid Phosphatase-5b)血清浓度。结果:PNS干预液氮法ONFH大鼠可降低股骨头空骨陷窝率(P<0.01),可下调骨组织RANKL(P<0.01)及血清TRAP-5b(P<0.05)表达,上调骨组织OPG(P<0.01)表达,可提高血清PINP水平,但差异无统计学意义。结论:PNS可抑制破骨细胞活性,促进成骨,在骨坏死中发挥保护作用。
Abstract:
Objective:To explore the effect of Panax Notoginseng Saponins(PNS)on the osteogenesis of femoral head in rats with osteonecrosis of the femoral head(ONFH).Methods:36 SPF 6-month-old male SD rats were randomly divided into three groups:control group(group Ctrl),model group(group M)and group PNS.Twelve rats in group M and group PNS were treated with liquid nitrogen freezing for femoral head necrosis.Three months later,rats in group M were injected with 2 mL of saline daily for two weeks,rats in group PNS were injected with 12.0 mg/mL of PNS saline solution 2 mL intraperitoneally,the group Ctrl gave no treatment.Rats in each group were executed 8 weeks later.Serum and femoral head specimens were collected for HE staining,Western Blotting for OPG(Osteoprotegerin),RANKL(Receptor Activator of NF-kB Ligand)and ELISA was used to determine the serum concentrations of PINP(Procollagen Type-I N Propeptide),TRAP-5b(Tartrate Resistant Acid Phosphatase-5b).Results:PNS reduced the empty lacunae rate of femoral head(P<0.01),down-regulated the expression of RANKL(P<0.01),up-regulate the expression of OPG(P<0.01),reduced the level of TRAP-5b(P<0.05)in serum,and increased the level of PINP in serum in the rats with ONFH.Conclusion:PNS can inhibit the activity of osteoclasts,promote osteogenesis and play a protective role in ONFH.

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备注/Memo

备注/Memo:
(收稿日期:2019-12-20)基金项目:2015年苏州市吴江区第二批“科教兴卫”项目(WWK201523); 2016年十堰市科学技术研究与开发项目(16Y50)△通信作者 E-mail:1015411076@qq.com
更新日期/Last Update: 2020-06-10