[1]张亚维 颜春鲁.敦煌医方四时常服方对镉染毒大鼠骨和肾功能的影响[J].中国中医骨伤科杂志,2017,25(04):1-4.
　ZHANG Yawei YAN Chunlu.Effect of Dunhuang Sishichangfu Prescription on Bone and Renal Function in Rats Infected with Cadmium[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(04):1-4.
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敦煌医方四时常服方对镉染毒大鼠骨和肾功能的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年04期
页码:
1-4
栏目:
实验研究
出版日期:
2017-04-06

文章信息/Info

Title:
Effect of Dunhuang Sishichangfu Prescription on Bone and Renal Function in Rats Infected with Cadmium
文章编号:
1005-0205(2017)04-0001-04
作者:
张亚维1 颜春鲁2; 3
1.甘肃省中医院(兰州,730050)
2.甘肃中医药大学
3.敦煌医学与转化省部共建教育部重点实验室
Author(s):
ZHANG Yawei1 YAN Chunlu2; 3
1.Gansu Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;
2.Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;
3.Key Laboratory of Dunhuang Medical and Transformation at the Provincial and Ministerial Level of Education,Lanzhou 730000,China.
关键词:
镉染毒;  四时常服方;  骨代谢;  肾损伤
Keywords:
cadmium infected;  Sishichangfu prescription;  bone metabolism;  kidney injury
分类号:
R-33
文献标志码:
A
摘要:
目的:研究敦煌医方四时常服方对镉染毒大鼠骨和肾功能的影响。方法:将6组大鼠按照第一组(空白对照组)腹腔注射0.9%NaCl,其余五组(模型组、乙酰半胱氨酸组、四时常服方高中低剂量组)均腹腔注射0.1%氯化镉,1.5 mg/kg注射(相当于1.5 mL/kg),每周5次,共5周。染毒的同时,分别给予相应干预,乙酰半胱氨酸泡腾片溶液(5.4 mg/mL)1 mL/100 g、四时常服方各种剂量(2.6 g/mL,中1.3 g/mL,低0.65 g/mL)1 mL/100 g灌胃治疗,每天1次,连续5周。最后一次注射后,取血检测。结果:与空白对照组比较,模型组大鼠血清钙(Ca2+)、磷(P)、骨钙素显著降低,两组比较差异有统计学意义(P<0.05); 与模型组比较,乙酰半胱氨酸组、四时常服方高中低剂量组大鼠血清钙、磷、骨钙素(OC)显著增加,差异有统计学意义(P<0.05)。与空白对照组比较,模型组大鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)、尿素氮(BUN)显著升高,两组比较差异有统计学意义(P<0.05); 与模型组比较,乙酰半胱氨酸组、四时常服方高中低剂量组大鼠血清碱性磷酸酶、抗酒石酸酸性磷酸酶5b、尿素氮显著降低,差异有统计学意义(P<0.05)。结论:四时常服方对于镉染毒大鼠骨和肾功能具有一定的修复作用。
Abstract:
Objective:To study the Dunhuang Sishichangfu prescription on bone and renal function in rats infected with cadmium.Methods:The rats were divided into six groups.The first group was injected intraperitoneally with normal saline,the remaining five groups were injected intraperitoneally with 0.1% cadmium chloride,1.5 mg/kg,equivalent to 1.5 mL/kg,five times a week for 5 weeks.At the same time the rats were given corresponding intervention respectively:acetylcysteine effervescent tablets solution(5.4 mg/mL)1 mL/100 g,Sishichangfu prescription of the various doses(high 2.6 g/mL,medium 1.3 g/mL,low 0.65 g/mL)1 mL/100 g were given by gavage,once a day for five weeks.After the last injection,blood tests were taken.Results:Compared with the blank control group,the level of serum calcium(Ca2+),phosphorus(P)and osteocalcin(OC)were decreased significantly in the model group(P<0.05).Compared with the model group,the level of serum Ca2+,P and OC were increased significantly in the acetylcysteine group and Sishichangfu prescription of high and low doses groups(P<0.05).Compared with the blank control group,serum alkaline phosphatase(ALP),tartrate-resistant acid phosphatase 5b(TRACP-5b)and urea nitrogen(BUN)were significantly increased in the model group(P<0.05).Compared with the model group,the level of serum ALP,TRACP-5b and BUN were decreased significantly in the acetylcysteine group and Sishichangfu prescription of high and low doses groups(P<0.05).Conclusion:Sishichangfu prescription has a certain repair effect on bone and renal function in rats infected with cadmium.

