[1]钱伟宏△ 沈玮 鲍青青 姚志宏 罗毅文 陆吴超 万小敏.补肾活血汤对骨髓间充质干细胞迁移及趋化因子受体4,趋化因子13 表达的影响[J].中国中医骨伤科杂志,2019,27(03):6-10,14.
　QIAN Weihong SHEN Wei BAO Qingqing YAO Zhihong LUO Yiwen LU Wuchao WAN Xiaomin.Effect of Bushen Huoxue Decoction on Bone Marrow Mesenchymal Stem Cells Migration and CXCR4,CXCL13 Expression[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(03):6-10,14.
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补肾活血汤对骨髓间充质干细胞迁移及趋化因子受体4,趋化因子13 表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年03期
页码:
6-10,14
栏目:
实验研究
出版日期:
2019-02-28

文章信息/Info

Title:
Effect of Bushen Huoxue Decoction on Bone Marrow Mesenchymal Stem Cells Migration and CXCR4,CXCL13 Expression
文章编号:
1005-0205(2019)03-0006-05
作者:
钱伟宏1△ 沈玮2 鲍青青1 姚志宏1 罗毅文2 陆吴超1 万小敏1
1浙江桐乡市中医医院(浙江 桐乡,314500)
2广州中医药大学附属骨伤科医院
△通信作者 E-mail:qgwfnpdc@126.com
Author(s):
QIAN Weihong1△ SHEN Wei2 BAO Qingqing1 YAO Zhihong1 LUO Yiwen2 LU Wuchao1 WAN Xiaomin1
1Tongxiang Chinese Medicine Hospital,Tongxiang 314500,Zhejiang China;2Affiliated Hospital of Orthopedics & Traumatology,Guangzhou University of Chinese Medicine,Guangzhou 510240,China.
关键词:
补肾活血汤;  细胞迁移;  骨髓间充质干细胞;  趋化因子13;  趋化因子受体4
Keywords:
Bushen Huoxue Decoction;  drug-containing serum;  bone marrow mesenchymal stem cells;  cell migration;  CXC chemokine 13;  CXC chemokine receptor 4
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨补肾活血汤及其拆方对骨髓间充质干细胞(BMSCs)迁移及趋化因子(CXCL)13及趋化因子受体(CXCR)4表达的影响。方法:以大鼠BMSCs为研究对象,制备含药血清,采用全骨髓贴壁法培养BMSCs。通过Transwell实验观察补肾活血汤(全方组)及其拆方(补肾组、活血组)含药血清干预BMSCs体外迁移的能力,采用PCR法检测CXCL13及CXCR4 RNA表达水平,采用Western Blot法检测CXCR4 蛋白表达水平。结果:通过培养获得高纯度的BMSCs.Transwell实验显示:全方含药血清干预的大鼠BMSCs,其迁移能力显著增强,与其它4组对比差异均有统计学意义(P<0.05)。PCR结果显示:补肾活血汤全方含药血清能显著上调大鼠BMSCs的CXCL13及CXCR4 RNA表达水平,与其它4组比较,差异均有统计学意义(P<0.05)。Western Blot结果显示:补肾活血汤全方含药血清能显著上调大鼠BMSCs的CXCR4蛋白表达水平,与其它4组比较,差异均有统计学意义(P<0.05)。结论:全方组含药血清促BMSCs体外迁移的影响较显著,结合其影响CXCL13和CXCR4的表达水平,较其单纯的活血或补肾组效果更显著,其促进BMSCs体外迁移机制可能与同时上调CXCL13及CXCR4表达,从而激活CXCL13/CXCR5信号轴及SDF-1/CXCR4信号轴有关。合理配伍促进了补肾活血汤促BMSCs体外迁移的能力。
Abstract:
Objective:To explore the effect of Bushen Huoxue Decoction(BHD)and it's separated prescription medicated on the migration of bone marrow mesenchymal stem cells(BMSCs),and expression of chemokine receptor CXCL13 and chemokine receptor CXCR4.Methods:BMSCs were used as the cell model,and were cultivated by bone marrow adherent method.Transwell assay was used to observe the effects of serum containing different dosages of BHD and it's separated prescription medicated on cell migration of BMSCs.The expression levels of chemokine receptor CXCL13 and chemokine receptor CXCR4 RNA were detected by PCR.The expression level of chemokine receptor CXCR4 protein was detected by Western Blot.Results:High purity BMSCs was obtained by culture.Transwell results showed that the whole prescription group containing serum in BHD could significantly enhance the migration ability of BMSCs in rats,and the difference was statistically significant compared with the other four groups.PCR results showed that the whole prescription group containing serum in BHD could significantly increase the level of CXCL13 and CXCR4 expression of BMSCs in rats,and the difference was statistically significant compared with the other four groups(P<0.05).Western blot results showed that the whole prescription group containing serum in BHD could significantly increase the level of CXCR4 protein expression of BMSCs in rats,and the difference was statistically significant compared with the other four group(P<0.05).Conclusion:The serum containing BHD could promote BMSCs migration,and regulate the expression level of CXCL13 and CXCR4,was statistically significant compared with the separated prescription medicated groups,and its mechanism may be related to the simultaneous increase of CXCR4 expression and CXCL13 expression,thereby activating SDF-1/CXCR4 axis and CXCL13/CXCR5 axis.Rational compatibility promotes the ability of Bushen Huoxue Decoction to promote BMSCs migration

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备注/Memo

备注/Memo:
基金项目:浙江省中医药管理局青年人才基金资助项目 (2016ZQ031)
1浙江桐乡市中医医院(浙江 桐乡,314500)
2广州中医药大学附属骨伤科医院
△通信作者 E-mail:qgwfnpdc@126.com
更新日期/Last Update: 2019-02-28