[1]汪今朝 范筱 张俐△.活血通督汤对大鼠脊髓损伤后Ⅰ和Ⅳ型胶原蛋白表达的影响[J].中国中医骨伤科杂志,2018,26(05):1-5，9.
　WANG Jinzhao FAN Xiao ZHANG Li.Effects of Huoxue Tongdu Decoction on the Collagen Ⅰ and Collagen Ⅳ after Spinal Cord Injury[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(05):1-5，9.
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活血通督汤对大鼠脊髓损伤后Ⅰ和Ⅳ型胶原蛋白表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年05期
页码:
1-5，9
栏目:
实验研究
出版日期:
2018-05-02

文章信息/Info

Title:
Effects of Huoxue Tongdu Decoction on the Collagen Ⅰ and Collagen Ⅳ after Spinal Cord Injury
文章编号:
1005-0205(2018)05-0001-05
作者:
汪今朝1 范筱1 张俐2△
1福建中医药大学(福州,350122) 2厦门医学院
Author(s):
WANG Jinzhao1 FAN Xiao1 ZHANG Li2△
1Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 2Xiamen Medical College,Xiamen 361008,Fujian China.
关键词:
脊髓损伤;  活血通督汤;  Ⅰ型胶原蛋白;  Ⅳ型胶原蛋白
Keywords:
Keywords: spinal cord injury;  Huoxue Tongdu decoction;  collagen Ⅰ;  collagen Ⅳ
分类号:
R-33
文献标志码:
A
摘要:
目的:观察活血通督汤对大鼠脊髓损伤后Ⅰ型胶原蛋白(Collagen Ⅰ)和Ⅳ型胶原蛋白(Collagen Ⅳ)表达的影响,探讨该方对脊髓损伤后肢体运动功能恢复的作用机制。方法:将36只成年SD大鼠随机分为假手术组(n=12)、模型组(n=12)及活血通督汤组(n=12)。假手术组行椎板切除术,不损伤脊髓,模型组和活血通督汤组采用NYU脊髓损伤打击器复制脊髓损伤模型。造模成功后,假手术组和模型组给予生理盐水灌胃,活血通督汤组给予活血通督汤灌胃,给药剂量为12.6 mL/(kg·d),术后第1,3,5和7天行BBB肢体运动功能评分。采用Western blot检测Collagen Ⅰ和Collagen Ⅳ蛋白表达量,尼氏染色法观察神经细胞形态,免疫组织化学染色法检测脊髓组织中Collagen Ⅰ和Collagen Ⅳ定位表达。结果:1)术后第3天开始,活血通督汤组BBB评分高于模型组(P<0.05); 2)尼氏染色显示活血通督汤组神经元的结构形态优于模型组,神经元受损状况得到改善; 3)免疫组化和Western blot显示,与假手术组比较,模型组和活血通督汤组可见较多Collagen Ⅰ和Collagen Ⅳ表达(P<0.05),但模型组Collagen Ⅰ表达比活血通督汤组少,模型组Collagen Ⅳ表达多于活血通督汤组(P<0.05)。结论:活血通督汤促进脊髓损伤后肢体运动功能的恢复,其机制可能与其促进Collagen Ⅰ的表达且抑制Collagen Ⅳ的表达有关。
Abstract:
Abstract Objective:To observe the effect of Huoxue Tongdu decoction on the expression of collagen Ⅰ and collagen Ⅳ after spinal cord injury in rats,and to explore the mechanism of Huoxue Tongdu decoction on the recovery of limb motor function after spinal cord injury.Methods:All 36 adult SD rats were randomly divided into sham operation group,model group,Huoxue Tongdu decoction group(12 rats in each group).After established of model,the sham operation group and the model group were given normal saline every day.Huoxue Tongdu decoction group was given Huoxue Tongdu decoction,and the BBB limb motor function score was recorded on the 1st,3rd,5th and 7th day after operation.After 7 days,the tissues were gathered for Nissl's staining while collagen Ⅰ and collagen Ⅳ were detected by immunohistochemical staining and western blot.Results:1)The BBB score of Huoxue Tongdu Decoction group was higher than that of model group(P<0.05).2)Nissl's staining showed that the structure and morphology of neurons in the Huoxue Tongdu decoction group was better than that of model group with the improvement of the neuronal damage condition; 3)Compared to sham group,there were more collagen Ⅰ and collagen Ⅳ expression in the model group and Huoxue Tongdu decoction(P<0.05)and the expression of collagen I in Huoxue Tongdu decoction was more than that of model group(P<0.05),while the expression of collagen Ⅳ in model group was more than that of Huoxue Tongdu decoction(P<0.05).Conclusion:The mechanism of Huoxue Tongdu decoction to promote the recovery of limb motor function after spinal cord injury may be related to its expression of collagen Ⅰ and inhibition of collagen Ⅳ expression.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81273775) 福建省百千万领军人才项目 △通信作者 E-mail:zhanglil626@163.com
更新日期/Last Update: 2018-05-02