[1]邹恩苗,胡洁,高丽萍,等.电针刺激对大鼠脊髓损伤后炎症反应的影响[J].中国中医骨伤科杂志,2018,26(10):10-13.
　ZOU Enmiao,HU Jie,GAO Liping,et al.Effects of Electroacupuncture on Inflammatory Response in Spinal Cord Injury of Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(10):10-13.
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电针刺激对大鼠脊髓损伤后炎症反应的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年10期
页码:
10-13
栏目:
实验研究
出版日期:
2018-10-10

文章信息/Info

Title:
Effects of Electroacupuncture on Inflammatory Response in Spinal Cord Injury of Rats
文章编号:
1005-0205(2018)10-0010-04
作者:
邹恩苗1; 胡洁1; 高丽萍1; 屠文展1; 林海燕1△
1温州医科大学附属第二医院(育英儿童医院)(浙江 温州,325027)
Author(s):
ZOU Enmiao1; HU Jie1; GAO Liping1; TU Wenzhan1; LIN Haiyan1△
1Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang China.
关键词:
脊髓损伤;  电针;  白介素-lβ;  白介素-6;  白介素-10
Keywords:
spinal cord injury;  electroacupuncture;  interleukin-1β;  interleukin-6;  interleukin-10
分类号:
R-33
文献标志码:
A
摘要:
目的:观察不同配穴电针方式对脊髓损伤(SCI)大鼠IL-lβ,IL-6及IL-10表达的影响,探讨电针在脊髓损伤修复过程中的作用机制。方法:48只SD大鼠随机分为4 组:假手术组(12例)、对照组(12例)、夹脊电针组(12例)、督脉电针组(12例)。术后第7天对各组大鼠进行BBB运动功能评分,并采用苏木精-伊红及Nissle染色观察组织细胞形态变化,采用实时荧光定量PCR法检测白介素-lβ(IL-lβ),白介素-6(IL-6)和白介素-10(IL-10)mRNA的表达情况。结果:术后第7天对照组、夹脊电针组、督脉电针组BBB评分均低于假手术组(P<0.05),夹脊电针组、督脉电针组BBB评分均高于对照组(P<0.05); 对照组、夹脊电针组、督脉电针组IL-lβ,IL-6及IL-10 mRNA的表达含量均高于假手术组(P<0.05); 夹脊电针组、督脉电针组IL-lβ,IL-6 mRNA的表达含量均低于对照组(P<0.05); 夹脊电针组、督脉电针组IL-10 mRNA的表达含量均高于对照组(P<0.05); 夹脊电针组与督脉电针组IL-lβ,IL-6及IL-10 mRNA的表达差异无统计学意义(P>0.05)。结论:夹脊、督脉电针均可以改善大鼠SCI后下肢运动功能,降低IL-lβ和IL-6的表达,增加IL-10的表达,抑制SCI后炎症反应。
Abstract:
Objective:To observe the effect of electroacupuncture treatment(EA)at different acupoints on the expression of IL-1β,IL-6 and IL-10 in rats with spinal cord injury(SCI)and discuss the role and mechanism of electroacupuncture in the repair process of spinal cord injury.Methods:A total of 48 SD rats were randomly divided into 4 groups:sham operation group(n=12),control group(n=12),Jiaji EA group(n=12),and Du Meridian EA group(n=12).Scores of Basso Beattie Bresnahan(BBB)motor functions were assessed at 7th days after operation.Histopathological changes were observed by HE and Nissle staining.The expressions of IL-1β,IL-6 and IL-10 mRNA were detected by quantitative real time-PCR(qRT-PCR).Results:The BBB scores of the control group,Jiaji EA group,and Du meridians EA group were lower than the sham operation group on the 7th day after operation(P<0.05).The BBB scores of Jiaji EA group and Du meridians EA group were higher than the control group(P<0.05).The expression levels of IL-1β,IL-6 and IL-10 mRNA in the control group,Jiaji EA group and Du meridians EA group were higher than those in the sham operation group(P<0.05).The expression levels of IL-1β and IL-6 mRNA in the Jiaji EA group and Du meridian EA group were lower than those in the control group(P<0.05).The expression levels of IL-10 mRNA in Jiaji EA group and Du meridians EA group were high than those in the control group(P<0.05).The expressions of IL-1β,IL-6 and IL-10 mRNA in Jiaji EA group and Du meridian EA group had no statistical significance(P>0.05).Conclusion:Jiaji and Du Meridian EA could improve the motor function of the SD rats' lower limb,reduce the expression of IL-1β and IL-6 and increase expression of IL-10 and inhibit the inflammatory response after SCI.

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备注/Memo

备注/Memo:
基金项目:浙江省温州市科技局资助项目(Y20110105)
△通信作者 E-mail:linhaiyan107@163.com
更新日期/Last Update: 2018-10-10