[1]齐英娜 谭明生 王延雷 王威 吴鑫杰 郝庆英 移平 杨峰 唐向盛.补阳还五汤对急性脊髓损伤大鼠内质网应激相关因子影响的研究[J].中国中医骨伤科杂志,2018,26(02):8-12.
 QI Yingna TAN Mingsheng WANG Yanlei WANG Wei WU Xinjie HAO Qingying YI Ping YANG Feng TANG Xiangsheng.Effect of Buyanghuanwu Decoction on the Expression of Endoplasmic Reticulum Related Factors after Acute Spinal Cord Injury in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(02):8-12.
点击复制

补阳还五汤对急性脊髓损伤大鼠内质网应激相关因子影响的研究()
分享到:

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年02期
页码:
8-12
栏目:
病证结合,从督论治
出版日期:
2018-01-15

文章信息/Info

Title:
Effect of Buyanghuanwu Decoction on the Expression of Endoplasmic Reticulum Related Factors after Acute Spinal Cord Injury in Rats
文章编号:
1005-0205(2018)02-0008-05
作者:
齐英娜1 谭明生1 王延雷1 王威1 吴鑫杰1 郝庆英1 移平1 杨峰1 唐向盛1
1中日友好医院(北京,100029)
Author(s):
QI Yingna1 TAN Mingsheng1 WANG Yanlei1 WANG Wei1 WU Xinjie1 HAO Qingying1 YI Ping1 YANG Feng1 TANG Xiangsheng1
1Department of Spine Surgery,China-Japan Friendship Hospital,Beijing 100029,China.
关键词:
脊髓损伤 督脉 补阳还五汤 内质网应激 细胞凋亡
Keywords:
Keywords: spinal cord injury buyanghuanwu decoction endoplasmic reticulum stress cell aptoposis
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨补阳还五汤灌胃治疗对大鼠急性脊髓损伤(Spinal Cord Injury,SCI)的修复作用及作用机制。方法:将SPF级3月龄Wistar雌性大鼠40只,随机分成假手术组、模型组、甲强龙组、补阳还五汤组(中药组)。造模后第1天,甲强龙组经尾静脉注射甲强龙治疗共24 h,首剂量为30 mg/kg,余以5.4 mg/(kg·h)每4 h 给药1次; 中药组以中剂量补阳还五汤颗粒剂,配制成含颗粒剂2.00 g/mL 的水溶液,每天3.5 g/kg灌胃,相当于成人用量的1倍,1次/d,共14 d.治疗后第1,3,7及14天分别以 BBB分级法评价神经功能恢复情况,Western Blot法检测SCI节段Caspase-12,Caspase-9及Caspase-3蛋白的表达。结果:各时间点 BBB评分随时间延长而上升,差异有统计学意义(P<0.01); 治疗后7 d 和14 d BBB评分比较,差异无统计学意义(P>0.05)。组间比较:中药组和甲强龙组的BBB评分高于模型组,差异有统计学意义(P<0.01); 中药组和甲强龙组比较差异无统计学意义(P>0.05)。治疗后第14天大鼠脊髓组织中Caspase-12,Caspase-9及Caspase-3 蛋白的表达:中药组和甲强龙组各蛋白表达均低于模型组,差异有统计学意义(P<0.01); 与假手术组比较,差异无统计学意义(P>0.05); 中药组和甲强龙组比较差异无统计学意义(P>0.05)。结论:围手术期以补阳还五汤治疗急性SCI,对大鼠脊髓损伤具有修复作用,其作用机制可能是通过抑制过度的内质网应激反应,下调 Caspase-12,Caspase-9及Caspase-3蛋白的表达。
Abstract:
Abstract Objective:To investigate the repair effect and mechanism of Buyanghuanwu decoction(BYHWD)on spinal cord injury(SCI)in rats.Methods:Forty female SPF Wistar rats were randomly divided into sham operation group(SO),model group,methylprednisolone group(MP),and BYHWD group,with 10 rats in each group.On the first day after model establishment,the MP group was treated with methylprednisolone injection through the tail vein for a total of 24 h.The first dose was 30 mg/kg,and then was administered with 5.4 mg/(kg·h)once,interval by every 4 h.The rats in BYHWD group were given middle dose of BYHWD at a final concentration of 2 g/mL,with BYHWD administration at 3.5 g/kg once a day(09:00)for 14 d,which was equivalent to 1 times adult dosage.Nerve functional recovery was evaluated by BBB score method at 1st,3rd,7th and 14th d after treatment,and the protein expression of Caspase-12,Caspase-9 and Caspase-3 in SCI tissue was detected by Western Blot method.Results:The results of BBB score showed that BBB score were increased as time increased(P<0.01).There was no statistically significant difference between 7 d and 14 d(P>0.05).The BBB score in BYHWD group and MP group were higher than that in model group(P<0.01).There are no significant difference between BYHWD group and MP group(P>0.05).The results of protein expression showed that the expression of Caspase-12,Caspase-9,and Caspase-3 in BYHWD group and MP group was significantly lower than that in the model group(P<0.01),which was no significant different with sham group(P>0.05).There was no significant difference between BYHWD group and MP group(P>0.05).Conclusion:BYHWD is effective for treating acute SCI during preoperative time point.Its mechanism may be through the inhibition of excessive endoplasmic reticulum stress reaction and down regulation of Caspase-12,Caspase-9 and Caspase-3 protein.