参考文献/References:

[1] World Health Organization.Cadmium[R].Environ Health Criteria Document,1992,134:146-151.
[2] 刘爱萍,赵金垣,刘亚宁.氧自由基在急性镉中毒性肾损伤中的作用[J].中华劳动卫生职业病杂志,1998,16(1):22-25.
[3] 常秀丽,金泰度,胨亮,等.金属硫蛋白基因亚型表达在铺蓉触中作为生物标志物的研究[J].中华劳动卫生职业病杂志,2006,24(1):12-15.
[4] 李广生.地方性氟中毒发病机制[M].北京:科学出版社,2004.
[5] Staessen JA,Roels HA,Emelianov D,et a1.Environment expo-sure to cadmium,forearm bone density,and risk of fractures:prospective population study[J].Lancet,1999,353(9159):1140-1144.
[6] 史秀京,雷立健,郭建勇,等.镉致大鼠肾脏微小RNA-21表达的生物信息学分析[J].环境与职业医学,2016,33(2):113-118.
[7] 杨洋,丁天云,张明,等.SD大鼠亚慢性镉中毒试验模型的构建[J].毒理学杂志,2016,40(3):281-286.
[8] 刘艳荣,葛振丹,黄厚今.灵芝孢子粉对镉致雄性大鼠睾丸生殖细胞DNA氧化损伤的拮抗作用[J].毒理学杂志,2016,30(3):230-236.
[9] 李晔,蔺怀,张倩茹,等.矢车菊素-3-葡萄糖苷对重金属镉胁迫后大鼠氧化损伤的缓解作用[J].生态学杂志,2016,35(5):1358-1363.
[10] 史秀京,雷立健,郭建勇,等.镉致大鼠肾脏微小RNA-21表达的生物信息学分析[J].环境与职业医学,2016,33(2):113-118.
[11] Zhou X,Hao W,Shi H,et al.Calcium homeostasis disruption-A bridge connecting cadmium-induced apoptosis,autophagy and tumorigenesis [J].Oncol Res Treat,2015,38(6):311-315.
[12] Zou H,Liu X,Han T,et al.Alpha-lipoic acid protects against cadmium-induced hepatotoxicity via calcium signalling and gap junctional intercellular communication in rat hepatocytes [J].J Toxicol Sci,2015,40(4):469-477.
[13] 冯雪英,徐兆发,王飞,等.原花青素和褪黑素对亚慢性染镉大鼠肾毒性的影响[J].毒理学杂志,2009,23(2):115-117.
[14] 黎珊珊,徐兆发,李静慧,等.姜黄素对染镉6周大鼠肾损伤的影响[J].环境与职业医学杂志,2015,32(2):118-122.
[15] 李芳林,张凝宇,邓立华,等.肾损伤分子-1 mRNA在染镉肾小管细胞的表达[J].临床和实验医学杂志,2016,15(4):309-311.
[16] 詹杰,刘永林,柳承希,等.冠心康对镉染毒大鼠镉蓄积和锌钙浓度的影响[J].辽宁中医杂志,2012,39(5):929-931.
[17] 王世伟,刘文波,何桂群,等.肾主骨与骨质疏松症[J].中国中医骨伤科杂志,2011,19(8):76-78.
[18] 李宁,谢兴文,陈欣.“肾主骨、生髓”理论在骨质疏松症研究中的应用[J].中国中医骨伤科杂志,2011,19(4):70-72.
[19] 曾建春,樊粤光,刘建仁,等.“肾主骨、生髓”与骨髓间充质干细胞定向分化的研究[J].中国中医骨伤科杂志,2009,17(12):1-3.

备注/Memo

备注/Memo:
基金项目:甘肃省自然科学基金项目(1508RJZA046)
甘肃中医药大学中青年基金项目(ZQ2014-9)
敦煌医学与转化省部共建教育部重点实验室开放 基金项目(DHYX1213-004)
更新日期/Last Update: 2017-04-07