[1]王苗苗 王玲 王富强 许志洋 孙志岭△.强直性脊柱炎寒湿痹阻证血清蛋白质组学研究[J].中国中医骨伤科杂志,2015,23(12):9-11.
　WANG Miaomiao WANG Ling WANG Fuqiang XU Zhiyang SUN Zhiling.Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Cold-Dampness Arthralgia Spasm Syndrome[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(12):9-11.
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强直性脊柱炎寒湿痹阻证血清蛋白质组学研究
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年12期
页码:
9-11
栏目:
临床研究
出版日期:
2015-12-08

文章信息/Info

Title:
Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Cold-Dampness Arthralgia Spasm Syndrome
文章编号:
1005-0205(2015)12-0009-03
作者:
王苗苗1 王玲2 王富强2 许志洋2 孙志岭1△
1.南京中医药大学护理学院(南京,210023)
2.南京医科大学分析测试中心
△.通信作者 E-mail:szl1970@126.com
Author(s):
WANG Miaomiao1 WANG Ling2 WANG Fuqiang2 XU Zhiyang2 SUN Zhiling1△
1.College of Nursing, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China;
2.Analytical and Testing Center, Nanjing Medical University,Nanjing 210029,China.
关键词:
强直性脊柱炎;  寒湿痹阻证;  双向凝胶电泳;  质谱;  血清
Keywords:
ankylosing spondylitis;  cold-dampness arthralgia spasm syndrome;  two-dimensional gel electrophoresis;  mass spectrometry;  serum
分类号:
R593.23
文献标志码:
A
摘要:
目的:基于蛋白质组学技术,开展强直性脊柱炎中医寒湿痹阻证的研究。方法:选取强直性脊柱炎中医寒湿痹阻证、湿热痹阻证、正常对照共3组血清各30例,对各组进行双向电泳,比较寒湿痹阻证组和其它两组电泳图谱,寻找共同差异蛋白点,质谱鉴定与数据库查询得到差异蛋白。结果:获得聚腺苷二磷酸核糖聚合酶1、弗林蛋白酶、血管内皮钙黏蛋白、激肽原1、血管紧张素原等5个强直性脊柱炎寒湿痹阻证差异蛋白,涉及酶类、粘附分子、凝血因子、肽类激素等。结论:这些蛋白可能涉及强直性脊柱炎寒湿痹阻证的发病和病理过程,为进一步研究其预后分子标志物、诊断以及治疗提供了依据。
Abstract:
Objective: Proteomic technique was applied to analyze ankylosing spondylitis(AS)patients with cold-dampness arthralgia spasm syndrome. Methods: Serum proteins of AS patients with cold-dampness arthralgia spasm syndrome group(n=30)was compared with that of normal control group(n=30)and AS patients with damp-heat arthralgia spasm syndrome group(n=30)respectively by two-dimensional gel electrophoresis; the common differential protein spots of cold-dampness arthralgia spasm syndrome were analyzed by mass spectrometry. Results: Five common differential protein spots were selected and identified by mass spectrometry. They are poly(ADP-ribose)polymerase 1, furin, VE-cadherin, kininogen 1, angiotensinogen and related to enzyme, adhesion molecule, coagulation factor, peptide hormone, and so on. Conclusion: These proteins are implicated in the pathogenesis and pathological processes of AS with cold-dampness arthralgia spasm syndrome, and provide a basis for further studies of the diagnosis, prognostic markers, or treatment of AS patients with cold-dampness arthralgia spasm syndrome.

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备注/Memo

备注/Memo:
收稿日期:2015-09-03
基金项目:国家自然科学基金资助项目(81173158)
江苏省高校自然科学基金项目(13KJD360002)
江苏省高校“青蓝工程”项目(2014)
江苏省高校优秀中青年教师赴境外研修项目(2013)
更新日期/Last Update: 2015-12-15