参考文献/References:

[1] Samuel AM,Grant RA,Bohl DD,et al.Delayed surgery after acute traumatic central cord syndrome is associated with reduced mortality [J].Spine,2015,40(5):349-356.
[2] 岳原亦,张扬,张一奇.Caspase家族与细胞凋亡[J].中国医疗前沿,2011,6(6):25-26.
[3] 尹洪娜,孙忠人,李全.电针夹脊穴对脊髓损伤大鼠内质网应激相关因子Caspase-12影响的实验研究[J].中医药学报,2016,44(3):33-36.
[4] 张继平,王志彬,林爱华,等.补阳还五汤对脊髓损伤大鼠脊髓组织病理学的影响[J].中国中西医结合外科杂志,2014,20(3):274-277.
[5] 张亚,饶耀剑.补阳还五汤加减治疗急性颈髓损伤临床研究[J].中医临床研究,2014,6(2):21-22.
[6] 徐志华,余勤,白月双,等.补阳还五汤联合鞘内注射间充质干细胞治疗脊髓损伤的研究[J].中华中医药学刊,2012,30(12):2726-2729.
[7] 谭明生,李显,张恩忠,等.寰枢椎脱位外科治疗与疏通督脉瘀阻相关性研究[J].中国骨伤,2012,25(11):915-919.
[8] 移平,谭明生,吕国华,等.督脉瘀阻型寰枢椎脱位手术联合中药治疗的临床研究[J].中国中医骨伤科杂志,2017,25(1):14-18.
[9] 董贤慧,谢红林,贺小平,等.补阳还五汤对脊髓损伤大鼠脊髓组织Caspase-3表达的影响[J].广东医学,2015,36(1):55-57.
[10] 徐志华,余勤,白月双,等.补阳还五汤联合鞘内注射间充质干细胞治疗脊髓损伤的研究[J].中华中医药学刊,2012,30(12):2726-2729.
[11] 谭明生,齐英娜,姜良海,等.球囊导管构建大鼠督脉瘀阻型上颈脊髓损伤模型的研究[J].中医正骨,2016,28(12):1-5.
[12] 徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,2002:202-203.
[13] Basso DM,Beattie MS,Bresnahan JC.A sensitive and reliable locamotor rating scale for open field testing in rats[J].J Neurotrauma,1995,12(1):1-21.
[14] Tan M,Dong L,Wang W,et al.Clinical application of the “pedicle exposure technique” for atlantoaxial instability patients with a narrow c1 posterior arch[J].J Spinal Disord Tech,2015,28(1):25-30.
[15] 罗禹田,彭介寿.中医治癒外伤性截瘫[J].江苏中医药,1963(2):37.
[16] 侯兆阳,陈哲,魏家森.补阳还五汤对急性脊髓损伤模型大鼠神经细胞凋亡的影响[J].浙江中西医结合杂志,2015,25(1):8-11.
[17] 董贤慧,高维娟,贺小平,等.补阳还五汤对脊髓损伤大鼠脊髓神经细胞凋亡的影响[J].承德医学院学报,2014,31(4):286-288.
[18] 赵玉鑫,王洪,杨述华,等.川芎嗪对大鼠脊髓损伤后caspase-3表达及细胞凋亡的影响[J].中国中医骨伤科杂志,2006,14(6):54-57.
[19] 周立亚,刘肖瑜,刘德玉.黄芪注射液预处理对大鼠脊髓缺血再灌注模型脊髓功能和神经细胞凋亡表达的影响[J].中国中医骨伤科杂志,2011,19(10):7-9.
[20] 冯仲锴,孙永强,刘汝银,等.芍药苷对大鼠脊髓损伤的神经保护机制[J].免疫学杂志,2016,32(5):376-381.
[21] 卜志勇,郑玲,李安军,等.红花黄素对大鼠脊髓损伤局部SOD、MDA和细胞凋亡的影响[J].湖北医药学院学报,2011,30(1):23-25.
[22] 陈向荣,游思维,金大地.BBB评分评估脊髓损伤大鼠后肢运动功能的探讨[J].中国脊柱脊髓杂志,2004(9):35-37. (收稿日期:2017-11-01)

更新日期/Last Update: 2018-02-15