[1]阮安民 陈谱 周俊 张晓哲 张栋 沈家豪 马玉峰 彭浩轩 杨统杰 王庆甫△.聚乙二醇沉淀法提取关节液来源外泌体实验研究[J].中国中医骨伤科杂志,2020,28(03):1-4.
　RUAN Anmin CHEN Pu ZHOU Jun ZHANG Xiaozhe ZHANG Dong SHEN JiahaoMA Yufeng PENG Haoxuan YANG Tongjie WANG Qingfu.Extraction and Identification of Exosomes Derived from Joint Effusion Based on PEG Precipitation Method[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(03):1-4.
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聚乙二醇沉淀法提取关节液来源外泌体实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年03期
页码:
1-4
栏目:
实验研究
出版日期:
2020-03-01

文章信息/Info

Title:
Extraction and Identification of Exosomes Derived from Joint Effusion Based on PEG Precipitation Method
文章编号:
1005-0205(2020)03-0001-04
作者:
阮安民1 陈谱1 周俊1 张晓哲1 张栋1 沈家豪1 马玉峰2 彭浩轩1 杨统杰1 王庆甫1; 2△
1北京中医药大学(北京,100029) 2北京中医药大学第三附属医院
Author(s):
RUAN Anmin1 CHEN Pu1 ZHOU Jun1 ZHANG Xiaozhe1 ZHANG Dong1 SHEN Jiahao1MA Yufeng2 PENG Haoxuan1 YANG Tongjie1 WANG Qingfu1; 2△
1Beijing University of Chinese Medicine,Beijing 100029,China; 2The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China.
关键词:
关节积液;  外泌体;  聚乙二醇
Keywords:
joint effusion;  exosomes;  polyethylene glycol(PEG)
分类号:
R-33
文献标志码:
A
摘要:
目的:应用聚乙二醇(PEG)沉淀法提取关节液来源外泌体,并检测外泌体的形态及分子生物学特征,为关节液来源外泌体的进一步研究奠定基础。方法:用8% PEG从关节积液中分离、提取目标颗粒,然后经透射电子显微镜观察其形态,采用纳米颗粒示踪分析仪分析其大小及纯度,蛋白质印迹法检测其标记物蛋白的表达。结果:所获目标颗粒呈圆形或椭圆形囊泡,质膜完整,大小不一,直径约为35～155 nm,且阳性表达CD9及CD63蛋白。结论:对目标颗粒的形态、大小及蛋白分析结果证实PEG沉淀法能够从关节积液中分离、提取外泌体,且操作方法简单,可为后续研究奠定基础。
Abstract:
Objective:Exosomes in joint effusion were purified by polyethylene glycol(PEG)method,their morphology and molecular biological characteristics were detected,which laid a foundation for further research on exosomes from joint effusion.Methods:The target particles were separated and purified from the joint effusion by 8% PEG.The morphology was observed by transmission electron microscopy.The size and purity of the target particles were analyzed by nanoparticle tracer analyzer.The expression of the marker protein was detected by Western blotting.Results:The target particles were round or elliptical vesicles.The plasma membrane was intact and varied in size,with a diameter of about 35～155 nm,and positively expressed CD9 and CD63 proteins.Conclusion:The morphology,size and protein analysis of the target particles confirmed that the PEG precipitation method can successfully separate and purify exosomes from joint effusion,and the operation method is simple,which lays a good foundation for subsequent research.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81874475) 北京中医药大学自主选题项目(2019-JYB-XS242)△通信作者 E-mail:qingpu-wang@sohu.com
更新日期/Last Update: 2020-03-01