[1]徐西林 胡海 张晓峰△ 王政春 吕航 张杰 李小冬.活骨注射液治疗股骨头坏死的扫描电镜观察[J].中国中医骨伤科杂志,2017,25(02):1-5.
　XU Xilin HU Hai ZHANG Xiaofeng WANG Zhengchun L Hang ZHANG Jie LI Xiaodong.Scanning Electron Microscopic Observation on the Treatment of Femur Head Necrosis with Huogu Injection[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(02):1-5.
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活骨注射液治疗股骨头坏死的扫描电镜观察()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年02期
页码:
1-5
栏目:
实验研究
出版日期:
2017-02-15

文章信息/Info

Title:
Scanning Electron Microscopic Observation on the Treatment of Femur Head Necrosis with Huogu Injection
文章编号:
1005-0205(2017)02-0001-05
作者:
徐西林1 胡海1 张晓峰1△ 王政春2 吕航1 张杰1 李小冬1
1黑龙江中医药大学附属第二医院(哈尔滨,150001) 2黑龙江中医药大学 △通信作者 E-mail:zxfeng9919@163.com
Author(s):
XU Xilin1 HU Hai1 ZHANG Xiaofeng1△ WANG Zhengchun2 LÜ Hang1 ZHANG Jie1 LI Xiaodong1
1The Second Hospital,Affiliated to Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China; 2Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China.
关键词:
股骨头坏死;  活骨注射液;  模型; 动物;  扫描电镜;  动物实验
Keywords:
femur head necrosis;  Huogu injection;  models;  animal;  scanning electron microscope;  animal experimentation
分类号:
R-33
文献标志码:
A
摘要:
目的:观察活骨注射液股骨头灌注对兔液氮冷冻法股骨头坏死模型的扫描电镜下改变。方法:将34只新西兰大白兔随机分为空白组2只,造模组32只,造模组采用液氮冷冻法进行股骨头坏死造模,造模成功1周后随机抽取2只造模组动物与2只空白组动物进行电镜观察比较坏死情况; 造模组随机分为模型组、对照组、实验组各10只,分别以生理盐水、冠心宁注射液和活骨注射液进行髋关节腔灌注,药物干预开始1,3,6,9,12周后,分别从各组随机选取2只动物以空气栓塞法处死后取出坏死股骨头,采用扫描电镜观察标本微观结构变化情况。结果:实验组股骨头坏死恢复明显较对照组和模型组快且完整,实验组股骨头塌陷程度相对较轻,骨坏死修复过程中增生的纤维出现更早,纤维束的塑形时间更短,软骨下层骨组织排列更紧密,结构恢复更趋近正常,骨陷窝结构出现更多,股骨头坏死区域恢复面积更大,骨坏死区恢复更接近正常骨组织。结论:中药髋关节腔灌注对股骨头坏死的恢复有积极意义,且相对于活血化瘀法及补肾活血法对股骨头坏死的修复作用更强。
Abstract:
Objective:To observe the changes of scanning electron microscope of femur head perfusion with Huogu injection in the treatment of femur head necrosis model induced by liquid nitrogen freezing in rabbits.Methods:All 34 New Zealand white rabbits were randomly divided into blank group(n=2)and modeling group(n= 32).The modeling group was molded with femur head necrosis induced by liquid nitrogen freezing.After 1 week of successful modeling,2 rabbits in the modeling group and 2 in the blank group were randomly selected to observe the necrosis by electron microscopy.The modeling group was randomly divided into model group,control group,and experimental group(10 rabbits in each group),and were perfused hip joint cavity with saline solution,Guanxinning injection and Huogu injection respectively.At the 1st,3rd,6th,9th and 12th week after drug intervention,2 rabbits were randomly selected from each group to remove the necrotic femur head by air embolization.The microstructural changes of the specimens were observed by scanning electron microscope.Results:The recovery of femur head necrosis was significantly faster and more complete in the experimental group than that in the control group and the model group.And in the experimental group,the collapse degree of the femur head was relatively slight,the proliferating fibers appeared earlier in the osteonecrosis repair process,the time of the fibrous capsule was shorter,the subchondral bone was arranged more closely,the structure recovered more normal,the area of the femur head necrosis was larger,and the area of osteonecrosis was much closer to the normal bone tissue.Conclusion:Hip joint perfusion of traditional Chinese medicine on the restoration of femur head necrosis has a positive significance,but compared to Huoxuehuayu method,the femur head necrosis recovery of the Bushenhuoxue method was stronger.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81173276)
更新日期/Last Update: 2017-09-08